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Breastfeeding-associated microbiota in human milk following supplementation with Lactobacillus rhamnosus GG, Lactobacillus acidophilus La-5, and Bifidobacterium animalis ssp. lactis Bb-12.
J Dairy Sci. 2018 Feb; 101(2):889-899.JD

Abstract

Breastfeeding is one of the major factors affecting the early development of the infant gut microbiota, and weaning is associated with a shift in the gut microbiota toward a more adult composition. Through breastfeeding, infants receive bioactive components that shape their microbiota while also being exposed to the breast milk and breast surface microbial communities. Recent studies have suggested the possibility of an entero-mammary route of microbial transfer, opening the possibility of infant gut microbiota modulation through maternal probiotic supplementation. In this study, we have analyzed breast milk samples collected at 10 d and 3 mo postpartum from women participating in the Probiotics in the Prevention of Allergy among Children in Trondheim placebo controlled trial. Women who were randomized to the probiotic arm of the Probiotics in the Prevention of Allergy among Children in Trondheim trial received a fermented milk supplemented with Lactobacillus rhamnosus GG, Lactobacillus acidophilus La-5, and Bifidobacterium animalis ssp. lactis Bb-12, consuming this daily from 4 wk before their expected due date until 3 mo after birth. In total, 472 breast milk samples were assessed for the administered bacteria using quantitative real-time PCR and the microbiota transferred during breastfeeding was analyzed using 16S ribosomal RNA gene sequencing of 142 samples. We found that breastfeeding is unlikely to be a significant source of L. rhamnosus GG, L. acidophilus La-5, and B. animalis ssp. lactis Bb-12 for infants in the probiotic arm of the trial. Furthermore, maternal supplementation did not significantly affect the overall composition of the breast milk microbiota transferred during breastfeeding. We also present a descriptive analysis of this microbiota, which was largely dominated by Streptococcus and Staphylococcus genera at both 10 d and 3 mo postpartum. Samples collected at 3 mo postpartum had a statistically significant lower presence and relative abundance of the Staphylococcus genus. These samples also had a greater number of observed species and diversity, including more operational taxonomic units from the Rothia, Veillonella, Granulicatella, and Methylbacterium genera.

Authors+Show Affiliations

Department of Public Health and Nursing, NTNU - Norwegian University of Science and Technology, N-7030 Trondheim, Norway. Electronic address: melanie.simpson@ntnu.no.Department of Chemistry, Biotechnology and Food Science, University of Life Sciences, N-1432 Ås, Norway.Department of Public Health and Nursing, NTNU - Norwegian University of Science and Technology, N-7030 Trondheim, Norway.Department of Public Health and Nursing, NTNU - Norwegian University of Science and Technology, N-7030 Trondheim, Norway.Department of Chemistry, Biotechnology and Food Science, University of Life Sciences, N-1432 Ås, Norway.Department of Public Health and Nursing, NTNU - Norwegian University of Science and Technology, N-7030 Trondheim, Norway.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29248229

Citation

Simpson, Melanie Rae, et al. "Breastfeeding-associated Microbiota in Human Milk Following Supplementation With Lactobacillus Rhamnosus GG, Lactobacillus Acidophilus La-5, and Bifidobacterium Animalis Ssp. Lactis Bb-12." Journal of Dairy Science, vol. 101, no. 2, 2018, pp. 889-899.
Simpson MR, Avershina E, Storrø O, et al. Breastfeeding-associated microbiota in human milk following supplementation with Lactobacillus rhamnosus GG, Lactobacillus acidophilus La-5, and Bifidobacterium animalis ssp. lactis Bb-12. J Dairy Sci. 2018;101(2):889-899.
Simpson, M. R., Avershina, E., Storrø, O., Johnsen, R., Rudi, K., & Øien, T. (2018). Breastfeeding-associated microbiota in human milk following supplementation with Lactobacillus rhamnosus GG, Lactobacillus acidophilus La-5, and Bifidobacterium animalis ssp. lactis Bb-12. Journal of Dairy Science, 101(2), 889-899. https://doi.org/10.3168/jds.2017-13411
Simpson MR, et al. Breastfeeding-associated Microbiota in Human Milk Following Supplementation With Lactobacillus Rhamnosus GG, Lactobacillus Acidophilus La-5, and Bifidobacterium Animalis Ssp. Lactis Bb-12. J Dairy Sci. 2018;101(2):889-899. PubMed PMID: 29248229.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Breastfeeding-associated microbiota in human milk following supplementation with Lactobacillus rhamnosus GG, Lactobacillus acidophilus La-5, and Bifidobacterium animalis ssp. lactis Bb-12. AU - Simpson,Melanie Rae, AU - Avershina,Ekaterina, AU - Storrø,Ola, AU - Johnsen,Roar, AU - Rudi,Knut, AU - Øien,Torbjørn, Y1 - 2017/12/14/ PY - 2017/06/29/received PY - 2017/10/17/accepted PY - 2017/12/19/pubmed PY - 2018/8/4/medline PY - 2017/12/18/entrez KW - atopic dermatitis KW - human milk KW - microbiota KW - probiotics SP - 889 EP - 899 JF - Journal of dairy science JO - J. Dairy Sci. VL - 101 IS - 2 N2 - Breastfeeding is one of the major factors affecting the early development of the infant gut microbiota, and weaning is associated with a shift in the gut microbiota toward a more adult composition. Through breastfeeding, infants receive bioactive components that shape their microbiota while also being exposed to the breast milk and breast surface microbial communities. Recent studies have suggested the possibility of an entero-mammary route of microbial transfer, opening the possibility of infant gut microbiota modulation through maternal probiotic supplementation. In this study, we have analyzed breast milk samples collected at 10 d and 3 mo postpartum from women participating in the Probiotics in the Prevention of Allergy among Children in Trondheim placebo controlled trial. Women who were randomized to the probiotic arm of the Probiotics in the Prevention of Allergy among Children in Trondheim trial received a fermented milk supplemented with Lactobacillus rhamnosus GG, Lactobacillus acidophilus La-5, and Bifidobacterium animalis ssp. lactis Bb-12, consuming this daily from 4 wk before their expected due date until 3 mo after birth. In total, 472 breast milk samples were assessed for the administered bacteria using quantitative real-time PCR and the microbiota transferred during breastfeeding was analyzed using 16S ribosomal RNA gene sequencing of 142 samples. We found that breastfeeding is unlikely to be a significant source of L. rhamnosus GG, L. acidophilus La-5, and B. animalis ssp. lactis Bb-12 for infants in the probiotic arm of the trial. Furthermore, maternal supplementation did not significantly affect the overall composition of the breast milk microbiota transferred during breastfeeding. We also present a descriptive analysis of this microbiota, which was largely dominated by Streptococcus and Staphylococcus genera at both 10 d and 3 mo postpartum. Samples collected at 3 mo postpartum had a statistically significant lower presence and relative abundance of the Staphylococcus genus. These samples also had a greater number of observed species and diversity, including more operational taxonomic units from the Rothia, Veillonella, Granulicatella, and Methylbacterium genera. SN - 1525-3198 UR - https://www.unboundmedicine.com/medline/citation/29248229/Breastfeeding_associated_microbiota_in_human_milk_following_supplementation_with_Lactobacillus_rhamnosus_GG_Lactobacillus_acidophilus_La_5_and_Bifidobacterium_animalis_ssp__lactis_Bb_12_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-0302(17)31152-9 DB - PRIME DP - Unbound Medicine ER -