Tags

Type your tag names separated by a space and hit enter

Science and Bioethics of CRISPR-Cas9 Gene Editing: An Analysis Towards Separating Facts and Fiction.
Yale J Biol Med. 2017 12; 90(4):625-634.YJ

Abstract

Since its emergence in 2012, the genome editing technique known as CRISPR-Cas9 and its scientific use have rapidly expanded globally within a very short period of time. The technique consists of using an RNA guide molecule to bind to complementary DNA sequences, which simultaneously recruits the endonuclease Cas9 to introduce double-stranded breaks in the target DNA. The resulting double-stranded break is then repaired, allowing modification or removal of specific DNA bases. The technique has gained momentum in the laboratory because it is cheap, quick, and easy to use. Moreover, it is also being applied in vivo to generate more complex animal model systems. Such use of genome editing has proven to be highly effective and warrants a potential therapy for both genetic and non-genetic diseases. Although genome editing has the potential to be a transformative therapy for patients it is still in its infancy. Consequently, the legal and ethical frameworks are yet to be fully discussed and will be an increasingly important topic as the technology moves towards more contentious issues such as modification of the germline. Here, we review a number of scientific and ethical issues which may potentially influence the development of both the technology and its use in the clinical setting.

Authors+Show Affiliations

Computational Genomics and Training Centre (CGAT), MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, Oxford, UK. Campion Hall, Oxford, UK.Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK. Campion Hall, Oxford, UK.

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

29259526

Citation

Cribbs, Adam P., and Sumeth M W. Perera. "Science and Bioethics of CRISPR-Cas9 Gene Editing: an Analysis Towards Separating Facts and Fiction." The Yale Journal of Biology and Medicine, vol. 90, no. 4, 2017, pp. 625-634.
Cribbs AP, Perera SMW. Science and Bioethics of CRISPR-Cas9 Gene Editing: An Analysis Towards Separating Facts and Fiction. Yale J Biol Med. 2017;90(4):625-634.
Cribbs, A. P., & Perera, S. M. W. (2017). Science and Bioethics of CRISPR-Cas9 Gene Editing: An Analysis Towards Separating Facts and Fiction. The Yale Journal of Biology and Medicine, 90(4), 625-634.
Cribbs AP, Perera SMW. Science and Bioethics of CRISPR-Cas9 Gene Editing: an Analysis Towards Separating Facts and Fiction. Yale J Biol Med. 2017;90(4):625-634. PubMed PMID: 29259526.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Science and Bioethics of CRISPR-Cas9 Gene Editing: An Analysis Towards Separating Facts and Fiction. AU - Cribbs,Adam P, AU - Perera,Sumeth M W, Y1 - 2017/12/19/ PY - 2017/12/21/entrez PY - 2017/12/21/pubmed PY - 2018/7/20/medline KW - CRISPR KW - Cas9 KW - bioethics KW - genome editing SP - 625 EP - 634 JF - The Yale journal of biology and medicine JO - Yale J Biol Med VL - 90 IS - 4 N2 - Since its emergence in 2012, the genome editing technique known as CRISPR-Cas9 and its scientific use have rapidly expanded globally within a very short period of time. The technique consists of using an RNA guide molecule to bind to complementary DNA sequences, which simultaneously recruits the endonuclease Cas9 to introduce double-stranded breaks in the target DNA. The resulting double-stranded break is then repaired, allowing modification or removal of specific DNA bases. The technique has gained momentum in the laboratory because it is cheap, quick, and easy to use. Moreover, it is also being applied in vivo to generate more complex animal model systems. Such use of genome editing has proven to be highly effective and warrants a potential therapy for both genetic and non-genetic diseases. Although genome editing has the potential to be a transformative therapy for patients it is still in its infancy. Consequently, the legal and ethical frameworks are yet to be fully discussed and will be an increasingly important topic as the technology moves towards more contentious issues such as modification of the germline. Here, we review a number of scientific and ethical issues which may potentially influence the development of both the technology and its use in the clinical setting. SN - 1551-4056 UR - https://www.unboundmedicine.com/medline/citation/29259526/Science_and_Bioethics_of_CRISPR_Cas9_Gene_Editing:_An_Analysis_Towards_Separating_Facts_and_Fiction_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/29259526/ DB - PRIME DP - Unbound Medicine ER -