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Molecular cloning and analysis of the full-length aciniform spidroin gene from Araneus ventricosus.
Int J Biol Macromol. 2018 Oct 01; 117:1352-1360.IJ

Abstract

Orb-web spiders produce more than seven different protein-based silks/glues by specialized abdominal glands for different uses. Prey-wrapping silk is secreted by aciniform glands for wrapping prey and forming the inner layer of egg case, and is almost twice as tough as other silks because of high strength and extensibility. So far, only two complete gene sequences have been obtained for aciniform spidroins (AcSp1). Here we describe the AcSp1 full-length gene sequence from the spider species Araneus ventricosus, using a long-distance PCR (LD-PCR) approach. The full-length AcSp1 gene is a single enormous exon of 10,338 bp in size, and the predicted protein sequence is 3445 amino acids long and consists of a conserved nonrepetitive N-terminal domain, a central predominantly repetitive region composed of fifteen repeat units, and a conserved nonrepetitive C-terminal domain.

Authors+Show Affiliations

Institute of Biological Sciences and Biotechnology, Donghua University, Shanghai, People's Republic of China.Institute of Biological Sciences and Biotechnology, Donghua University, Shanghai, People's Republic of China.Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada.Institute of Biological Sciences and Biotechnology, Donghua University, Shanghai, People's Republic of China.Institute of Biological Sciences and Biotechnology, Donghua University, Shanghai, People's Republic of China.Institute of Biological Sciences and Biotechnology, Donghua University, Shanghai, People's Republic of China. Electronic address: mengqing@dhu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29269010

Citation

Wen, Rui, et al. "Molecular Cloning and Analysis of the Full-length Aciniform Spidroin Gene From Araneus Ventricosus." International Journal of Biological Macromolecules, vol. 117, 2018, pp. 1352-1360.
Wen R, Wang K, Liu X, et al. Molecular cloning and analysis of the full-length aciniform spidroin gene from Araneus ventricosus. Int J Biol Macromol. 2018;117:1352-1360.
Wen, R., Wang, K., Liu, X., Li, X., Mi, J., & Meng, Q. (2018). Molecular cloning and analysis of the full-length aciniform spidroin gene from Araneus ventricosus. International Journal of Biological Macromolecules, 117, 1352-1360. https://doi.org/10.1016/j.ijbiomac.2017.12.090
Wen R, et al. Molecular Cloning and Analysis of the Full-length Aciniform Spidroin Gene From Araneus Ventricosus. Int J Biol Macromol. 2018 Oct 1;117:1352-1360. PubMed PMID: 29269010.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cloning and analysis of the full-length aciniform spidroin gene from Araneus ventricosus. AU - Wen,Rui, AU - Wang,Kangkang, AU - Liu,Xiangqin, AU - Li,Xue, AU - Mi,Junpeng, AU - Meng,Qing, Y1 - 2017/12/18/ PY - 2017/12/05/received PY - 2017/12/16/revised PY - 2017/12/16/accepted PY - 2017/12/23/pubmed PY - 2018/11/15/medline PY - 2017/12/23/entrez KW - Aciniform spidroin KW - Araneus ventricosus KW - Long-distance PCR SP - 1352 EP - 1360 JF - International journal of biological macromolecules JO - Int. J. Biol. Macromol. VL - 117 N2 - Orb-web spiders produce more than seven different protein-based silks/glues by specialized abdominal glands for different uses. Prey-wrapping silk is secreted by aciniform glands for wrapping prey and forming the inner layer of egg case, and is almost twice as tough as other silks because of high strength and extensibility. So far, only two complete gene sequences have been obtained for aciniform spidroins (AcSp1). Here we describe the AcSp1 full-length gene sequence from the spider species Araneus ventricosus, using a long-distance PCR (LD-PCR) approach. The full-length AcSp1 gene is a single enormous exon of 10,338 bp in size, and the predicted protein sequence is 3445 amino acids long and consists of a conserved nonrepetitive N-terminal domain, a central predominantly repetitive region composed of fifteen repeat units, and a conserved nonrepetitive C-terminal domain. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/29269010/Molecular_cloning_and_analysis_of_the_full_length_aciniform_spidroin_gene_from_Araneus_ventricosus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(17)34826-2 DB - PRIME DP - Unbound Medicine ER -