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Gold nanoparticle core-europium(iii) chelate fluorophore-doped silica shell hybrid nanocomposites for the lateral flow immunoassay of human thyroid stimulating hormone with a dual signal readout.
Analyst 2018; 143(2):564-570A

Abstract

Hybrid nanocomposite particles composed of a gold core coated with a europium(iii)-chelate fluorophore-doped silica shell (AuNPs@SiO2-Eu3+) have been synthesized and applied as antibody labels in lateral flow immunoassay (LFIA) devices for the determination of human thyroid stimulating hormone (hTSH). Labeling of monoclonal anti-hTSH antibodies with AuNPs@SiO2-Eu3+ nanocomposites allows for both colorimetric and fluorometric observation of assay results on LFIA devices, relying on visible light absorption due to the localized surface plasmon resonance of the Au-core and the fluorescence emission of the Eu(iii)-chelate-modified shell under UV hand lamp irradiation (365 nm), respectively. The possibility for a dual signal readout provides an attractive alternative for LFIAs: instantaneous naked eye observation of the AuNP colorimetric signal as in conventional LFIAs for hypothyroidism detection, and more sensitive fluorescence detection to assess hyperthyroidism. The limits of detection (LOD) for naked eye observation of LFIA devices are 5 μIU mL-1 and 0.1 μIU mL-1 for the colorimetric and fluorimetric detection, respectively. Using the fluorescence detection scheme in combination with a smartphone and digital color analysis, a quantitative linear relationship between the red intensity and the logarithmic concentration of hTSH was observed (R2 = 0.988) with an LOD of 0.02 μIU mL-1. Finally, LFIA devices were effectively applied for detecting hTSH in spiked diluted human serum with recovery values between 100-116%.

Authors+Show Affiliations

Program in Biotechnology, Faculty of Science, Chulalongkorn University, Patumwan, Bangkok, 10330, Thailand.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29260807

Citation

Preechakasedkit, Pattarachaya, et al. "Gold Nanoparticle Core-europium(iii) Chelate Fluorophore-doped Silica Shell Hybrid Nanocomposites for the Lateral Flow Immunoassay of Human Thyroid Stimulating Hormone With a Dual Signal Readout." The Analyst, vol. 143, no. 2, 2018, pp. 564-570.
Preechakasedkit P, Osada K, Katayama Y, et al. Gold nanoparticle core-europium(iii) chelate fluorophore-doped silica shell hybrid nanocomposites for the lateral flow immunoassay of human thyroid stimulating hormone with a dual signal readout. Analyst. 2018;143(2):564-570.
Preechakasedkit, P., Osada, K., Katayama, Y., Ruecha, N., Suzuki, K., Chailapakul, O., & Citterio, D. (2018). Gold nanoparticle core-europium(iii) chelate fluorophore-doped silica shell hybrid nanocomposites for the lateral flow immunoassay of human thyroid stimulating hormone with a dual signal readout. The Analyst, 143(2), pp. 564-570. doi:10.1039/c7an01799e.
Preechakasedkit P, et al. Gold Nanoparticle Core-europium(iii) Chelate Fluorophore-doped Silica Shell Hybrid Nanocomposites for the Lateral Flow Immunoassay of Human Thyroid Stimulating Hormone With a Dual Signal Readout. Analyst. 2018 Jan 21;143(2):564-570. PubMed PMID: 29260807.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gold nanoparticle core-europium(iii) chelate fluorophore-doped silica shell hybrid nanocomposites for the lateral flow immunoassay of human thyroid stimulating hormone with a dual signal readout. AU - Preechakasedkit,Pattarachaya, AU - Osada,Kota, AU - Katayama,Yuta, AU - Ruecha,Nipapan, AU - Suzuki,Koji, AU - Chailapakul,Orawon, AU - Citterio,Daniel, Y1 - 2017/12/20/ PY - 2017/12/21/pubmed PY - 2018/11/7/medline PY - 2017/12/21/entrez SP - 564 EP - 570 JF - The Analyst JO - Analyst VL - 143 IS - 2 N2 - Hybrid nanocomposite particles composed of a gold core coated with a europium(iii)-chelate fluorophore-doped silica shell (AuNPs@SiO2-Eu3+) have been synthesized and applied as antibody labels in lateral flow immunoassay (LFIA) devices for the determination of human thyroid stimulating hormone (hTSH). Labeling of monoclonal anti-hTSH antibodies with AuNPs@SiO2-Eu3+ nanocomposites allows for both colorimetric and fluorometric observation of assay results on LFIA devices, relying on visible light absorption due to the localized surface plasmon resonance of the Au-core and the fluorescence emission of the Eu(iii)-chelate-modified shell under UV hand lamp irradiation (365 nm), respectively. The possibility for a dual signal readout provides an attractive alternative for LFIAs: instantaneous naked eye observation of the AuNP colorimetric signal as in conventional LFIAs for hypothyroidism detection, and more sensitive fluorescence detection to assess hyperthyroidism. The limits of detection (LOD) for naked eye observation of LFIA devices are 5 μIU mL-1 and 0.1 μIU mL-1 for the colorimetric and fluorimetric detection, respectively. Using the fluorescence detection scheme in combination with a smartphone and digital color analysis, a quantitative linear relationship between the red intensity and the logarithmic concentration of hTSH was observed (R2 = 0.988) with an LOD of 0.02 μIU mL-1. Finally, LFIA devices were effectively applied for detecting hTSH in spiked diluted human serum with recovery values between 100-116%. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/29260807/Gold_nanoparticle_core_europium_iii__chelate_fluorophore_doped_silica_shell_hybrid_nanocomposites_for_the_lateral_flow_immunoassay_of_human_thyroid_stimulating_hormone_with_a_dual_signal_readout_ L2 - https://doi.org/10.1039/c7an01799e DB - PRIME DP - Unbound Medicine ER -