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Oocyte oxidative DNA damage may be involved in minimal/mild endometriosis-related infertility.
Mol Reprod Dev. 2018 02; 85(2):128-136.MR

Abstract

Early endometriosis is associated with infertility, and oxidative stress may play a role in the pathogenesis of disease-related infertility. This prospective case-control study aimed to compare the presence of oxidative stress markers in the follicular microenvironment and systemic circulation of infertile women with minimal/mild endometriosis (EI/II) versus individuals undergoing controlled ovarian stimulation for intracytoplasmic sperm injection (ICSI). Seventy-one blood samples (27 from infertile women with EI/II and 44 controls with tubal and/or male infertility factor) and 51 follicular fluid samples (19 EI/II and 32 controls) were obtained on the day of oocyte retrieval. Total hydroperoxides (FOX1), reduced glutathione, vitamin E, Superoxide dismutase, total antioxidant capacity, malondialdehyde, advanced oxidation protein products, and 8-hydroxy-2'-deoxyguanosine (8OHdG) concentrations were measured in both fluids. Women with EI/II showed higher FOX1 (8.48 ± 1.72 vs. 7.69 ± 1.71 μmol/g protein) and lower total antioxidant capacity (0.38 ± 0.18 vs. 0.46 ± 0.15 mEq Trolox/L) concentrations in serum, and higher 8OHdG concentrations (24.21 ± 8.56 vs. 17.22 ± 5.6 ng/ml) in follicular fluid compared with controls. These data implicate both systemic and follicular oxidative stress may in infertile women with EI/II undergoing controlled ovarian stimulation for ICSI. Furthermore, the elevated 8OHdG concentrations in follicular fluid of women with EI/II may be related to compromised oocyte quality.

Authors+Show Affiliations

Human Reproduction Division, Department of Obstetrics and Gynecology, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.Nutrition and Metabolism Laboratory, Ribeirão Preto School of Medicine, University of Sao Paulo, Ribeirão Preto, São Paulo, Brazil.Human Reproduction Division, Department of Obstetrics and Gynecology, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. National Institute of Hormones and Woman's Health, CNPq, Porto Alegre, Brazil.Human Reproduction Division, Department of Obstetrics and Gynecology, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. National Institute of Hormones and Woman's Health, CNPq, Porto Alegre, Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29247565

Citation

Da Broi, Michele G., et al. "Oocyte Oxidative DNA Damage May Be Involved in Minimal/mild Endometriosis-related Infertility." Molecular Reproduction and Development, vol. 85, no. 2, 2018, pp. 128-136.
Da Broi MG, Jordão AA, Ferriani RA, et al. Oocyte oxidative DNA damage may be involved in minimal/mild endometriosis-related infertility. Mol Reprod Dev. 2018;85(2):128-136.
Da Broi, M. G., Jordão, A. A., Ferriani, R. A., & Navarro, P. A. (2018). Oocyte oxidative DNA damage may be involved in minimal/mild endometriosis-related infertility. Molecular Reproduction and Development, 85(2), 128-136. https://doi.org/10.1002/mrd.22943
Da Broi MG, et al. Oocyte Oxidative DNA Damage May Be Involved in Minimal/mild Endometriosis-related Infertility. Mol Reprod Dev. 2018;85(2):128-136. PubMed PMID: 29247565.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oocyte oxidative DNA damage may be involved in minimal/mild endometriosis-related infertility. AU - Da Broi,Michele G, AU - Jordão,Alceu A,Jr AU - Ferriani,Rui A, AU - Navarro,Paula A, Y1 - 2018/01/17/ PY - 2017/10/05/received PY - 2017/12/05/revised PY - 2017/12/11/accepted PY - 2017/12/17/pubmed PY - 2019/7/4/medline PY - 2017/12/17/entrez KW - 8-hydroxy-2′-deoxyguanosine KW - early endometriosis KW - follicular fluid KW - oxidative stress SP - 128 EP - 136 JF - Molecular reproduction and development JO - Mol. Reprod. Dev. VL - 85 IS - 2 N2 - Early endometriosis is associated with infertility, and oxidative stress may play a role in the pathogenesis of disease-related infertility. This prospective case-control study aimed to compare the presence of oxidative stress markers in the follicular microenvironment and systemic circulation of infertile women with minimal/mild endometriosis (EI/II) versus individuals undergoing controlled ovarian stimulation for intracytoplasmic sperm injection (ICSI). Seventy-one blood samples (27 from infertile women with EI/II and 44 controls with tubal and/or male infertility factor) and 51 follicular fluid samples (19 EI/II and 32 controls) were obtained on the day of oocyte retrieval. Total hydroperoxides (FOX1), reduced glutathione, vitamin E, Superoxide dismutase, total antioxidant capacity, malondialdehyde, advanced oxidation protein products, and 8-hydroxy-2'-deoxyguanosine (8OHdG) concentrations were measured in both fluids. Women with EI/II showed higher FOX1 (8.48 ± 1.72 vs. 7.69 ± 1.71 μmol/g protein) and lower total antioxidant capacity (0.38 ± 0.18 vs. 0.46 ± 0.15 mEq Trolox/L) concentrations in serum, and higher 8OHdG concentrations (24.21 ± 8.56 vs. 17.22 ± 5.6 ng/ml) in follicular fluid compared with controls. These data implicate both systemic and follicular oxidative stress may in infertile women with EI/II undergoing controlled ovarian stimulation for ICSI. Furthermore, the elevated 8OHdG concentrations in follicular fluid of women with EI/II may be related to compromised oocyte quality. SN - 1098-2795 UR - https://www.unboundmedicine.com/medline/citation/29247565/Oocyte_oxidative_DNA_damage_may_be_involved_in_minimal/mild_endometriosis_related_infertility_ L2 - https://doi.org/10.1002/mrd.22943 DB - PRIME DP - Unbound Medicine ER -