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Promising Prebiotic Candidate Established by Evaluation of Lactitol, Lactulose, Raffinose, and Oligofructose for Maintenance of a Lactobacillus-Dominated Vaginal Microbiota.
Appl Environ Microbiol. 2018 03 01; 84(5)AE

Abstract

Perturbations to the vaginal microbiota can lead to dysbiosis, including bacterial vaginosis (BV), which affects a large portion of the female population. In a healthy state, the vaginal microbiota is characterized by low diversity and colonization by Lactobacillus spp., whereas in BV, these species are displaced by a highly diverse population of bacteria associated with adverse vaginal health outcomes. Since prebiotic ingestion has been a highly effective approach to invigorate lactobacilli for improved intestinal health, we hypothesized that these compounds could stimulate lactobacilli at the expense of BV organisms to maintain vaginal health. Monocultures of commensal Lactobacillus crispatus, Lactobacillus vaginalis, Lactobacillus gasseri, Lactobacillus johnsonii, Lactobacillus jensenii, and Lactobacillus iners, in addition to BV-associated organisms and Candida albicans, were tested for their ability to utilize a representative group of prebiotics consisting of lactitol, lactulose, raffinose, and oligofructose. The disaccharide lactulose was found to most broadly and specifically stimulate vaginal lactobacilli, including the strongly health-associated species L. crispatus, and importantly, not to stimulate BV organisms or C. albicans Using freshly collected vaginal samples, we showed that exposure to lactulose promoted commensal Lactobacillus growth and dominance and resulted in healthy acidity partially through lactic acid production. This provides support for further testing of lactulose to prevent dysbiosis and potentially to reduce the need for antimicrobial agents in managing vaginal health.IMPORTANCE Bacterial vaginosis (BV) and other dysbioses of the vaginal microbiota significantly affect the quality of life of millions of women. Antimicrobial therapy is often poorly effective, causes side effects, and does not prevent recurrences. We report one of very few studies that have evaluated how prebiotics-compounds that are selectively fermented by beneficial bacteria such as Lactobacillus spp.-can modulate the vaginal microbiota. We also report use of a novel in vitro polymicrobial model to study the impact of prebiotics on the vaginal microbiota. The identification of prebiotic lactulose as enhancing Lactobacillus growth but not that of BV organisms or Candida albicans has direct application for retention of homeostasis and prevention of vaginal dysbiosis and infection.

Authors+Show Affiliations

Department of Microbiology and Immunology, The University of Western Ontario, London, Ontario, Canada. Canadian Centre for Human Microbiome and Probiotics, Lawson Health Research Institute, London, Ontario, Canada.Department of Microbiology and Immunology, The University of Western Ontario, London, Ontario, Canada. Canadian Centre for Human Microbiome and Probiotics, Lawson Health Research Institute, London, Ontario, Canada.Canadian Centre for Human Microbiome and Probiotics, Lawson Health Research Institute, London, Ontario, Canada.Public Health Laboratory, Public Health Service of Amsterdam (GGD), Amsterdam, The Netherlands.Molecular Cell Physiology, Faculty of Earth and Life Sciences, VU University, Amsterdam, The Netherlands. Netherlands Organisation for Applied Scientific Research (TNO), Microbiology and Systems Biology, Zeist, The Netherlands. Micropia, Natura Artis Magistra, Amsterdam, The Netherlands.Agriculture and Agri-Food Canada, London, Ontario, Canada.Department of Microbiology and Immunology, The University of Western Ontario, London, Ontario, Canada gregor@uwo.ca. Department of Surgery, The University of Western Ontario, London, Ontario, Canada. Canadian Centre for Human Microbiome and Probiotics, Lawson Health Research Institute, London, Ontario, Canada.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29269494

Citation

Collins, Stephanie L., et al. "Promising Prebiotic Candidate Established By Evaluation of Lactitol, Lactulose, Raffinose, and Oligofructose for Maintenance of a Lactobacillus-Dominated Vaginal Microbiota." Applied and Environmental Microbiology, vol. 84, no. 5, 2018.
Collins SL, McMillan A, Seney S, et al. Promising Prebiotic Candidate Established by Evaluation of Lactitol, Lactulose, Raffinose, and Oligofructose for Maintenance of a Lactobacillus-Dominated Vaginal Microbiota. Appl Environ Microbiol. 2018;84(5).
Collins, S. L., McMillan, A., Seney, S., van der Veer, C., Kort, R., Sumarah, M. W., & Reid, G. (2018). Promising Prebiotic Candidate Established by Evaluation of Lactitol, Lactulose, Raffinose, and Oligofructose for Maintenance of a Lactobacillus-Dominated Vaginal Microbiota. Applied and Environmental Microbiology, 84(5). https://doi.org/10.1128/AEM.02200-17
Collins SL, et al. Promising Prebiotic Candidate Established By Evaluation of Lactitol, Lactulose, Raffinose, and Oligofructose for Maintenance of a Lactobacillus-Dominated Vaginal Microbiota. Appl Environ Microbiol. 2018 03 1;84(5) PubMed PMID: 29269494.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Promising Prebiotic Candidate Established by Evaluation of Lactitol, Lactulose, Raffinose, and Oligofructose for Maintenance of a Lactobacillus-Dominated Vaginal Microbiota. AU - Collins,Stephanie L, AU - McMillan,Amy, AU - Seney,Shannon, AU - van der Veer,Charlotte, AU - Kort,Remco, AU - Sumarah,Mark W, AU - Reid,Gregor, Y1 - 2018/02/14/ PY - 2017/10/12/received PY - 2017/12/13/accepted PY - 2017/12/23/pubmed PY - 2019/2/12/medline PY - 2017/12/23/entrez KW - bacterial vaginosis KW - lactitol KW - lactobacilli KW - lactulose KW - oligofructose KW - prebiotics KW - raffinose KW - vaginal microbiota JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 84 IS - 5 N2 - Perturbations to the vaginal microbiota can lead to dysbiosis, including bacterial vaginosis (BV), which affects a large portion of the female population. In a healthy state, the vaginal microbiota is characterized by low diversity and colonization by Lactobacillus spp., whereas in BV, these species are displaced by a highly diverse population of bacteria associated with adverse vaginal health outcomes. Since prebiotic ingestion has been a highly effective approach to invigorate lactobacilli for improved intestinal health, we hypothesized that these compounds could stimulate lactobacilli at the expense of BV organisms to maintain vaginal health. Monocultures of commensal Lactobacillus crispatus, Lactobacillus vaginalis, Lactobacillus gasseri, Lactobacillus johnsonii, Lactobacillus jensenii, and Lactobacillus iners, in addition to BV-associated organisms and Candida albicans, were tested for their ability to utilize a representative group of prebiotics consisting of lactitol, lactulose, raffinose, and oligofructose. The disaccharide lactulose was found to most broadly and specifically stimulate vaginal lactobacilli, including the strongly health-associated species L. crispatus, and importantly, not to stimulate BV organisms or C. albicans Using freshly collected vaginal samples, we showed that exposure to lactulose promoted commensal Lactobacillus growth and dominance and resulted in healthy acidity partially through lactic acid production. This provides support for further testing of lactulose to prevent dysbiosis and potentially to reduce the need for antimicrobial agents in managing vaginal health.IMPORTANCE Bacterial vaginosis (BV) and other dysbioses of the vaginal microbiota significantly affect the quality of life of millions of women. Antimicrobial therapy is often poorly effective, causes side effects, and does not prevent recurrences. We report one of very few studies that have evaluated how prebiotics-compounds that are selectively fermented by beneficial bacteria such as Lactobacillus spp.-can modulate the vaginal microbiota. We also report use of a novel in vitro polymicrobial model to study the impact of prebiotics on the vaginal microbiota. The identification of prebiotic lactulose as enhancing Lactobacillus growth but not that of BV organisms or Candida albicans has direct application for retention of homeostasis and prevention of vaginal dysbiosis and infection. SN - 1098-5336 UR - https://www.unboundmedicine.com/medline/citation/29269494/Promising_Prebiotic_Candidate_Established_by_Evaluation_of_Lactitol_Lactulose_Raffinose_and_Oligofructose_for_Maintenance_of_a_Lactobacillus_Dominated_Vaginal_Microbiota_ L2 - http://aem.asm.org/lookup/pmidlookup?view=long&pmid=29269494 DB - PRIME DP - Unbound Medicine ER -