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Poor performance of two rapid immunochromatographic assays for anti-Japanese encephalitis virus immunoglobulin M detection in cerebrospinal fluid and serum from patients with suspected Japanese encephalitis virus infection in Laos.
Trans R Soc Trop Med Hyg. 2017 08 01; 111(8):373-377.TR

Abstract

Background

Japanese encephalitis virus (JEV) is a leading identified cause of encephalitis in Asia, often occurring in rural areas with poor access to laboratory diagnostics. We evaluated two rapid diagnostic tests (RDTs) for anti-JEV immunoglobulin M (IgM) detection.

Methods

Consecutive cerebrospinal fluid and serum from 388 patients (704 samples) with suspected JEV infections admitted to six hospitals in Laos were tested with one of two SD-Bioline anti-JEV IgM RDTs and the World Health Organization standard anti-JEV IgM enzyme-linked immunosorbent assay (ELISA; Panbio Japanese Encephalitis-Dengue IgM Combo ELISA.

Results and Conclusions

The performance of both RDTs showed strikingly low sensitivity in comparison to anti-JEV IgM antibody capture ELISA (2.1-51.4%), suggesting low sensitivity of the RDTs. We highlight the fundamental prerequisite to validate RDTs prior to use to ensure that they meet standards for testing.

Authors+Show Affiliations

Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic.Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic. Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Churchill Hospital, Oxford, UK.Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic.Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic.Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic.Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic.Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic. Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Churchill Hospital, Oxford, UK.Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic. Division of Infection and Immunity, University College London, London, UK.Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic. Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Churchill Hospital, Oxford, UK. UMR 'Émergence des Pathologies Virales' (EPV), Aix-Marseille Université, IRD 190, Inserm 1207, EHESP, IHU Méditerranée Infection, Marseille, France.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29244182

Citation

Sengvilaipaseuth, Onanong, et al. "Poor Performance of Two Rapid Immunochromatographic Assays for anti-Japanese Encephalitis Virus Immunoglobulin M Detection in Cerebrospinal Fluid and Serum From Patients With Suspected Japanese Encephalitis Virus Infection in Laos." Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 111, no. 8, 2017, pp. 373-377.
Sengvilaipaseuth O, Castonguay-Vanier J, Chanthongthip A, et al. Poor performance of two rapid immunochromatographic assays for anti-Japanese encephalitis virus immunoglobulin M detection in cerebrospinal fluid and serum from patients with suspected Japanese encephalitis virus infection in Laos. Trans R Soc Trop Med Hyg. 2017;111(8):373-377.
Sengvilaipaseuth, O., Castonguay-Vanier, J., Chanthongthip, A., Phonemixay, O., Thongpaseuth, S., Vongsouvath, M., Newton, P. N., Bharucha, T., & Dubot-Pérès, A. (2017). Poor performance of two rapid immunochromatographic assays for anti-Japanese encephalitis virus immunoglobulin M detection in cerebrospinal fluid and serum from patients with suspected Japanese encephalitis virus infection in Laos. Transactions of the Royal Society of Tropical Medicine and Hygiene, 111(8), 373-377. https://doi.org/10.1093/trstmh/trx067
Sengvilaipaseuth O, et al. Poor Performance of Two Rapid Immunochromatographic Assays for anti-Japanese Encephalitis Virus Immunoglobulin M Detection in Cerebrospinal Fluid and Serum From Patients With Suspected Japanese Encephalitis Virus Infection in Laos. Trans R Soc Trop Med Hyg. 2017 08 1;111(8):373-377. PubMed PMID: 29244182.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Poor performance of two rapid immunochromatographic assays for anti-Japanese encephalitis virus immunoglobulin M detection in cerebrospinal fluid and serum from patients with suspected Japanese encephalitis virus infection in Laos. AU - Sengvilaipaseuth,Onanong, AU - Castonguay-Vanier,Josée, AU - Chanthongthip,Anisone, AU - Phonemixay,Ooyanong, AU - Thongpaseuth,Soulignasack, AU - Vongsouvath,Manivanh, AU - Newton,Paul N, AU - Bharucha,Tehmina, AU - Dubot-Pérès,Audrey, PY - 2017/07/09/received PY - 2017/10/24/accepted PY - 2017/12/16/pubmed PY - 2018/12/12/medline PY - 2017/12/16/entrez KW - Central nervous system infection KW - Immunoassays KW - Japanese encephalitis virus KW - Laos, Rapid diagnostic tests KW - South-East Asia SP - 373 EP - 377 JF - Transactions of the Royal Society of Tropical Medicine and Hygiene JO - Trans R Soc Trop Med Hyg VL - 111 IS - 8 N2 - Background: Japanese encephalitis virus (JEV) is a leading identified cause of encephalitis in Asia, often occurring in rural areas with poor access to laboratory diagnostics. We evaluated two rapid diagnostic tests (RDTs) for anti-JEV immunoglobulin M (IgM) detection. Methods: Consecutive cerebrospinal fluid and serum from 388 patients (704 samples) with suspected JEV infections admitted to six hospitals in Laos were tested with one of two SD-Bioline anti-JEV IgM RDTs and the World Health Organization standard anti-JEV IgM enzyme-linked immunosorbent assay (ELISA; Panbio Japanese Encephalitis-Dengue IgM Combo ELISA. Results and Conclusions: The performance of both RDTs showed strikingly low sensitivity in comparison to anti-JEV IgM antibody capture ELISA (2.1-51.4%), suggesting low sensitivity of the RDTs. We highlight the fundamental prerequisite to validate RDTs prior to use to ensure that they meet standards for testing. SN - 1878-3503 UR - https://www.unboundmedicine.com/medline/citation/29244182/Poor_performance_of_two_rapid_immunochromatographic_assays_for_anti_Japanese_encephalitis_virus_immunoglobulin_M_detection_in_cerebrospinal_fluid_and_serum_from_patients_with_suspected_Japanese_encephalitis_virus_infection_in_Laos_ L2 - https://academic.oup.com/trstmh/article-lookup/doi/10.1093/trstmh/trx067 DB - PRIME DP - Unbound Medicine ER -