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Chromatographic Methods for Quantitative Determination of Ampicillin, Dicloxacillin and Their Impurity 6-Aminopenicillanic Acid.
J Chromatogr Sci 2018; 56(3):209-215JC

Abstract

Two accurate, precise and sensitive high-performance thin layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC) methods were developed for assay of ampicillin (AMP) and dicloxacillin (DX) in the presence of their impurity, 6-aminopenicillanic acid (APA). Method (A) is HPTLC method; using silica gel HPTLC F254 plates as a stationary phase with methanol: chloroform: acetic acid (1:9: 0.2, by volume) as a developing system. All the bands were scanned at 220 nm. Method (B) is reversed phase- HPLC which depended on isocratic elution using C18 column and mobile phase consisting of acetonitrile: water (60:40, v/v), pH adjusted to 4 with orthophosphoric acid, at a flow rate of 1 mL min-1 and ultraviolet detection at 240 nm. The proposed methods were validated as per ICH guidelines and their linearity was evident in the ranges of 0.5-2 μg band-1, 0.4-2 μg band-1 and 0.2-1.2 μg band-1 for method (A) and 5-40 μg mL-1, 5-40 μg mL-1 and 2-16 μg mL-1 for method (B) for AMP, DX and APA, respectively. The proposed methods were successfully used for assay of AMP and DX in pure form and in pharmaceutical formulation where no interference from the excipients was detected.

Authors+Show Affiliations

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, AlshaheedShehata Ahmad Hegazy St., Beni-Suef 62514, Egypt.Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, AlshaheedShehata Ahmad Hegazy St., Beni-Suef 62514, Egypt.Analytical Chemistry Department, Faculty of Pharmacy, Fayoum University, Fayoum 63514, Egypt.Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St., Cairo 11562, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29253094

Citation

Abdelrahman, Maha M., et al. "Chromatographic Methods for Quantitative Determination of Ampicillin, Dicloxacillin and Their Impurity 6-Aminopenicillanic Acid." Journal of Chromatographic Science, vol. 56, no. 3, 2018, pp. 209-215.
Abdelrahman MM, Naguib IA, Elsayed MA, et al. Chromatographic Methods for Quantitative Determination of Ampicillin, Dicloxacillin and Their Impurity 6-Aminopenicillanic Acid. J Chromatogr Sci. 2018;56(3):209-215.
Abdelrahman, M. M., Naguib, I. A., Elsayed, M. A., & Zaazaa, H. A. (2018). Chromatographic Methods for Quantitative Determination of Ampicillin, Dicloxacillin and Their Impurity 6-Aminopenicillanic Acid. Journal of Chromatographic Science, 56(3), pp. 209-215. doi:10.1093/chromsci/bmx101.
Abdelrahman MM, et al. Chromatographic Methods for Quantitative Determination of Ampicillin, Dicloxacillin and Their Impurity 6-Aminopenicillanic Acid. J Chromatogr Sci. 2018 Mar 1;56(3):209-215. PubMed PMID: 29253094.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chromatographic Methods for Quantitative Determination of Ampicillin, Dicloxacillin and Their Impurity 6-Aminopenicillanic Acid. AU - Abdelrahman,Maha M, AU - Naguib,Ibrahim A, AU - Elsayed,Mohamed A, AU - Zaazaa,Hala A, PY - 2016/02/08/received PY - 2017/12/19/pubmed PY - 2018/3/27/medline PY - 2017/12/19/entrez SP - 209 EP - 215 JF - Journal of chromatographic science JO - J Chromatogr Sci VL - 56 IS - 3 N2 - Two accurate, precise and sensitive high-performance thin layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC) methods were developed for assay of ampicillin (AMP) and dicloxacillin (DX) in the presence of their impurity, 6-aminopenicillanic acid (APA). Method (A) is HPTLC method; using silica gel HPTLC F254 plates as a stationary phase with methanol: chloroform: acetic acid (1:9: 0.2, by volume) as a developing system. All the bands were scanned at 220 nm. Method (B) is reversed phase- HPLC which depended on isocratic elution using C18 column and mobile phase consisting of acetonitrile: water (60:40, v/v), pH adjusted to 4 with orthophosphoric acid, at a flow rate of 1 mL min-1 and ultraviolet detection at 240 nm. The proposed methods were validated as per ICH guidelines and their linearity was evident in the ranges of 0.5-2 μg band-1, 0.4-2 μg band-1 and 0.2-1.2 μg band-1 for method (A) and 5-40 μg mL-1, 5-40 μg mL-1 and 2-16 μg mL-1 for method (B) for AMP, DX and APA, respectively. The proposed methods were successfully used for assay of AMP and DX in pure form and in pharmaceutical formulation where no interference from the excipients was detected. SN - 1945-239X UR - https://www.unboundmedicine.com/medline/citation/29253094/Chromatographic_Methods_for_Quantitative_Determination_of_Ampicillin_Dicloxacillin_and_Their_Impurity_6_Aminopenicillanic_Acid_ L2 - https://academic.oup.com/chromsci/article-lookup/doi/10.1093/chromsci/bmx101 DB - PRIME DP - Unbound Medicine ER -