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[Effects of electroacupuncture on the activities of gliocytes of cervical spinal cord in rats with thyroid incisional pain].
Zhongguo Zhen Jiu. 2016 Jul 12; 36(7):727-733.ZZ

Abstract

OBJECTIVE

To observe the impacts of electroacupuncture (EA) on microgliacytes and astrocytes of cervical spinal cord in rats with thyroid incisional pain and explore the mechanism of acupuncture anesthesia in thyroid surgery.

METHODS

Sixty Wistar male rats were randomized into a normal group, a model group, a Futu (LI 18) group, a Hegu (LI 4)-Neiguan (PC 6) group and a Zusanli (ST 36)-Yanglingquan (GB 34) group, 12 rats in each one. Except the normal group, a longitudinal incision, about 1.5 cm in length was done along the neck midline in the rats of the rest groups to prepare the model of thyroid incisional pain. In the Futu (LI 18) group, the Hegu (LI 4)-Neiguan (PC 6) group and the Zusanli (ST 36)-Yanglingquan (GB 34) group, after modeling for 4 h, 24 h and 48 h, EA was applied to bilateral "Futu" (LI 18), "Hegu" (LI 4) "Neiguan" (PC 6) and"Zusanli" (ST 36) "Yanglingquan" (GB 34) separately, once a day, continuously for 3 days. In the normal group and the model group, no any intervention was applied. The thermal radiant apparatus was used to detect the thermal pain threshold (PT). The fluorescence quantitative RT-PCR and the Western blotting (WB) were used to determine the expressions of protein and gene of microglia activation markers Iba1 and CD11b and the astrocyte specific protein marker, glial fibrillary acid protein (GFAP) in cervical spinal cord (C2 to C6) after intervention in the rats of each group.

RESULTS

After intervention, as compared with the normal group, in the model group, the neck PT was reduced apparently (P<0.05), the expressions of Iba1 and CD11b and GFAP mRNA as well as the protein expressions in the spinal cord of C2 to C6 were up-regulated apparently (P<0.05, P<0.01). As compared with the model group, in the Futu (LI 18) and the Hegu (LI 4)-Neiguan (PC 6) group, PT was increased significantly (both P<0.05) and that did not change apparently in the Zusanli (ST 36)-Yanglingquan (GB 34) group (P>0.05). In the Futu (LI 18) group, the protein and gene expressions of Iba1, CD11b and GFAP were lower than those in the model group (all P<0.05). In the Hegu (LI 4)-Neiguan (PC 6) group, the expressions of Iba1 mRNA, CD11b protein, GFAP mRNA and protein were all lower apparently than those in the model group (all P<0.05). In the Zusanli (ST 36)-Yanglingquan (GB 34) group, the expressions of Iba1, CD11b and GFAP proteins were not different significantly as compared with the model group (all P>0.05). In the Zusanli (ST 36)-Yanglingquan (GB 34) group, the expressions of Iba1 mRNA and CD11b mRNA and protein expressions in the spinal cord of C2 to C6 were higher apparently than those in the Futu (LI 18) group (P<0.01, P<0.05); the expressions of Iba1 mRNA and CD11b protein expressions were higher than those in the Hegu (LI 4)-Neiguan (PC 6) group (all P<0.05); GFAP mRNA and protein expressions were higher apparently than those in the Futu (LI 18) group and the Hegu (LI 4)-Neiguan (PC 6) group (all P<0.05).

CONCLUSIONS

EA at "Futu" (LI 18) or "Hegu" (LI 4), "Neiguan" (PC 6) relieves the acute neck incisional pain in the rats and its effect may be closely relevant with the down-regulation of the activities of microgliacytes and astrocytes in the spinal cords.

Authors+Show Affiliations

Research Room of Biochemistry and Molecular Biology, China Academy of Chinese Medical Sciences, Beijing 100700, China.Research Room of Biochemistry and Molecular Biology, China Academy of Chinese Medical Sciences, Beijing 100700, China.Research Room of Physiologic Function, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.Research Room of Physiologic Function, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.Research Room of Physiologic Function, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.Research Room of Physiologic Function, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

29231413

Citation

Yang, Yongsheng, et al. "[Effects of Electroacupuncture On the Activities of Gliocytes of Cervical Spinal Cord in Rats With Thyroid Incisional Pain]." Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, vol. 36, no. 7, 2016, pp. 727-733.
Yang Y, Qiao L, Wang J, et al. [Effects of electroacupuncture on the activities of gliocytes of cervical spinal cord in rats with thyroid incisional pain]. Zhongguo Zhen Jiu. 2016;36(7):727-733.
Yang, Y., Qiao, L., Wang, J., Chen, S., Tan, L., & Liu, J. (2016). [Effects of electroacupuncture on the activities of gliocytes of cervical spinal cord in rats with thyroid incisional pain]. Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, 36(7), 727-733. https://doi.org/10.13703/j.0255-2930.2016.07.014
Yang Y, et al. [Effects of Electroacupuncture On the Activities of Gliocytes of Cervical Spinal Cord in Rats With Thyroid Incisional Pain]. Zhongguo Zhen Jiu. 2016 Jul 12;36(7):727-733. PubMed PMID: 29231413.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of electroacupuncture on the activities of gliocytes of cervical spinal cord in rats with thyroid incisional pain]. AU - Yang,Yongsheng, AU - Qiao,Lina, AU - Wang,Junying, AU - Chen,Shuping, AU - Tan,Lianhong, AU - Liu,Junling, PY - 2017/12/13/entrez PY - 2016/7/12/pubmed PY - 2018/6/14/medline KW - CD11b KW - GFAP KW - Iba1 KW - acupuncture KW - anesthesia KW - astrocytes KW - microgliacytes KW - spinal cord KW - thyroid incisional pain SP - 727 EP - 733 JF - Zhongguo zhen jiu = Chinese acupuncture & moxibustion JO - Zhongguo Zhen Jiu VL - 36 IS - 7 N2 - OBJECTIVE: To observe the impacts of electroacupuncture (EA) on microgliacytes and astrocytes of cervical spinal cord in rats with thyroid incisional pain and explore the mechanism of acupuncture anesthesia in thyroid surgery. METHODS: Sixty Wistar male rats were randomized into a normal group, a model group, a Futu (LI 18) group, a Hegu (LI 4)-Neiguan (PC 6) group and a Zusanli (ST 36)-Yanglingquan (GB 34) group, 12 rats in each one. Except the normal group, a longitudinal incision, about 1.5 cm in length was done along the neck midline in the rats of the rest groups to prepare the model of thyroid incisional pain. In the Futu (LI 18) group, the Hegu (LI 4)-Neiguan (PC 6) group and the Zusanli (ST 36)-Yanglingquan (GB 34) group, after modeling for 4 h, 24 h and 48 h, EA was applied to bilateral "Futu" (LI 18), "Hegu" (LI 4) "Neiguan" (PC 6) and"Zusanli" (ST 36) "Yanglingquan" (GB 34) separately, once a day, continuously for 3 days. In the normal group and the model group, no any intervention was applied. The thermal radiant apparatus was used to detect the thermal pain threshold (PT). The fluorescence quantitative RT-PCR and the Western blotting (WB) were used to determine the expressions of protein and gene of microglia activation markers Iba1 and CD11b and the astrocyte specific protein marker, glial fibrillary acid protein (GFAP) in cervical spinal cord (C2 to C6) after intervention in the rats of each group. RESULTS: After intervention, as compared with the normal group, in the model group, the neck PT was reduced apparently (P<0.05), the expressions of Iba1 and CD11b and GFAP mRNA as well as the protein expressions in the spinal cord of C2 to C6 were up-regulated apparently (P<0.05, P<0.01). As compared with the model group, in the Futu (LI 18) and the Hegu (LI 4)-Neiguan (PC 6) group, PT was increased significantly (both P<0.05) and that did not change apparently in the Zusanli (ST 36)-Yanglingquan (GB 34) group (P>0.05). In the Futu (LI 18) group, the protein and gene expressions of Iba1, CD11b and GFAP were lower than those in the model group (all P<0.05). In the Hegu (LI 4)-Neiguan (PC 6) group, the expressions of Iba1 mRNA, CD11b protein, GFAP mRNA and protein were all lower apparently than those in the model group (all P<0.05). In the Zusanli (ST 36)-Yanglingquan (GB 34) group, the expressions of Iba1, CD11b and GFAP proteins were not different significantly as compared with the model group (all P>0.05). In the Zusanli (ST 36)-Yanglingquan (GB 34) group, the expressions of Iba1 mRNA and CD11b mRNA and protein expressions in the spinal cord of C2 to C6 were higher apparently than those in the Futu (LI 18) group (P<0.01, P<0.05); the expressions of Iba1 mRNA and CD11b protein expressions were higher than those in the Hegu (LI 4)-Neiguan (PC 6) group (all P<0.05); GFAP mRNA and protein expressions were higher apparently than those in the Futu (LI 18) group and the Hegu (LI 4)-Neiguan (PC 6) group (all P<0.05). CONCLUSIONS: EA at "Futu" (LI 18) or "Hegu" (LI 4), "Neiguan" (PC 6) relieves the acute neck incisional pain in the rats and its effect may be closely relevant with the down-regulation of the activities of microgliacytes and astrocytes in the spinal cords. SN - 0255-2930 UR - https://www.unboundmedicine.com/medline/citation/29231413/[Effects_of_electroacupuncture_on_the_activities_of_gliocytes_of_cervical_spinal_cord_in_rats_with_thyroid_incisional_pain]_ DB - PRIME DP - Unbound Medicine ER -