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Dengue serotype-specific immune response in Aedes aegypti and Aedes albopictus.

Abstract

BACKGROUND

Dengue viruses (DENV) are considered one of the most important emerging pathogens and dengue disease is a global health threat. The geographic expansion of dengue viruses has led to co-circulation of all four dengue serotypes making it imperative that new DENV control strategies be devised.

OBJECTIVES

Here we characterize dengue serotype-specific innate immune responses in Aedes aegypti and Aedes albopictus using DENV from Puerto Rico (PR).

METHODS

Ae. aegypti and Ae. albopictus were infected with dengue serotype 1 and 2 isolated from Puerto Rico. DENV infected mosquito samples were collected and temporal change in expression of selected innate immune response pathway genes analyzed by quantitative real time PCR.

FINDINGS

The Toll pathway is involved in anti-dengue response in Ae. aegypti, and Ae. albopictus. Infections with PR DENV- 1 elicited a stronger response from genes of the Toll immune pathway than PR DENV-2 in Ae. aegypti but in infected Ae. albopictus expression of Toll pathway genes tended to be similar between the serotypes. Two genes (a ribosomal S5 protein gene and a nimrod-like gene) from Ae. albopictus were expressed in response to DENV.

MAIN CONCLUSIONS

These studies revealed a role for antiviral genes in DENV serotype-specific interactions with DENV vectors, demonstrated that infections with DENV-2 can modulate the Toll immune response pathway in Ae. aegypti and elucidated candidate molecules that might be used to interfere with serotype specific vector-virus interactions.

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  • Authors+Show Affiliations

    ,

    University of Florida, Department of Entomology and Nematology, Florida Medical Entomology Laboratory, Vero Beach, FL, USA.

    ,

    University of Florida, Department of Entomology and Nematology, Florida Medical Entomology Laboratory, Vero Beach, FL, USA.

    University of Florida, Department of Entomology and Nematology, Florida Medical Entomology Laboratory, Vero Beach, FL, USA.

    Source

    Memorias do Instituto Oswaldo Cruz 112:12 2017 Dec pg 829-837

    MeSH

    Aedes
    Animals
    Dengue Virus
    Insect Vectors
    Real-Time Polymerase Chain Reaction

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    29211244