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Temporal ELISA response of rams to Brucella ovis following experimental infection or vaccination.
Res Vet Sci. 1989 Jan; 46(1):73-8.RV

Abstract

Sera from rams vaccinated with antigens extracted chaotropically from Brucella ovis by potassium thiocyanate treatment were used to optimise a whole-cell, enzyme-linked immunosorbent assay (CELISA) and to monitor the temporal serological response of rams which had been challenged with infected semen by the intranasal or intrapreputial route. Three patterns of CELISA response were detected. Thirteen of 15 rams intranasally challenged did not respond serologically (pattern 1 or nil response). Only one of 15 rams in the intranasal group exhibited a rise and fall response with CELISA (pattern 2), while another showed a rise and surge response (pattern 3). The numbers of rams in the intrapreputial group which displayed a pattern 1 or 2 or 3 response were four, nine and two, respectively. No ram with a pattern 2 response excreted B ovis in the semen or showed any other evidence of infection, whereas rams with a pattern 3 response excreted B ovis in the semen and developed palpable lesions. Intrapreputially challenged rams that were CELISA-positive consistently mounted an antibody response against B ovis about two to four weeks earlier than intranasally challenged rams.

Authors+Show Affiliations

Central Veterinary Laboratory, Department of Agriculture, New South Wales, Glenfield, Australia.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2922509

Citation

Chin, J C., and J W. Plant. "Temporal ELISA Response of Rams to Brucella Ovis Following Experimental Infection or Vaccination." Research in Veterinary Science, vol. 46, no. 1, 1989, pp. 73-8.
Chin JC, Plant JW. Temporal ELISA response of rams to Brucella ovis following experimental infection or vaccination. Res Vet Sci. 1989;46(1):73-8.
Chin, J. C., & Plant, J. W. (1989). Temporal ELISA response of rams to Brucella ovis following experimental infection or vaccination. Research in Veterinary Science, 46(1), 73-8.
Chin JC, Plant JW. Temporal ELISA Response of Rams to Brucella Ovis Following Experimental Infection or Vaccination. Res Vet Sci. 1989;46(1):73-8. PubMed PMID: 2922509.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Temporal ELISA response of rams to Brucella ovis following experimental infection or vaccination. AU - Chin,J C, AU - Plant,J W, PY - 1989/1/1/pubmed PY - 1989/1/1/medline PY - 1989/1/1/entrez SP - 73 EP - 8 JF - Research in veterinary science JO - Res. Vet. Sci. VL - 46 IS - 1 N2 - Sera from rams vaccinated with antigens extracted chaotropically from Brucella ovis by potassium thiocyanate treatment were used to optimise a whole-cell, enzyme-linked immunosorbent assay (CELISA) and to monitor the temporal serological response of rams which had been challenged with infected semen by the intranasal or intrapreputial route. Three patterns of CELISA response were detected. Thirteen of 15 rams intranasally challenged did not respond serologically (pattern 1 or nil response). Only one of 15 rams in the intranasal group exhibited a rise and fall response with CELISA (pattern 2), while another showed a rise and surge response (pattern 3). The numbers of rams in the intrapreputial group which displayed a pattern 1 or 2 or 3 response were four, nine and two, respectively. No ram with a pattern 2 response excreted B ovis in the semen or showed any other evidence of infection, whereas rams with a pattern 3 response excreted B ovis in the semen and developed palpable lesions. Intrapreputially challenged rams that were CELISA-positive consistently mounted an antibody response against B ovis about two to four weeks earlier than intranasally challenged rams. SN - 0034-5288 UR - https://www.unboundmedicine.com/medline/citation/2922509/Temporal_ELISA_response_of_rams_to_Brucella_ovis_following_experimental_infection_or_vaccination_ DB - PRIME DP - Unbound Medicine ER -