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Determination of the formal redox potentials of the cyanhaemoglobin/cyanmethaemoglobin and the myoglobin/metmyoglobin couples at neutral pH.
Bioelectrochemistry 2018; 120:83-86B

Abstract

Determination of a representative formal redox potential of the Fe(II)/Fe(III) redox couple in cyanhaemoglobin, at pH=7 and related to the state in solution, was the objective of this work. It was achieved at low concentrations of the protein (5μM) to circumvent undesired adsorption. Square-wave voltammetry instead of classical cyclic voltammetry was applied because this method is more sensitive and provides information on the formal redox potential and reversibility, even for rapid processes. We obtained E°'=-0.12±0.01V for cyanhaemoglobin and E°'=-0.10±0.01V, vs. SHE, for myoglobin in comparison. These values differ by only 20mV because the two Fe(II)/Fe(III) redox centres are embedded in closely resembling chemical environments. The small difference is probably owed to the additional axially coordinating cyanide ligand in cyanmethaemoglobin which slightly favours the Fe(III) state in the haem macrocycle.

Authors+Show Affiliations

Institute of Chemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.Institute of Chemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.Institute of Inorganic Chemistry, ETH Zurich, Vladimir-Prelog-Weg 1-2, 8093 Zurich, Switzerland. Electronic address: kissner@inorg.chem.ethz.ch.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29197256

Citation

Mahmoudi, Leila, et al. "Determination of the Formal Redox Potentials of the Cyanhaemoglobin/cyanmethaemoglobin and the Myoglobin/metmyoglobin Couples at Neutral PH." Bioelectrochemistry (Amsterdam, Netherlands), vol. 120, 2018, pp. 83-86.
Mahmoudi L, Zelder F, Kissner R. Determination of the formal redox potentials of the cyanhaemoglobin/cyanmethaemoglobin and the myoglobin/metmyoglobin couples at neutral pH. Bioelectrochemistry. 2018;120:83-86.
Mahmoudi, L., Zelder, F., & Kissner, R. (2018). Determination of the formal redox potentials of the cyanhaemoglobin/cyanmethaemoglobin and the myoglobin/metmyoglobin couples at neutral pH. Bioelectrochemistry (Amsterdam, Netherlands), 120, pp. 83-86. doi:10.1016/j.bioelechem.2017.11.012.
Mahmoudi L, Zelder F, Kissner R. Determination of the Formal Redox Potentials of the Cyanhaemoglobin/cyanmethaemoglobin and the Myoglobin/metmyoglobin Couples at Neutral PH. Bioelectrochemistry. 2018;120:83-86. PubMed PMID: 29197256.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of the formal redox potentials of the cyanhaemoglobin/cyanmethaemoglobin and the myoglobin/metmyoglobin couples at neutral pH. AU - Mahmoudi,Leila, AU - Zelder,Felix, AU - Kissner,Reinhard, Y1 - 2017/11/27/ PY - 2017/09/18/received PY - 2017/11/23/revised PY - 2017/11/23/accepted PY - 2017/12/3/pubmed PY - 2018/5/1/medline PY - 2017/12/3/entrez KW - Cyanhemoglobin KW - Formal redox potential KW - Graphite electrode KW - Myoglobin KW - Square-wave voltammetry SP - 83 EP - 86 JF - Bioelectrochemistry (Amsterdam, Netherlands) JO - Bioelectrochemistry VL - 120 N2 - Determination of a representative formal redox potential of the Fe(II)/Fe(III) redox couple in cyanhaemoglobin, at pH=7 and related to the state in solution, was the objective of this work. It was achieved at low concentrations of the protein (5μM) to circumvent undesired adsorption. Square-wave voltammetry instead of classical cyclic voltammetry was applied because this method is more sensitive and provides information on the formal redox potential and reversibility, even for rapid processes. We obtained E°'=-0.12±0.01V for cyanhaemoglobin and E°'=-0.10±0.01V, vs. SHE, for myoglobin in comparison. These values differ by only 20mV because the two Fe(II)/Fe(III) redox centres are embedded in closely resembling chemical environments. The small difference is probably owed to the additional axially coordinating cyanide ligand in cyanmethaemoglobin which slightly favours the Fe(III) state in the haem macrocycle. SN - 1878-562X UR - https://www.unboundmedicine.com/medline/citation/29197256/Determination_of_the_formal_redox_potentials_of_the_cyanhaemoglobin/cyanmethaemoglobin_and_the_myoglobin/metmyoglobin_couples_at_neutral_pH L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5394(17)30475-9 DB - PRIME DP - Unbound Medicine ER -