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Correlated production and consumption of chloromethane in the Arabidopsis thaliana phyllosphere.
Sci Rep 2017; 7(1):17589SR

Abstract

Chloromethane (CH3Cl) is a toxic gas mainly produced naturally, in particular by plants, and its emissions contribute to ozone destruction in the stratosphere. Conversely, CH3Cl can be degraded and used as the sole carbon and energy source by specialised methylotrophic bacteria, isolated from a variety of environments including the phyllosphere, i.e. the aerial parts of vegetation. The potential role of phyllospheric CH3Cl-degrading bacteria as a filter for plant emissions of CH3Cl was investigated using variants of Arabidopsis thaliana with low, wild-type and high expression of HOL1 methyltransferase previously shown to be responsible for most of CH3Cl emissions by A. thaliana. Presence and expression of the bacterial chloromethane dehalogenase cmuA gene in the A. thaliana phyllosphere correlated with HOL1 genotype, as shown by qPCR and RT-qPCR. Production of CH3Cl by A. thaliana paralleled HOL1 expression, as assessed by a fluorescence-based bioreporter. The relation between plant production of CH3Cl and relative abundance of CH3Cl-degrading bacteria in the phyllosphere suggests that CH3Cl-degrading bacteria co-determine the extent of plant emissions of CH3Cl to the atmosphere.

Authors+Show Affiliations

Université de Strasbourg, CNRS, GMGM UMR 7156, Department of Microbiology, Genomics and the Environment, Strasbourg, France. School of Environmental Sciences, University of East Anglia, Norwich, UK.Université de Strasbourg, CNRS, GMGM UMR 7156, Department of Microbiology, Genomics and the Environment, Strasbourg, France. Université de Reims Champagne Ardenne, INRA, FARE UMR, A614, Reims, France.Université de Strasbourg, CNRS, GMGM UMR 7156, Department of Microbiology, Genomics and the Environment, Strasbourg, France.Université de Strasbourg, CNRS, GMGM UMR 7156, Department of Microbiology, Genomics and the Environment, Strasbourg, France.Institut de Biologie Moléculaire des Plantes, UPR 2357 CNRS, Strasbourg, France.Institut de Biologie Moléculaire des Plantes, UPR 2357 CNRS, Strasbourg, France.Université de Strasbourg, CNRS, GMGM UMR 7156, Department of Microbiology, Genomics and the Environment, Strasbourg, France. vuilleumier@unistra.fr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29242530

Citation

Farhan Ul Haque, Muhammad, et al. "Correlated Production and Consumption of Chloromethane in the Arabidopsis Thaliana Phyllosphere." Scientific Reports, vol. 7, no. 1, 2017, p. 17589.
Farhan Ul Haque M, Besaury L, Nadalig T, et al. Correlated production and consumption of chloromethane in the Arabidopsis thaliana phyllosphere. Sci Rep. 2017;7(1):17589.
Farhan Ul Haque, M., Besaury, L., Nadalig, T., Bringel, F., Mutterer, J., Schaller, H., & Vuilleumier, S. (2017). Correlated production and consumption of chloromethane in the Arabidopsis thaliana phyllosphere. Scientific Reports, 7(1), p. 17589. doi:10.1038/s41598-017-17421-y.
Farhan Ul Haque M, et al. Correlated Production and Consumption of Chloromethane in the Arabidopsis Thaliana Phyllosphere. Sci Rep. 2017 12 14;7(1):17589. PubMed PMID: 29242530.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Correlated production and consumption of chloromethane in the Arabidopsis thaliana phyllosphere. AU - Farhan Ul Haque,Muhammad, AU - Besaury,Ludovic, AU - Nadalig,Thierry, AU - Bringel,Françoise, AU - Mutterer,Jérôme, AU - Schaller,Hubert, AU - Vuilleumier,Stéphane, Y1 - 2017/12/14/ PY - 2017/08/24/received PY - 2017/11/24/accepted PY - 2017/12/16/entrez PY - 2017/12/16/pubmed PY - 2019/7/12/medline SP - 17589 EP - 17589 JF - Scientific reports JO - Sci Rep VL - 7 IS - 1 N2 - Chloromethane (CH3Cl) is a toxic gas mainly produced naturally, in particular by plants, and its emissions contribute to ozone destruction in the stratosphere. Conversely, CH3Cl can be degraded and used as the sole carbon and energy source by specialised methylotrophic bacteria, isolated from a variety of environments including the phyllosphere, i.e. the aerial parts of vegetation. The potential role of phyllospheric CH3Cl-degrading bacteria as a filter for plant emissions of CH3Cl was investigated using variants of Arabidopsis thaliana with low, wild-type and high expression of HOL1 methyltransferase previously shown to be responsible for most of CH3Cl emissions by A. thaliana. Presence and expression of the bacterial chloromethane dehalogenase cmuA gene in the A. thaliana phyllosphere correlated with HOL1 genotype, as shown by qPCR and RT-qPCR. Production of CH3Cl by A. thaliana paralleled HOL1 expression, as assessed by a fluorescence-based bioreporter. The relation between plant production of CH3Cl and relative abundance of CH3Cl-degrading bacteria in the phyllosphere suggests that CH3Cl-degrading bacteria co-determine the extent of plant emissions of CH3Cl to the atmosphere. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/29242530/Correlated_production_and_consumption_of_chloromethane_in_the_Arabidopsis_thaliana_phyllosphere_ L2 - http://dx.doi.org/10.1038/s41598-017-17421-y DB - PRIME DP - Unbound Medicine ER -