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Molecular characterization, tissue distribution and differential nutritional regulation of putative Elovl5 elongase in silver barb (Puntius gonionotus).

Abstract

Fatty acid desaturase (Fads) and elongase (Elovl) are two important enzymes involved in the biosynthesis of long-chain polyunsaturated fatty acids (LC-PUFAs) in fishes. Silver barb (Puntius gonionotus) is considered a promising medium carp species for freshwater aquaculture in Asia. Earlier, Δ6 fads cDNA was characterized to understand the molecular mechanism of LC-PUFA biosynthesis in this species. In the present study elovl5-like elongase full-length cDNA was cloned and characterized by RACE (rapid amplification of cDNA ends), and analyzed its basal expression in various organs/tissues by quantitative real time PCR (qRT-PCR) assay. Differential expression of elovl5 cDNA in various tissues following replacement of fish oil (FO) with linseed oil (LO) in the diet also studied. The full length cDNA sequence of silver barb elovl5-like elongase consisted of 1853bp, including an open reading frame (ORF) of 876bp encoding a precursor protein of 291 amino acids possessing the entire characteristic features of Elovl protein. The elovl5 transcript was constitutively expressed in all the tissues examined: muscle, liver, brain, kidney, skin, intestine, gill and eye. The expression of elovl5 gene was upregulated, whereas the serum biochemical constituents were either remain unchanged or lowered following replacement of FO with LO in the diets. We report for the first time, an in silico 3D structure model of elongase protein from silver barb. All these findings may contribute to a better understanding the potential regulatory mechanisms involved and may be useful for enhancing endogenous LC-PUFA production in silver barb.

Authors+Show Affiliations

Division of Fish Nutrition and Physiology, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, India.Division of Fish Nutrition and Physiology, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, India.Division of Fish Nutrition and Physiology, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, India.Division of Fish Health Management, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, India.School of Biological Sciences, National Institute of Science Education & Research Bhubaneswar, Jatni, Khurda, Odisha, India.Division of Fish Nutrition and Physiology, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, India. Electronic address: Ashis.Saha@icar.gov.in.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29233754

Citation

Nayak, Madhusmita, et al. "Molecular Characterization, Tissue Distribution and Differential Nutritional Regulation of Putative Elovl5 Elongase in Silver Barb (Puntius Gonionotus)." Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, vol. 217, 2018, pp. 27-39.
Nayak M, Pradhan A, Giri SS, et al. Molecular characterization, tissue distribution and differential nutritional regulation of putative Elovl5 elongase in silver barb (Puntius gonionotus). Comp Biochem Physiol B, Biochem Mol Biol. 2018;217:27-39.
Nayak, M., Pradhan, A., Giri, S. S., Samanta, M., Konkimalla, V. B., & Saha, A. (2018). Molecular characterization, tissue distribution and differential nutritional regulation of putative Elovl5 elongase in silver barb (Puntius gonionotus). Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 217, 27-39. https://doi.org/10.1016/j.cbpb.2017.12.004
Nayak M, et al. Molecular Characterization, Tissue Distribution and Differential Nutritional Regulation of Putative Elovl5 Elongase in Silver Barb (Puntius Gonionotus). Comp Biochem Physiol B, Biochem Mol Biol. 2018;217:27-39. PubMed PMID: 29233754.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization, tissue distribution and differential nutritional regulation of putative Elovl5 elongase in silver barb (Puntius gonionotus). AU - Nayak,Madhusmita, AU - Pradhan,Avinash, AU - Giri,Shiba Shankar, AU - Samanta,Mrinal, AU - Konkimalla,V Badireenath, AU - Saha,Ashis, Y1 - 2017/12/09/ PY - 2017/09/22/received PY - 2017/12/05/revised PY - 2017/12/05/accepted PY - 2017/12/14/pubmed PY - 2018/11/9/medline PY - 2017/12/14/entrez KW - 3D structure KW - Elovl 5 KW - LC-PUFA biosynthesis KW - Nutritional regulation KW - Silver barb SP - 27 EP - 39 JF - Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology JO - Comp. Biochem. Physiol. B, Biochem. Mol. Biol. VL - 217 N2 - Fatty acid desaturase (Fads) and elongase (Elovl) are two important enzymes involved in the biosynthesis of long-chain polyunsaturated fatty acids (LC-PUFAs) in fishes. Silver barb (Puntius gonionotus) is considered a promising medium carp species for freshwater aquaculture in Asia. Earlier, Δ6 fads cDNA was characterized to understand the molecular mechanism of LC-PUFA biosynthesis in this species. In the present study elovl5-like elongase full-length cDNA was cloned and characterized by RACE (rapid amplification of cDNA ends), and analyzed its basal expression in various organs/tissues by quantitative real time PCR (qRT-PCR) assay. Differential expression of elovl5 cDNA in various tissues following replacement of fish oil (FO) with linseed oil (LO) in the diet also studied. The full length cDNA sequence of silver barb elovl5-like elongase consisted of 1853bp, including an open reading frame (ORF) of 876bp encoding a precursor protein of 291 amino acids possessing the entire characteristic features of Elovl protein. The elovl5 transcript was constitutively expressed in all the tissues examined: muscle, liver, brain, kidney, skin, intestine, gill and eye. The expression of elovl5 gene was upregulated, whereas the serum biochemical constituents were either remain unchanged or lowered following replacement of FO with LO in the diets. We report for the first time, an in silico 3D structure model of elongase protein from silver barb. All these findings may contribute to a better understanding the potential regulatory mechanisms involved and may be useful for enhancing endogenous LC-PUFA production in silver barb. SN - 1879-1107 UR - https://www.unboundmedicine.com/medline/citation/29233754/Molecular_characterization_tissue_distribution_and_differential_nutritional_regulation_of_putative_Elovl5_elongase_in_silver_barb__Puntius_gonionotus__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1096-4959(17)30193-8 DB - PRIME DP - Unbound Medicine ER -