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A Chinese Herbal Formula, Gengnianchun, Ameliorates β-Amyloid Peptide Toxicity in a Caenorhabditis elegans Model of Alzheimer's Disease.
Evid Based Complement Alternat Med. 2017; 2017:7480980.EB

Abstract

Alzheimer's disease (AD) is an age-related neurodegenerative disorder, and the few drugs that are currently available only treat the symptoms. Traditional medicine or phytotherapy has been shown to protect against AD. In our previous studies, Gengnianchun (GNC), a traditional Chinese medicine formula with a prolongevity effect, protected against Aβ-induced cytotoxicity in pheochromocytoma cells (PC-12 cells) and hippocampal cells. Here, we investigated the effects and possible mechanisms by which GNC protected against Aβ toxicity using transgenic Caenorhabditis elegans CL4176. Our results showed that GNC effectively delayed the Aβ toxicity-triggered body paralysis of CL4176 worms. GNC decreased Aβ by reducing Aβ mRNA levels. Moreover, GNC significantly reduced reactive oxygen species in the AD model worms compared with the controls. In addition, GNC upregulated the daf-16, sod-3, hsp-16.2 genes, and enhanced DAF-16 translocation from the cytoplasm to the nuclei under oxidative stress conditions. GNC treatment of C. elegans strains lacking DAF-16 did not affect the paralysis phenotype. Taken together, these findings suggest that GNC could protect against Aβ-induced toxicity via the DAF-16 pathway in C. elegans. Further studies are required to analyze its effectiveness in more complex animals.

Authors+Show Affiliations

Department of Gynecology, The First Hospital of Jilin University, Changchun, China. Department of Integrated Traditional Chinese Medicine and Western Medicine, Obstetrical and Gynecological Hospital, Fudan University, Shanghai, China.Department of Integrated Traditional Chinese Medicine and Western Medicine, Obstetrical and Gynecological Hospital, Fudan University, Shanghai, China.Department of Integrated Traditional Chinese Medicine and Western Medicine, Obstetrical and Gynecological Hospital, Fudan University, Shanghai, China.Department of Integrated Traditional Chinese Medicine and Western Medicine, Obstetrical and Gynecological Hospital, Fudan University, Shanghai, China.Department of Gynecology, The First Hospital of Jilin University, Changchun, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29234427

Citation

Meng, Fanhui, et al. "A Chinese Herbal Formula, Gengnianchun, Ameliorates β-Amyloid Peptide Toxicity in a Caenorhabditis Elegans Model of Alzheimer's Disease." Evidence-based Complementary and Alternative Medicine : ECAM, vol. 2017, 2017, p. 7480980.
Meng F, Li J, Rao Y, et al. A Chinese Herbal Formula, Gengnianchun, Ameliorates β-Amyloid Peptide Toxicity in a Caenorhabditis elegans Model of Alzheimer's Disease. Evid Based Complement Alternat Med. 2017;2017:7480980.
Meng, F., Li, J., Rao, Y., Wang, W., & Fu, Y. (2017). A Chinese Herbal Formula, Gengnianchun, Ameliorates β-Amyloid Peptide Toxicity in a Caenorhabditis elegans Model of Alzheimer's Disease. Evidence-based Complementary and Alternative Medicine : ECAM, 2017, 7480980. https://doi.org/10.1155/2017/7480980
Meng F, et al. A Chinese Herbal Formula, Gengnianchun, Ameliorates β-Amyloid Peptide Toxicity in a Caenorhabditis Elegans Model of Alzheimer's Disease. Evid Based Complement Alternat Med. 2017;2017:7480980. PubMed PMID: 29234427.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Chinese Herbal Formula, Gengnianchun, Ameliorates β-Amyloid Peptide Toxicity in a Caenorhabditis elegans Model of Alzheimer's Disease. AU - Meng,Fanhui, AU - Li,Jun, AU - Rao,Yanqiu, AU - Wang,Wenjun, AU - Fu,Yan, Y1 - 2017/10/11/ PY - 2017/04/26/received PY - 2017/08/26/revised PY - 2017/09/07/accepted PY - 2017/12/14/entrez PY - 2017/12/14/pubmed PY - 2017/12/14/medline SP - 7480980 EP - 7480980 JF - Evidence-based complementary and alternative medicine : eCAM JO - Evid Based Complement Alternat Med VL - 2017 N2 - Alzheimer's disease (AD) is an age-related neurodegenerative disorder, and the few drugs that are currently available only treat the symptoms. Traditional medicine or phytotherapy has been shown to protect against AD. In our previous studies, Gengnianchun (GNC), a traditional Chinese medicine formula with a prolongevity effect, protected against Aβ-induced cytotoxicity in pheochromocytoma cells (PC-12 cells) and hippocampal cells. Here, we investigated the effects and possible mechanisms by which GNC protected against Aβ toxicity using transgenic Caenorhabditis elegans CL4176. Our results showed that GNC effectively delayed the Aβ toxicity-triggered body paralysis of CL4176 worms. GNC decreased Aβ by reducing Aβ mRNA levels. Moreover, GNC significantly reduced reactive oxygen species in the AD model worms compared with the controls. In addition, GNC upregulated the daf-16, sod-3, hsp-16.2 genes, and enhanced DAF-16 translocation from the cytoplasm to the nuclei under oxidative stress conditions. GNC treatment of C. elegans strains lacking DAF-16 did not affect the paralysis phenotype. Taken together, these findings suggest that GNC could protect against Aβ-induced toxicity via the DAF-16 pathway in C. elegans. Further studies are required to analyze its effectiveness in more complex animals. SN - 1741-427X UR - https://www.unboundmedicine.com/medline/citation/29234427/A_Chinese_Herbal_Formula_Gengnianchun_Ameliorates_β_Amyloid_Peptide_Toxicity_in_a_Caenorhabditis_elegans_Model_of_Alzheimer's_Disease_ L2 - https://doi.org/10.1155/2017/7480980 DB - PRIME DP - Unbound Medicine ER -
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