Tags

Type your tag names separated by a space and hit enter

White butterfly (Clerodendrum volubile) leaf extract protects against carbon tetrachloride-induced hepatotoxicity in rats.
Biomed Pharmacother 2017; 96:924-929BP

Abstract

Currently, there is increasing attention towards flavonoids and phenolic compounds of plant origin because of their association with decrease in the incidence of cardiovascular diseases and different types of cancer. The present study investigates the protective effect of Clerodendrum volubile (C. volubile) methanolic extracts against carbon tetrachloride (CCl4)-induced hepatotoxicity in rats. Control rats (group I) received olive oil (1 mL/kg, i.p.), group II received CCl4 in olive oil (1 ml/kg, i.p.) to induce hepatotoxicity, groups III, IV and V were pretreated with leaf extract of C. volubile at 125 mg/kg, 250 mg/kg and 500 mg/kg body weight respectively for 14 days prior to CCl4 administration, group VI received vitamin E (100 mg/kg, p.o.) as standard antioxidant to compare with antioxidant effects of the extract. CCl4 hepatotoxicity, characterized by significant (P < 0.05) increase in the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP), hepatic degeneration, and inflammation was attenuated by C. volubile methanolic extracts. The serum lipid parameters which include high density lipoprotein (HDL) and low density lipoprotein (LDL) were significantly (P < 0.05) decreased, and increased respectively by CCl4. Methanolic extracts of C. volubile significantly prevented the decrease in the level of HDL and the increase in LDL in a dose-dependent manner (P < 0.05). Decrease in total protein induced by CCl4 was moderately increased following administration of methanolic extracts of C. volubile. Lipid peroxidation was significantly (P < 0.05) reduced while the reduced glutathione (GSH) level and the activities of hepatic antioxidant enzymes (catalase, superoxide dismutase, and glutathione peroxidase) were significantly elevated by C. volubile extract in the CCl4-treated rats. Our findings indicate that C. volubile extract has a significant protective effect against CCl4-induced hepatotoxicity in rats which may be due to its antioxidant properties which is comparable to the reference antioxidant, vitamin E, used in this study.

Authors+Show Affiliations

Department of Biochemistry, Faculty of Science, Ekiti State University, Ado-Ekiti. P.M.B. 5363, Ado-Ekiti, Nigeria. Electronic address: olorunfemi.molehin@eksu.edu.ng.Department of Biochemistry, Faculty of Science, Ekiti State University, Ado-Ekiti. P.M.B. 5363, Ado-Ekiti, Nigeria.Department of Medical Biochemistry, College of Medicine, Ekiti State University, Ado-Ekiti. P.M.B. 5363, Ado-Ekiti, Nigeria.Department of Biological Sciences, McPherson University, Seriki Sotayo, Ajebo, Ogun State, Nigeria.Department of Biochemistry, Faculty of Science, Ekiti State University, Ado-Ekiti. P.M.B. 5363, Ado-Ekiti, Nigeria.Department of Biochemistry, Faculty of Science, Ekiti State University, Ado-Ekiti. P.M.B. 5363, Ado-Ekiti, Nigeria.Department of Biochemistry, Faculty of Science, Ekiti State University, Ado-Ekiti. P.M.B. 5363, Ado-Ekiti, Nigeria.Department of Biochemistry, Faculty of Science, Ekiti State University, Ado-Ekiti. P.M.B. 5363, Ado-Ekiti, Nigeria.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29224792

Citation

Molehin, Olorunfemi R., et al. "White Butterfly (Clerodendrum Volubile) Leaf Extract Protects Against Carbon Tetrachloride-induced Hepatotoxicity in Rats." Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, vol. 96, 2017, pp. 924-929.
Molehin OR, Oloyede OI, Idowu KA, et al. White butterfly (Clerodendrum volubile) leaf extract protects against carbon tetrachloride-induced hepatotoxicity in rats. Biomed Pharmacother. 2017;96:924-929.
Molehin, O. R., Oloyede, O. I., Idowu, K. A., Adeyanju, A. A., Olowoyeye, A. O., Tubi, O. I., ... Gold, A. S. (2017). White butterfly (Clerodendrum volubile) leaf extract protects against carbon tetrachloride-induced hepatotoxicity in rats. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 96, pp. 924-929. doi:10.1016/j.biopha.2017.12.005.
Molehin OR, et al. White Butterfly (Clerodendrum Volubile) Leaf Extract Protects Against Carbon Tetrachloride-induced Hepatotoxicity in Rats. Biomed Pharmacother. 2017;96:924-929. PubMed PMID: 29224792.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - White butterfly (Clerodendrum volubile) leaf extract protects against carbon tetrachloride-induced hepatotoxicity in rats. AU - Molehin,Olorunfemi R, AU - Oloyede,Omotade I, AU - Idowu,Kehinde A, AU - Adeyanju,Anne A, AU - Olowoyeye,Ayorinde O, AU - Tubi,Olusegun I, AU - Komolafe,Olayemi E, AU - Gold,Aanuoluwapo S, Y1 - 2017/12/07/ PY - 2017/09/04/received PY - 2017/11/30/revised PY - 2017/12/01/accepted PY - 2017/12/12/pubmed PY - 2018/7/27/medline PY - 2017/12/12/entrez KW - Antioxidant KW - Carbon tetrachloride KW - Clerodendrum volubile KW - Hepatotoxicity KW - Histopathology KW - Oxidative stress SP - 924 EP - 929 JF - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JO - Biomed. Pharmacother. VL - 96 N2 - Currently, there is increasing attention towards flavonoids and phenolic compounds of plant origin because of their association with decrease in the incidence of cardiovascular diseases and different types of cancer. The present study investigates the protective effect of Clerodendrum volubile (C. volubile) methanolic extracts against carbon tetrachloride (CCl4)-induced hepatotoxicity in rats. Control rats (group I) received olive oil (1 mL/kg, i.p.), group II received CCl4 in olive oil (1 ml/kg, i.p.) to induce hepatotoxicity, groups III, IV and V were pretreated with leaf extract of C. volubile at 125 mg/kg, 250 mg/kg and 500 mg/kg body weight respectively for 14 days prior to CCl4 administration, group VI received vitamin E (100 mg/kg, p.o.) as standard antioxidant to compare with antioxidant effects of the extract. CCl4 hepatotoxicity, characterized by significant (P < 0.05) increase in the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP), hepatic degeneration, and inflammation was attenuated by C. volubile methanolic extracts. The serum lipid parameters which include high density lipoprotein (HDL) and low density lipoprotein (LDL) were significantly (P < 0.05) decreased, and increased respectively by CCl4. Methanolic extracts of C. volubile significantly prevented the decrease in the level of HDL and the increase in LDL in a dose-dependent manner (P < 0.05). Decrease in total protein induced by CCl4 was moderately increased following administration of methanolic extracts of C. volubile. Lipid peroxidation was significantly (P < 0.05) reduced while the reduced glutathione (GSH) level and the activities of hepatic antioxidant enzymes (catalase, superoxide dismutase, and glutathione peroxidase) were significantly elevated by C. volubile extract in the CCl4-treated rats. Our findings indicate that C. volubile extract has a significant protective effect against CCl4-induced hepatotoxicity in rats which may be due to its antioxidant properties which is comparable to the reference antioxidant, vitamin E, used in this study. SN - 1950-6007 UR - https://www.unboundmedicine.com/medline/citation/29224792/White_butterfly__Clerodendrum_volubile__leaf_extract_protects_against_carbon_tetrachloride_induced_hepatotoxicity_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0753-3322(17)34560-2 DB - PRIME DP - Unbound Medicine ER -