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Emergence and genomic analysis of MDR Laribacter hongkongensis strain HLGZ1 from Guangzhou, China.
J Antimicrob Chemother. 2018 03 01; 73(3):643-647.JA

Abstract

Background

Laribacter hongkongensis is a facultative anaerobic, non-fermentative, Gram-negative bacillus associated with community-acquired gastroenteritis and traveller's diarrhoea. No clinical MDR L. hongkongensis isolate has been reported yet.

Methods

We performed WGS (PacBio and Illumina) on a clinical L. hongkongensis strain HLGZ1 with an MDR phenotype.

Results

HLGZ1 was resistant to eight classes of commonly used antibiotics. Its complete genome was a single circular chromosome of 3 424 272 bp with a G + C content of 62.29%. In comparison with the reference strain HLHK9, HLGZ1 had a higher abundance of genes associated with DNA metabolism and recombination. Several inserts including two acquired resistance gene clusters (RC1 and RC2) were also identified. RC1 carried two resistance gene cassette arrays, aac(6')-Ib-cr-aadA2-Δqac-Δsul1-floR-tetR-tetG and arr-3-dfrA32-ereA2-Δqac-sul1, which shared significant nucleotide sequence identities with the MDR region of Salmonella Genomic Island 1 from Salmonella enterica serovar Typhimurium DT104. There was also an integron-like structure, intl1-arr3-dfrA27-Δqac-sul1-aph(3')-Ic, and a tetR-tetA operon located on RC2. MLST analysis identified HLGZ1 as ST167, a novel ST clustered with two strains previously isolated from frogs.

Conclusions

This study provides insight into the genomic characteristics of MDR L. hongkongensis and highlights the possibilities of horizontal resistance gene transfer in this bacterium with other pathogens.

Authors+Show Affiliations

Department of Laboratory Medicine, State Key Laboratory of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510120, China.Laboratory of Genomic and Precision Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu 214122, China.Department of Laboratory Medicine, State Key Laboratory of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510120, China.Department of Laboratory Medicine, State Key Laboratory of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510120, China.Department of Laboratory Medicine, State Key Laboratory of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510120, China.Department of Laboratory Medicine, State Key Laboratory of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510120, China. Research Centre for Translational Medicine, First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510120, China.Department of Laboratory Medicine, State Key Laboratory of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510120, China. Research Centre for Translational Medicine, First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510120, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29244123

Citation

Wu, Hong-Kai, et al. "Emergence and Genomic Analysis of MDR Laribacter Hongkongensis Strain HLGZ1 From Guangzhou, China." The Journal of Antimicrobial Chemotherapy, vol. 73, no. 3, 2018, pp. 643-647.
Wu HK, Chen JH, Yang L, et al. Emergence and genomic analysis of MDR Laribacter hongkongensis strain HLGZ1 from Guangzhou, China. J Antimicrob Chemother. 2018;73(3):643-647.
Wu, H. K., Chen, J. H., Yang, L., Li, A. R., Su, D. H., Lin, Y. P., & Chen, D. Q. (2018). Emergence and genomic analysis of MDR Laribacter hongkongensis strain HLGZ1 from Guangzhou, China. The Journal of Antimicrobial Chemotherapy, 73(3), 643-647. https://doi.org/10.1093/jac/dkx470
Wu HK, et al. Emergence and Genomic Analysis of MDR Laribacter Hongkongensis Strain HLGZ1 From Guangzhou, China. J Antimicrob Chemother. 2018 03 1;73(3):643-647. PubMed PMID: 29244123.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Emergence and genomic analysis of MDR Laribacter hongkongensis strain HLGZ1 from Guangzhou, China. AU - Wu,Hong-Kai, AU - Chen,Jian-Huan, AU - Yang,Ling, AU - Li,A-Rong, AU - Su,Dan-Hong, AU - Lin,Yong-Ping, AU - Chen,Ding-Qiang, PY - 2017/08/04/received PY - 2017/11/13/accepted PY - 2017/12/16/pubmed PY - 2019/4/24/medline PY - 2017/12/16/entrez SP - 643 EP - 647 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 73 IS - 3 N2 - Background: Laribacter hongkongensis is a facultative anaerobic, non-fermentative, Gram-negative bacillus associated with community-acquired gastroenteritis and traveller's diarrhoea. No clinical MDR L. hongkongensis isolate has been reported yet. Methods: We performed WGS (PacBio and Illumina) on a clinical L. hongkongensis strain HLGZ1 with an MDR phenotype. Results: HLGZ1 was resistant to eight classes of commonly used antibiotics. Its complete genome was a single circular chromosome of 3 424 272 bp with a G + C content of 62.29%. In comparison with the reference strain HLHK9, HLGZ1 had a higher abundance of genes associated with DNA metabolism and recombination. Several inserts including two acquired resistance gene clusters (RC1 and RC2) were also identified. RC1 carried two resistance gene cassette arrays, aac(6')-Ib-cr-aadA2-Δqac-Δsul1-floR-tetR-tetG and arr-3-dfrA32-ereA2-Δqac-sul1, which shared significant nucleotide sequence identities with the MDR region of Salmonella Genomic Island 1 from Salmonella enterica serovar Typhimurium DT104. There was also an integron-like structure, intl1-arr3-dfrA27-Δqac-sul1-aph(3')-Ic, and a tetR-tetA operon located on RC2. MLST analysis identified HLGZ1 as ST167, a novel ST clustered with two strains previously isolated from frogs. Conclusions: This study provides insight into the genomic characteristics of MDR L. hongkongensis and highlights the possibilities of horizontal resistance gene transfer in this bacterium with other pathogens. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/29244123/Emergence_and_genomic_analysis_of_MDR_Laribacter_hongkongensis_strain_HLGZ1_from_Guangzhou_China_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkx470 DB - PRIME DP - Unbound Medicine ER -