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Genetic Polymorphisms of 21 STR Loci in Hunan Province-based Han Population.
Fa Yi Xue Za Zhi. 2016 Oct; 32(5):356-362.FY

Abstract

OBJECTIVES

To investigate the genetic polymorphisms of 21 short tandem repeat （STR） loci （D3S1358, D13S317, D7S820, D16S539, Penta E, D2S441, TPOX, TH01, D2S1338, CSF1PO, Penta D, D10S1248, D19S433, vWA, D21S11, D18S51, D6S1043, D8S1179, D5S818, D12S391 and FGA）.

METHODS

A total of 560 blood samples were collected from unrelated healthy individuals of Han population in Hunan Province. Chelex-100 extraction method was applied to the extraction of genomic DNA, and an AGCU EX22 Kit and 9700 STR amplification was used in amplification reactions. The products were separated and analyzed on 310 Genetic Analyzer.

RESULTS

A total of 248 alleles were observed, the allelic frequencies ranging from 0.001 to 0.518. Observation of genotype distributions for each locus showed no deviations from Hardy-Weinberg equilibrium except Penta E （P=0.023）. The combined power of discrimination, combined power of exclusion, and combined matching probability of the 21 STR loci were approximately 0.999 999 999 999 999 999 999 999 8, 0.999 999 998, and 1.36×10⁻²⁵, respectively.

CONCLUSIONS

The 21 STR loci show high polymorphisms in the Han population, which can provide valuable data and a theoretical basis for forensic individual identification and paternity testing.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Zou Y
Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, China.
Guo JJ
Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, China.
Li QP
Xiangya School of Medicine, Central South University, Changsha 410013, China.
Zuo DH
Xiangya School of Medicine, Central South University, Changsha 410013, China.
Liu JS
Department of Internal Medicine, Affiliated Hospital of Inner Mongolia University for Nationalities, Tongliao 028000, China.
Guo YD
Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, China.
Yan J
Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, China.
Zha L
Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, China.
Cai JF
Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, China.
Lan LM
Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, China.

MeSH

AllelesAsian PeopleChinaDNA FingerprintingGene FrequencyGenetic TestingGenetics, PopulationGenotypeHumansMicrosatellite RepeatsPolymerase Chain ReactionPolymorphism, GeneticProbability

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29205006

Citation

Zou, Y, et al. "Genetic Polymorphisms of 21 STR Loci in Hunan Province-based Han Population." Fa Yi Xue Za Zhi, vol. 32, no. 5, 2016, pp. 356-362.
Zou Y, Guo JJ, Li QP, et al. Genetic Polymorphisms of 21 STR Loci in Hunan Province-based Han Population. Fa Yi Xue Za Zhi. 2016;32(5):356-362.
Zou, Y., Guo, J. J., Li, Q. P., Zuo, D. H., Liu, J. S., Guo, Y. D., Yan, J., Zha, L., Cai, J. F., & Lan, L. M. (2016). Genetic Polymorphisms of 21 STR Loci in Hunan Province-based Han Population. Fa Yi Xue Za Zhi, 32(5), 356-362. https://doi.org/10.3969/j.issn.1004-5619.2016.05.010
Zou Y, et al. Genetic Polymorphisms of 21 STR Loci in Hunan Province-based Han Population. Fa Yi Xue Za Zhi. 2016;32(5):356-362. PubMed PMID: 29205006.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic Polymorphisms of 21 STR Loci in Hunan Province-based Han Population. AU - Zou,Y, AU - Guo,J J, AU - Li,Q P, AU - Zuo,D H, AU - Liu,J S, AU - Guo,Y D, AU - Yan,J, AU - Zha,L, AU - Cai,J F, AU - Lan,L M, Y1 - 2016/10/25/ PY - 2015/07/20/received PY - 2017/12/6/entrez PY - 2016/10/1/pubmed PY - 2018/2/16/medline KW - Han nationality KW - Hunan KW - forensic genetics KW - polymorphism, genetic KW - short tandem repeat sequences SP - 356 EP - 362 JF - Fa yi xue za zhi JO - Fa Yi Xue Za Zhi VL - 32 IS - 5 N2 - OBJECTIVES: To investigate the genetic polymorphisms of 21 short tandem repeat （STR） loci （D3S1358, D13S317, D7S820, D16S539, Penta E, D2S441, TPOX, TH01, D2S1338, CSF1PO, Penta D, D10S1248, D19S433, vWA, D21S11, D18S51, D6S1043, D8S1179, D5S818, D12S391 and FGA）. METHODS: A total of 560 blood samples were collected from unrelated healthy individuals of Han population in Hunan Province. Chelex-100 extraction method was applied to the extraction of genomic DNA, and an AGCU EX22 Kit and 9700 STR amplification was used in amplification reactions. The products were separated and analyzed on 310 Genetic Analyzer. RESULTS: A total of 248 alleles were observed, the allelic frequencies ranging from 0.001 to 0.518. Observation of genotype distributions for each locus showed no deviations from Hardy-Weinberg equilibrium except Penta E （P=0.023）. The combined power of discrimination, combined power of exclusion, and combined matching probability of the 21 STR loci were approximately 0.999 999 999 999 999 999 999 999 8, 0.999 999 998, and 1.36×10⁻²⁵, respectively. CONCLUSIONS: The 21 STR loci show high polymorphisms in the Han population, which can provide valuable data and a theoretical basis for forensic individual identification and paternity testing. SN - 1004-5619 UR - https://www.unboundmedicine.com/medline/citation/29205006/Genetic_Polymorphisms_of_21_STR_Loci_in_Hunan_Province_based_Han_Population_ DB - PRIME DP - Unbound Medicine ER -