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In Vitro Anti-inflammatory Effects of Equisetum arvense Are Not Solely Mediated by Silica.
Planta Med 2018; 84(8):519-526PM

Abstract

Equisetum arvense, known as common horsetail, is used for the treatment of inflammatory diseases and is the plant with the highest concentration of silica. Yet it is unknown if the medicinal properties are mediated by its silica content. In the current study, optimal conditions for silica-rich horsetail preparations were identified. Bioactivity of the preparations was analyzed in vitro using flow cytometry-based activity and functionality profiling of primary human lymphocytes as well as cytokine measurement using a classical ELISA technique. Experiments revealed that horsetail preparations suppress activation and proliferation of lymphocytes by an interleukin-2-dependent mechanism. The effect increased with the silica concentration in the decoctions. Lymphocytes' polyfunctionality was also influenced, shown by a downregulation of IFN-γ. Analytical profiling by HPLC-UV-MS and bioactivity testing revealed relevant immunosuppressive concentrations of a component that has been identified as isoquercitrin. Our results show that both silica and isoquercitrin are active compounds of horsetail preparations.

Authors+Show Affiliations

Center for Complementary Medicine, Institute for Infection Prevention and Hospital Epidemiology, Faculty of Medicine, University of Freiburg.Division of Pharmaceutical Biology, University of Basel, Switzerland.Salumed-Verlag GmbH, Gemeinschaftskrankenhaus Havelhöhe, Berlin.Pharmacy of Freiburg University Medical Center, Freiburg.Center for Complementary Medicine, Institute for Infection Prevention and Hospital Epidemiology, Faculty of Medicine, University of Freiburg.Center for Complementary Medicine, Institute for Infection Prevention and Hospital Epidemiology, Faculty of Medicine, University of Freiburg.Center for Complementary Medicine, Institute for Infection Prevention and Hospital Epidemiology, Faculty of Medicine, University of Freiburg.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29202511

Citation

Steinborn, Carmen, et al. "In Vitro Anti-inflammatory Effects of Equisetum Arvense Are Not Solely Mediated By Silica." Planta Medica, vol. 84, no. 8, 2018, pp. 519-526.
Steinborn C, Potterat O, Meyer U, et al. In Vitro Anti-inflammatory Effects of Equisetum arvense Are Not Solely Mediated by Silica. Planta Med. 2018;84(8):519-526.
Steinborn, C., Potterat, O., Meyer, U., Trittler, R., Stadlbauer, S., Huber, R., & Gründemann, C. (2018). In Vitro Anti-inflammatory Effects of Equisetum arvense Are Not Solely Mediated by Silica. Planta Medica, 84(8), pp. 519-526. doi:10.1055/s-0043-123075.
Steinborn C, et al. In Vitro Anti-inflammatory Effects of Equisetum Arvense Are Not Solely Mediated By Silica. Planta Med. 2018;84(8):519-526. PubMed PMID: 29202511.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In Vitro Anti-inflammatory Effects of Equisetum arvense Are Not Solely Mediated by Silica. AU - Steinborn,Carmen, AU - Potterat,Olivier, AU - Meyer,Ulrich, AU - Trittler,Rainer, AU - Stadlbauer,Sven, AU - Huber,Roman, AU - Gründemann,Carsten, Y1 - 2017/12/04/ PY - 2017/12/5/pubmed PY - 2018/9/11/medline PY - 2017/12/5/entrez SP - 519 EP - 526 JF - Planta medica JO - Planta Med. VL - 84 IS - 8 N2 - Equisetum arvense, known as common horsetail, is used for the treatment of inflammatory diseases and is the plant with the highest concentration of silica. Yet it is unknown if the medicinal properties are mediated by its silica content. In the current study, optimal conditions for silica-rich horsetail preparations were identified. Bioactivity of the preparations was analyzed in vitro using flow cytometry-based activity and functionality profiling of primary human lymphocytes as well as cytokine measurement using a classical ELISA technique. Experiments revealed that horsetail preparations suppress activation and proliferation of lymphocytes by an interleukin-2-dependent mechanism. The effect increased with the silica concentration in the decoctions. Lymphocytes' polyfunctionality was also influenced, shown by a downregulation of IFN-γ. Analytical profiling by HPLC-UV-MS and bioactivity testing revealed relevant immunosuppressive concentrations of a component that has been identified as isoquercitrin. Our results show that both silica and isoquercitrin are active compounds of horsetail preparations. SN - 1439-0221 UR - https://www.unboundmedicine.com/medline/citation/29202511/In_Vitro_Anti_inflammatory_Effects_of_Equisetum_arvense_Are_Not_Solely_Mediated_by_Silica_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-0043-123075 DB - PRIME DP - Unbound Medicine ER -