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Metabolism of hydrogen sulfide (H2S) and Production of Reactive Sulfur Species (RSS) by superoxide dismutase.
Redox Biol. 2018 05; 15:74-85.RB

Abstract

Reactive sulfur species (RSS) such as H2S, HS•, H2Sn, (n = 2-7) and HS2•- are chemically similar to H2O and the reactive oxygen species (ROS) HO•, H2O2, O2•- and act on common biological effectors. RSS were present in evolution long before ROS, and because both are metabolized by catalase it has been suggested that "antioxidant" enzymes originally evolved to regulate RSS and may continue to do so today. Here we examined RSS metabolism by Cu/Zn superoxide dismutase (SOD) using amperometric electrodes for dissolved H2S, a polysulfide-specific fluorescent probe (SSP4), and mass spectrometry to identify specific polysulfides (H2S2-H2S5). H2S was concentration- and oxygen-dependently oxidized by 1μM SOD to polysulfides (mainly H2S2, and to a lesser extent H2S3 and H2S5) with an EC50 of approximately 380μM H2S. H2S concentrations > 750μM inhibited SOD oxidation (IC50 = 1.25mM) with complete inhibition when H2S > 1.75mM. Polysulfides were not metabolized by SOD. SOD oxidation preferred dissolved H2S over hydrosulfide anion (HS-), whereas HS- inhibited polysulfide production. In hypoxia, other possible electron donors such as nitrate, nitrite, sulfite, sulfate, thiosulfate and metabisulfite were ineffective. Manganese SOD also catalyzed H2S oxidation to form polysulfides, but did not metabolize polysulfides indicating common attributes of these SODs. These experiments suggest that, unlike the well-known SOD-mediated dismutation of two O2•- to form H2O2 and O2, SOD catalyzes a reaction using H2S and O2 to form persulfide. These can then combine in various ways to form polysulfides and sulfur oxides. It is also possible that H2S (or polysulfides) interact/react with SOD cysteines to affect catalytic activity or to directly contribute to sulfide metabolism. Our studies suggest that H2S metabolism by SOD may have been an ancient mechanism to detoxify sulfide or to regulate RSS and along with catalase may continue to do so in contemporary organisms.

Authors+Show Affiliations

Indiana University School of Medicine - South Bend Center, South Bend, IN 46617, USA. Electronic address: olson.1@nd.edu.Indiana University School of Medicine - South Bend Center, South Bend, IN 46617, USA.Indiana University School of Medicine - South Bend Center, South Bend, IN 46617, USA.Indiana University School of Medicine - South Bend Center, South Bend, IN 46617, USA.Indiana University School of Medicine - South Bend Center, South Bend, IN 46617, USA.Indiana University School of Medicine - South Bend Center, South Bend, IN 46617, USA; University of Notre Dame, Notre Dame, IN 46556, USA.NIHR Southampton Biomedical Research Center, University of Southampton, Southampton, General Hospital, Southampton SO16 6YD, UK; Clinical & Experimental Sciences, Faculty of Medicine, Southampton General Hospital and Institute for Life Sciences, University of Southampton, Southampton SO16 6YD, UK.NIHR Southampton Biomedical Research Center, University of Southampton, Southampton, General Hospital, Southampton SO16 6YD, UK; Clinical & Experimental Sciences, Faculty of Medicine, Southampton General Hospital and Institute for Life Sciences, University of Southampton, Southampton SO16 6YD, UK.Cardiovascular Research Laboratory, Department of Cardiology, Pneumology and Angiology,Medical Faculty, Heinrich Heine University of Düsseldorf, Universitätstrasse 1, 40225 Düsseldorf, Germany.Central Arkansas Veteran's Healthcare System, Little Rock, AR 72205 USA; Departments of Medicine and Biochemistry, University of Arkansas for Medical Sciences, Little Rock, AR 72202 USA.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29220697

Citation

Olson, Kenneth R., et al. "Metabolism of Hydrogen Sulfide (H2S) and Production of Reactive Sulfur Species (RSS) By Superoxide Dismutase." Redox Biology, vol. 15, 2018, pp. 74-85.
Olson KR, Gao Y, Arif F, et al. Metabolism of hydrogen sulfide (H2S) and Production of Reactive Sulfur Species (RSS) by superoxide dismutase. Redox Biol. 2018;15:74-85.
Olson, K. R., Gao, Y., Arif, F., Arora, K., Patel, S., DeLeon, E. R., Sutton, T. R., Feelisch, M., Cortese-Krott, M. M., & Straub, K. D. (2018). Metabolism of hydrogen sulfide (H2S) and Production of Reactive Sulfur Species (RSS) by superoxide dismutase. Redox Biology, 15, 74-85. https://doi.org/10.1016/j.redox.2017.11.009
Olson KR, et al. Metabolism of Hydrogen Sulfide (H2S) and Production of Reactive Sulfur Species (RSS) By Superoxide Dismutase. Redox Biol. 2018;15:74-85. PubMed PMID: 29220697.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolism of hydrogen sulfide (H2S) and Production of Reactive Sulfur Species (RSS) by superoxide dismutase. AU - Olson,Kenneth R, AU - Gao,Yan, AU - Arif,Faihaan, AU - Arora,Kanika, AU - Patel,Shivali, AU - DeLeon,Eric R, AU - Sutton,Thomas R, AU - Feelisch,Martin, AU - Cortese-Krott,Miriam M, AU - Straub,Karl D, Y1 - 2017/11/20/ PY - 2017/09/06/received PY - 2017/10/16/revised PY - 2017/11/08/accepted PY - 2017/12/9/pubmed PY - 2018/10/13/medline PY - 2017/12/9/entrez KW - Antioxidants KW - Hydrogen peroxide KW - Oxidants KW - Reactive Species Interactome KW - Reactive oxygen species KW - Redox KW - Superoxide SP - 74 EP - 85 JF - Redox biology JO - Redox Biol VL - 15 N2 - Reactive sulfur species (RSS) such as H2S, HS•, H2Sn, (n = 2-7) and HS2•- are chemically similar to H2O and the reactive oxygen species (ROS) HO•, H2O2, O2•- and act on common biological effectors. RSS were present in evolution long before ROS, and because both are metabolized by catalase it has been suggested that "antioxidant" enzymes originally evolved to regulate RSS and may continue to do so today. Here we examined RSS metabolism by Cu/Zn superoxide dismutase (SOD) using amperometric electrodes for dissolved H2S, a polysulfide-specific fluorescent probe (SSP4), and mass spectrometry to identify specific polysulfides (H2S2-H2S5). H2S was concentration- and oxygen-dependently oxidized by 1μM SOD to polysulfides (mainly H2S2, and to a lesser extent H2S3 and H2S5) with an EC50 of approximately 380μM H2S. H2S concentrations > 750μM inhibited SOD oxidation (IC50 = 1.25mM) with complete inhibition when H2S > 1.75mM. Polysulfides were not metabolized by SOD. SOD oxidation preferred dissolved H2S over hydrosulfide anion (HS-), whereas HS- inhibited polysulfide production. In hypoxia, other possible electron donors such as nitrate, nitrite, sulfite, sulfate, thiosulfate and metabisulfite were ineffective. Manganese SOD also catalyzed H2S oxidation to form polysulfides, but did not metabolize polysulfides indicating common attributes of these SODs. These experiments suggest that, unlike the well-known SOD-mediated dismutation of two O2•- to form H2O2 and O2, SOD catalyzes a reaction using H2S and O2 to form persulfide. These can then combine in various ways to form polysulfides and sulfur oxides. It is also possible that H2S (or polysulfides) interact/react with SOD cysteines to affect catalytic activity or to directly contribute to sulfide metabolism. Our studies suggest that H2S metabolism by SOD may have been an ancient mechanism to detoxify sulfide or to regulate RSS and along with catalase may continue to do so in contemporary organisms. SN - 2213-2317 UR - https://www.unboundmedicine.com/medline/citation/29220697/Metabolism_of_hydrogen_sulfide__H2S__and_Production_of_Reactive_Sulfur_Species__RSS__by_superoxide_dismutase_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S2213-2317(17)30673-0 DB - PRIME DP - Unbound Medicine ER -