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Determination of Acid and Neutral Cannabinoids in Extracts of Different Strains of Cannabis sativa Using GC-FID.
Planta Med 2018; 84(4):250-259PM

Abstract

Cannabis (Cannabis sativa L.) is an annual herbaceous plant that belongs to the family Cannabaceae. Trans-Δ9-tetrahydrocannabinol (Δ9-THC) and cannabidiol (CBD) are the two major phytocannabinoids accounting for over 40% of the cannabis plant extracts, depending on the variety. At the University of Mississippi, different strains of C. sativa, with different concentration ratios of CBD and Δ9-THC, have been tissue cultured via micropropagation and cultivated. A GC-FID method has been developed and validated for the qualitative and quantitative analysis of acid and neutral cannabinoids in C. sativa extracts. The method involves trimethyl silyl derivatization of the extracts. These cannabinoids include tetrahydrocannabivarian, CBD, cannabichromene, trans-Δ8-tetrahydrocannabinol, Δ9-THC, cannabigerol, cannabinol, cannabidiolic acid, cannabigerolic acid, and Δ9-tetrahydrocannabinolic acid-A. The concentration-response relationship of the method indicated a linear relationship between the concentration and peak area ratio with R2 > 0.999 for all 10 cannabinoids. The precision and accuracy of the method were found to be ≤ 15% and ± 5%, respectively. The limit of detection range was 0.11 - 0.19 µg/mL, and the limit of quantitation was 0.34 - 0.56 µg/mL for all 10 cannabinoids. The developed method is simple, sensitive, reproducible, and suitable for the detection and quantitation of acidic and neutral cannabinoids in different extracts of cannabis varieties. The method was applied to the analysis of these cannabinoids in different parts of the micropropagated cannabis plants (buds, leaves, roots, and stems).

Authors+Show Affiliations

National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, Mississippi, United States of America. Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, Mississippi, United States of America. ElSohly Laboratories Inc., 5 Industrial Park Drive, Oxford, Mississippi, United States of America.ElSohly Laboratories Inc., 5 Industrial Park Drive, Oxford, Mississippi, United States of America.ElSohly Laboratories Inc., 5 Industrial Park Drive, Oxford, Mississippi, United States of America. Department of Chemistry and Biochemistry, University of Mississippi, University, Mississippi, United States of America.Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, Mississippi, United States of America.National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, Mississippi, United States of America.National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, Mississippi, United States of America.National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, Mississippi, United States of America. ElSohly Laboratories Inc., 5 Industrial Park Drive, Oxford, Mississippi, United States of America. Department of Pharmaceutics and Drug Delivery, School of Pharmacy, The University of Mississippi, University, Mississippi, United States of America.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29237190

Citation

Ibrahim, Elsayed A., et al. "Determination of Acid and Neutral Cannabinoids in Extracts of Different Strains of Cannabis Sativa Using GC-FID." Planta Medica, vol. 84, no. 4, 2018, pp. 250-259.
Ibrahim EA, Gul W, Gul SW, et al. Determination of Acid and Neutral Cannabinoids in Extracts of Different Strains of Cannabis sativa Using GC-FID. Planta Med. 2018;84(4):250-259.
Ibrahim, E. A., Gul, W., Gul, S. W., Stamper, B. J., Hadad, G. M., Abdel Salam, R. A., ... ElSohly, M. A. (2018). Determination of Acid and Neutral Cannabinoids in Extracts of Different Strains of Cannabis sativa Using GC-FID. Planta Medica, 84(4), pp. 250-259. doi:10.1055/s-0043-124088.
Ibrahim EA, et al. Determination of Acid and Neutral Cannabinoids in Extracts of Different Strains of Cannabis Sativa Using GC-FID. Planta Med. 2018;84(4):250-259. PubMed PMID: 29237190.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of Acid and Neutral Cannabinoids in Extracts of Different Strains of Cannabis sativa Using GC-FID. AU - Ibrahim,Elsayed A, AU - Gul,Waseem, AU - Gul,Shahbaz W, AU - Stamper,Brandon J, AU - Hadad,Ghada M, AU - Abdel Salam,Randa A, AU - Ibrahim,Amany K, AU - Ahmed,Safwat A, AU - Chandra,Suman, AU - Lata,Hemant, AU - Radwan,Mohamed M, AU - ElSohly,Mahmoud A, Y1 - 2017/12/13/ PY - 2017/12/14/pubmed PY - 2018/8/10/medline PY - 2017/12/14/entrez SP - 250 EP - 259 JF - Planta medica JO - Planta Med. VL - 84 IS - 4 N2 - Cannabis (Cannabis sativa L.) is an annual herbaceous plant that belongs to the family Cannabaceae. Trans-Δ9-tetrahydrocannabinol (Δ9-THC) and cannabidiol (CBD) are the two major phytocannabinoids accounting for over 40% of the cannabis plant extracts, depending on the variety. At the University of Mississippi, different strains of C. sativa, with different concentration ratios of CBD and Δ9-THC, have been tissue cultured via micropropagation and cultivated. A GC-FID method has been developed and validated for the qualitative and quantitative analysis of acid and neutral cannabinoids in C. sativa extracts. The method involves trimethyl silyl derivatization of the extracts. These cannabinoids include tetrahydrocannabivarian, CBD, cannabichromene, trans-Δ8-tetrahydrocannabinol, Δ9-THC, cannabigerol, cannabinol, cannabidiolic acid, cannabigerolic acid, and Δ9-tetrahydrocannabinolic acid-A. The concentration-response relationship of the method indicated a linear relationship between the concentration and peak area ratio with R2 > 0.999 for all 10 cannabinoids. The precision and accuracy of the method were found to be ≤ 15% and ± 5%, respectively. The limit of detection range was 0.11 - 0.19 µg/mL, and the limit of quantitation was 0.34 - 0.56 µg/mL for all 10 cannabinoids. The developed method is simple, sensitive, reproducible, and suitable for the detection and quantitation of acidic and neutral cannabinoids in different extracts of cannabis varieties. The method was applied to the analysis of these cannabinoids in different parts of the micropropagated cannabis plants (buds, leaves, roots, and stems). SN - 1439-0221 UR - https://www.unboundmedicine.com/medline/citation/29237190/Determination_of_Acid_and_Neutral_Cannabinoids_in_Extracts_of_Different_Strains_of_Cannabis_sativa_Using_GC_FID_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-0043-124088 DB - PRIME DP - Unbound Medicine ER -