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[Effect of acupuncture treatment on gene expression of neurotransmitters of brain tissue in rats with post-stroke depression].
Zhongguo Zhen Jiu. 2017 Jun 12; 37(6):637-641.ZZ

Abstract

OBJECTIVE

To observe the effects of acupuncture on mRNA of the neurotransmitter serotonin transporter (5-HTT), 5-HT1Areceptor (5-HT1AR), norepinephrineα2 receptor (NEα2R) in brain tissue in rats with post-stroke depression.

METHODS

Forty male SD rats were randomly divided into a normal group, a model group, a medication group and an acupuncture group, 10 rats in each one. The model of post-stroke depression was established by occlusion of middle cerebral artery and chronic unpredictable mild stress method. After model was successfully established, the rats in the normal group and model group received no treatment; the rats in medication group were treated with intragastric administration of fluoxetine (2 mg/kg); the rats in acupuncture group were treated with acupuncture at "Baihui" (GV 20), "Fengfu" (GV 16), "Shenmen" (HT 7) and "Taichong" (LR 3) for 20 min, during which manual stimulation was given once, once a day, 7 days as one course, and totally 3 courses were given with an interval of one day between courses. The changes of rat behavior and 5-HT, NE were observed after intervention; the RT-PCR method was applied to observe the mRNA of 5-HTT, 5-HT1AR, NEα2R in hippocampus, raphe nucleus and locus coeruleus.

RESULTS

After treatment, compared with the normal group, the Zea Longa score in the model group was increased, while sugar water consumption, the number of horizontal and vertical movement of open-field test was reduced (all P<0.01); compared with the model group, the Zea Longa score in the medication group and acupuncture group was reduced, while sugar water consumption, the number of horizontal and vertical movement of open-field test were increased (P<0.01, P<0.05); compared between the medication group and acupuncture group, the behavior changes were not significantly different (all P>0.05). After treatment, compared with the normal group, the content of 5-HT and NE in brain tissue and mRNA expression of 5-HTT, 5-HT1AR in hippocampus, raphe nucleus and locus coeruleus in the model group were reduced (all P<0.01), but the mRNA expression of NEα2R was increased (P<0.01);compared with the model group, the content of 5-HT and NE in brain tissue and mRNA expression of 5-HTT, 5-HT1AR in hippocampus, raphe nucleus and locus coeruleus in the medication group and acupuncture group were increased (P<0.01, P<0.05), while the mRNA expression of NEα2R was reduced (all P<0.01). The differences between medication group and acupuncture group were not significantly different (all P>0.05).

CONCLUSIONS

Acupuncture could significantly improve behavioral change in rats with post-stroke depression, which may be related to the regulation of 5-HT, NE in cerebral cortex as well as mRNA expressions of 5-HTT, 5-HT1AR, NEα2R in hippocampus, raphe nucleus and locus coeruleus.

Authors+Show Affiliations

The Second Affiliated Hospital, Anhui University of CM, Hefei 230061, China.The Second Affiliated Hospital, Anhui University of CM, Hefei 230061, China.The Second Affiliated Hospital, Anhui University of CM, Hefei 230061, China.Anhui University of CM.The Second Affiliated Hospital, Anhui University of CM, Hefei 230061, China.Anhui University of CM.The Second Affiliated Hospital, Anhui University of CM, Hefei 230061, China.The Second Affiliated Hospital, Anhui University of CM, Hefei 230061, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

29231507

Citation

Xiao, Wei, et al. "[Effect of Acupuncture Treatment On Gene Expression of Neurotransmitters of Brain Tissue in Rats With Post-stroke Depression]." Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, vol. 37, no. 6, 2017, pp. 637-641.
Xiao W, Zhang X, Wang Z, et al. [Effect of acupuncture treatment on gene expression of neurotransmitters of brain tissue in rats with post-stroke depression]. Zhongguo Zhen Jiu. 2017;37(6):637-641.
Xiao, W., Zhang, X., Wang, Z., Wang, Y., Guo, X., He, L., Liang, F., & Hu, H. (2017). [Effect of acupuncture treatment on gene expression of neurotransmitters of brain tissue in rats with post-stroke depression]. Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, 37(6), 637-641. https://doi.org/10.13703/j.0255-2930.2017.06.017
Xiao W, et al. [Effect of Acupuncture Treatment On Gene Expression of Neurotransmitters of Brain Tissue in Rats With Post-stroke Depression]. Zhongguo Zhen Jiu. 2017 Jun 12;37(6):637-641. PubMed PMID: 29231507.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of acupuncture treatment on gene expression of neurotransmitters of brain tissue in rats with post-stroke depression]. AU - Xiao,Wei, AU - Zhang,Xianbao, AU - Wang,Zhen, AU - Wang,Yan, AU - Guo,Xiaoli, AU - He,Ling, AU - Liang,Fajun, AU - Hu,Huixing, PY - 2017/12/13/entrez PY - 2017/12/13/pubmed PY - 2018/6/28/medline KW - acupuncture KW - neurotransmitters KW - post-stroke depression KW - serotonin transporter (5-HTT), serotonin 1A receptor (5-HT1AR), norepinephrineα2 receptor (NEα2R) SP - 637 EP - 641 JF - Zhongguo zhen jiu = Chinese acupuncture & moxibustion JO - Zhongguo Zhen Jiu VL - 37 IS - 6 N2 - OBJECTIVE: To observe the effects of acupuncture on mRNA of the neurotransmitter serotonin transporter (5-HTT), 5-HT1Areceptor (5-HT1AR), norepinephrineα2 receptor (NEα2R) in brain tissue in rats with post-stroke depression. METHODS: Forty male SD rats were randomly divided into a normal group, a model group, a medication group and an acupuncture group, 10 rats in each one. The model of post-stroke depression was established by occlusion of middle cerebral artery and chronic unpredictable mild stress method. After model was successfully established, the rats in the normal group and model group received no treatment; the rats in medication group were treated with intragastric administration of fluoxetine (2 mg/kg); the rats in acupuncture group were treated with acupuncture at "Baihui" (GV 20), "Fengfu" (GV 16), "Shenmen" (HT 7) and "Taichong" (LR 3) for 20 min, during which manual stimulation was given once, once a day, 7 days as one course, and totally 3 courses were given with an interval of one day between courses. The changes of rat behavior and 5-HT, NE were observed after intervention; the RT-PCR method was applied to observe the mRNA of 5-HTT, 5-HT1AR, NEα2R in hippocampus, raphe nucleus and locus coeruleus. RESULTS: After treatment, compared with the normal group, the Zea Longa score in the model group was increased, while sugar water consumption, the number of horizontal and vertical movement of open-field test was reduced (all P<0.01); compared with the model group, the Zea Longa score in the medication group and acupuncture group was reduced, while sugar water consumption, the number of horizontal and vertical movement of open-field test were increased (P<0.01, P<0.05); compared between the medication group and acupuncture group, the behavior changes were not significantly different (all P>0.05). After treatment, compared with the normal group, the content of 5-HT and NE in brain tissue and mRNA expression of 5-HTT, 5-HT1AR in hippocampus, raphe nucleus and locus coeruleus in the model group were reduced (all P<0.01), but the mRNA expression of NEα2R was increased (P<0.01);compared with the model group, the content of 5-HT and NE in brain tissue and mRNA expression of 5-HTT, 5-HT1AR in hippocampus, raphe nucleus and locus coeruleus in the medication group and acupuncture group were increased (P<0.01, P<0.05), while the mRNA expression of NEα2R was reduced (all P<0.01). The differences between medication group and acupuncture group were not significantly different (all P>0.05). CONCLUSIONS: Acupuncture could significantly improve behavioral change in rats with post-stroke depression, which may be related to the regulation of 5-HT, NE in cerebral cortex as well as mRNA expressions of 5-HTT, 5-HT1AR, NEα2R in hippocampus, raphe nucleus and locus coeruleus. SN - 0255-2930 UR - https://www.unboundmedicine.com/medline/citation/29231507/[Effect_of_acupuncture_treatment_on_gene_expression_of_neurotransmitters_of_brain_tissue_in_rats_with_post_stroke_depression]_ L2 - https://medlineplus.gov/depression.html DB - PRIME DP - Unbound Medicine ER -