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Genetic classification of Aegilops columnaris Zhuk. (2n=4x=28, UcUcXcXc) chromosomes based on FISH analysis and substitution patterns in common wheat × Ae. columnaris introgressive lines.
Genome. 2018 Feb; 61(2):131-143.G

Abstract

Aegilops columnaris is a tetraploid species originated from Ae. umbellulata (2n=2x=14, UU) and a yet unknown diploid grass species. Although Ae. columnaris possesses some agronomically valuable traits, such as heat and drought tolerance and resistance to pests, it has never been used in wheat breeding because of difficulties in producing hybrids and a lack of information on the relationships between Ae. columnaris and common wheat chromosomes. In this paper, we report the development of 57 wheat - Ae. columnaris introgressive lines covering 8 of the14 chromosomes of Aegilops. Based on substitution spectra of hybrids and the results of FISH analysis of the parental Ae. columnaris line with seven DNA probes, we have developed the genetic nomenclature of the Uc and Xc chromosomes. Genetic groups and genome affinities were established for 11 of 14 chromosomes; the classification of the remaining three chromosomes remains unsolved. Each Ae. columnaris chromosome was characterized on the basis of C-banding pattern and the distribution of seven DNA sequences. Introgression processes were shown to depend on the parental wheat genotype and the level of divergence of homoeologous chromosomes. We found that lines carrying chromosome 5Xc are resistant to leaf rust; therefore, this chromosome could possess novel resistance genes that have never been utilized in wheat breeding.

Authors+Show Affiliations

a Vavilov Institute of General Generics, Russian Academy of Sciences, Gubkina Str. 3, GSP-1, Moscow 119991, Russia. b Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilova str. 32, GSP-1, Moscow 119991, Russia.c Leibniz-Institute of Plant Genetics and Crop Plant Research (IPK), Chromosome Structure and Function Laboratory, Corrensstraβe 3, 06466 Gatersleben, Germany.a Vavilov Institute of General Generics, Russian Academy of Sciences, Gubkina Str. 3, GSP-1, Moscow 119991, Russia.d Agricultural Research Institute of South-East Regions, Russian Academy of Agricultural Sciences, Tulaikova Str. 7, Saratov 140010, Russia.d Agricultural Research Institute of South-East Regions, Russian Academy of Agricultural Sciences, Tulaikova Str. 7, Saratov 140010, Russia.b Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilova str. 32, GSP-1, Moscow 119991, Russia.a Vavilov Institute of General Generics, Russian Academy of Sciences, Gubkina Str. 3, GSP-1, Moscow 119991, Russia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29216443

Citation

Badaeva, E D., et al. "Genetic Classification of Aegilops Columnaris Zhuk. (2n=4x=28, UcUcXcXc) Chromosomes Based On FISH Analysis and Substitution Patterns in Common Wheat × Ae. Columnaris Introgressive Lines." Genome, vol. 61, no. 2, 2018, pp. 131-143.
Badaeva ED, Ruban AS, Shishkina AA, et al. Genetic classification of Aegilops columnaris Zhuk. (2n=4x=28, UcUcXcXc) chromosomes based on FISH analysis and substitution patterns in common wheat × Ae. columnaris introgressive lines. Genome. 2018;61(2):131-143.
Badaeva, E. D., Ruban, A. S., Shishkina, A. A., Sibikeev, S. N., Druzhin, A. E., Surzhikov, S. A., & Dragovich, A. Y. (2018). Genetic classification of Aegilops columnaris Zhuk. (2n=4x=28, UcUcXcXc) chromosomes based on FISH analysis and substitution patterns in common wheat × Ae. columnaris introgressive lines. Genome, 61(2), 131-143. https://doi.org/10.1139/gen-2017-0186
Badaeva ED, et al. Genetic Classification of Aegilops Columnaris Zhuk. (2n=4x=28, UcUcXcXc) Chromosomes Based On FISH Analysis and Substitution Patterns in Common Wheat × Ae. Columnaris Introgressive Lines. Genome. 2018;61(2):131-143. PubMed PMID: 29216443.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic classification of Aegilops columnaris Zhuk. (2n=4x=28, UcUcXcXc) chromosomes based on FISH analysis and substitution patterns in common wheat × Ae. columnaris introgressive lines. AU - Badaeva,E D, AU - Ruban,A S, AU - Shishkina,A A, AU - Sibikeev,S N, AU - Druzhin,A E, AU - Surzhikov,S A, AU - Dragovich,A Yu, Y1 - 2017/12/07/ PY - 2017/12/8/pubmed PY - 2018/8/2/medline PY - 2017/12/8/entrez KW - Aegilops columnaris KW - C-banding KW - FISH KW - Uc genome KW - Xc genome KW - addition KW - blé tendre KW - chromosome KW - classification génétique des chromosomes KW - coloration des bandes C KW - common wheat KW - genetic classification of chromosomes KW - génome Uc KW - génome Xc KW - homoeology KW - homéologie KW - introgression KW - substitution SP - 131 EP - 143 JF - Genome JO - Genome VL - 61 IS - 2 N2 - Aegilops columnaris is a tetraploid species originated from Ae. umbellulata (2n=2x=14, UU) and a yet unknown diploid grass species. Although Ae. columnaris possesses some agronomically valuable traits, such as heat and drought tolerance and resistance to pests, it has never been used in wheat breeding because of difficulties in producing hybrids and a lack of information on the relationships between Ae. columnaris and common wheat chromosomes. In this paper, we report the development of 57 wheat - Ae. columnaris introgressive lines covering 8 of the14 chromosomes of Aegilops. Based on substitution spectra of hybrids and the results of FISH analysis of the parental Ae. columnaris line with seven DNA probes, we have developed the genetic nomenclature of the Uc and Xc chromosomes. Genetic groups and genome affinities were established for 11 of 14 chromosomes; the classification of the remaining three chromosomes remains unsolved. Each Ae. columnaris chromosome was characterized on the basis of C-banding pattern and the distribution of seven DNA sequences. Introgression processes were shown to depend on the parental wheat genotype and the level of divergence of homoeologous chromosomes. We found that lines carrying chromosome 5Xc are resistant to leaf rust; therefore, this chromosome could possess novel resistance genes that have never been utilized in wheat breeding. SN - 1480-3321 UR - https://www.unboundmedicine.com/medline/citation/29216443/Genetic_classification_of_Aegilops_columnaris_Zhuk___2n=4x=28_UcUcXcXc__chromosomes_based_on_FISH_analysis_and_substitution_patterns_in_common_wheat_×_Ae__columnaris_introgressive_lines_ L2 - https://cdnsciencepub.com/doi/10.1139/gen-2017-0186?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -