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Production of antibodies to gliadin in intestinal mucosa of patients with coeliac disease: a study at the single cell level.
Gut. 1989 Jan; 30(1):72-7.Gut

Abstract

Lymphocytes obtained after enzymatic digestion of intestinal biopsies from patients with coeliac disease were examined for the presence of gliadin specific antibody secreting cells by means of the ELISPOT technique. This technique permits enumeration of gliadin antibody secreting immunocytes, differentiated with regard to immunoglobulin class. Patients with coeliac disease were found to have high (834/10(6) cells) numbers of antigliadin spot forming cells (SFC) in gut mucosa. IgG and IgM antigliadin antibody secreting cells were infrequently shown whereas IgA antigliadin SFC predominated in all patients tested (average 68% of total SFC). Ten control patients were investigated in parallel with the coeliac patients and showed only low numbers of gliadin antibody secreting cells in gut mucosa (49/10(6) isolated cells). Antigliadin antibody secretion by peripheral blood mononuclear cells was shown in only two of six coeliac patients tested and in none of the control patients. The findings suggest that the intestinal mucosa is a major site for antigliadin antibody production and that IgA is the dominating Ig-class of these antibodies. The high sensitivity and accuracy of the ELISPOT technique may provide a useful instrument for future studies of antibody production and regulation of the gut immune response to gluten and other alimentary antigens in coeliac and other intestinal diseases.

Authors+Show Affiliations

Department of Medical Microbiology, University of Göteborg, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2920930

Citation

Lycke, N, et al. "Production of Antibodies to Gliadin in Intestinal Mucosa of Patients With Coeliac Disease: a Study at the Single Cell Level." Gut, vol. 30, no. 1, 1989, pp. 72-7.
Lycke N, Kilander A, Nilsson LA, et al. Production of antibodies to gliadin in intestinal mucosa of patients with coeliac disease: a study at the single cell level. Gut. 1989;30(1):72-7.
Lycke, N., Kilander, A., Nilsson, L. A., Tarkowski, A., & Werner, N. (1989). Production of antibodies to gliadin in intestinal mucosa of patients with coeliac disease: a study at the single cell level. Gut, 30(1), 72-7.
Lycke N, et al. Production of Antibodies to Gliadin in Intestinal Mucosa of Patients With Coeliac Disease: a Study at the Single Cell Level. Gut. 1989;30(1):72-7. PubMed PMID: 2920930.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Production of antibodies to gliadin in intestinal mucosa of patients with coeliac disease: a study at the single cell level. AU - Lycke,N, AU - Kilander,A, AU - Nilsson,L A, AU - Tarkowski,A, AU - Werner,N, PY - 1989/1/1/pubmed PY - 1989/1/1/medline PY - 1989/1/1/entrez SP - 72 EP - 7 JF - Gut JO - Gut VL - 30 IS - 1 N2 - Lymphocytes obtained after enzymatic digestion of intestinal biopsies from patients with coeliac disease were examined for the presence of gliadin specific antibody secreting cells by means of the ELISPOT technique. This technique permits enumeration of gliadin antibody secreting immunocytes, differentiated with regard to immunoglobulin class. Patients with coeliac disease were found to have high (834/10(6) cells) numbers of antigliadin spot forming cells (SFC) in gut mucosa. IgG and IgM antigliadin antibody secreting cells were infrequently shown whereas IgA antigliadin SFC predominated in all patients tested (average 68% of total SFC). Ten control patients were investigated in parallel with the coeliac patients and showed only low numbers of gliadin antibody secreting cells in gut mucosa (49/10(6) isolated cells). Antigliadin antibody secretion by peripheral blood mononuclear cells was shown in only two of six coeliac patients tested and in none of the control patients. The findings suggest that the intestinal mucosa is a major site for antigliadin antibody production and that IgA is the dominating Ig-class of these antibodies. The high sensitivity and accuracy of the ELISPOT technique may provide a useful instrument for future studies of antibody production and regulation of the gut immune response to gluten and other alimentary antigens in coeliac and other intestinal diseases. SN - 0017-5749 UR - https://www.unboundmedicine.com/medline/citation/2920930/Production_of_antibodies_to_gliadin_in_intestinal_mucosa_of_patients_with_coeliac_disease:_a_study_at_the_single_cell_level_ L2 - https://gut.bmj.com/lookup/pmidlookup?view=long&pmid=2920930 DB - PRIME DP - Unbound Medicine ER -