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Isolate Specificity and Polygenic Inheritance of Resistance in Barley to Diverse Heterologous Puccinia striiformis Isolates.
Phytopathology. 2018 May; 108(5):617-626.P

Abstract

Barley is a host to Puccinia striiformis f. sp. hordei, and is an intermediate or near nonhost to the formae speciales adapted to wheat (P. striiformis f. sp. tritici) and to barley grass (P. striiformis f. sp. pseudo-hordei). The genetic basis of resistance to these forms of P. striiformis is not well understood. Accordingly, a recombinant inbred line (RIL) population was developed using a P. striiformis-susceptible accession (Biosaline-19) and the immune cultivar Pompadour. We investigated the genetic basis of resistance to four diverse P. striiformis isolates (P. striiformis f. sp. pseudo-hordei, and P. striiformis f. sp. tritici pathotypes 104 E137 A-, 134 E16 A+, and 64 E0 A-). and determined that the immunity in Pompadour at the seedling stage to the different P. striiformis isolates was due to quantitative trait loci (QTL) on chromosomes 1H, 3H, 5H, and 7H with both overlapping and distinct specificities. Further histological analysis confirmed the presence of isolate specificity. The RILs were also assessed in the field for resistance to P. striiformis f. sp. pseudo-hordei, P. striiformis f. sp. hordei, and the leaf rust pathogen (P. hordei) to identify pleiotropic QTL loci effective at the adult plant stage and determine whether the leaf rust resistance in Pompadour (Rph20) was also effective to P. striiformis. RILs that were seedling susceptible to P. striiformis f. sp. pseudo-hordei were resistant in the field, implicating the involvement of adult plant resistance (APR). Additional QTLs were identified on chromosome 7H at the same genetic position as Rph23 (APR to leaf rust), suggesting either pleiotropic resistance or the presence of a stripe rust resistance gene closely linked to or allelic with Rph23. Unlike many pleiotropic APR genes identified and isolated in wheat, our data suggest that the Rph20 locus does not confer resistance to the P. striiformis isolates used in this study (P. striiformis f. sp. hordei [χ2 (independence) = 2.47 P > 0.12] and P. striiformis f. sp. pseudo-hordei [χ2 (independence) = 0.42 P > 0.60]).

Authors+Show Affiliations

First, second, fourth, and fifth authors: The University of Sydney, Plant Breeding Institute, Cobbitty, Private Bag 4011, Narellan, NSW, 2567, Australia; and second author: CSIRO Plant Industries, GPO Box 1600, Canberra, ACT, 2601, Australia.First, second, fourth, and fifth authors: The University of Sydney, Plant Breeding Institute, Cobbitty, Private Bag 4011, Narellan, NSW, 2567, Australia; and second author: CSIRO Plant Industries, GPO Box 1600, Canberra, ACT, 2601, Australia.First, second, fourth, and fifth authors: The University of Sydney, Plant Breeding Institute, Cobbitty, Private Bag 4011, Narellan, NSW, 2567, Australia; and second author: CSIRO Plant Industries, GPO Box 1600, Canberra, ACT, 2601, Australia.First, second, fourth, and fifth authors: The University of Sydney, Plant Breeding Institute, Cobbitty, Private Bag 4011, Narellan, NSW, 2567, Australia; and second author: CSIRO Plant Industries, GPO Box 1600, Canberra, ACT, 2601, Australia.First, second, fourth, and fifth authors: The University of Sydney, Plant Breeding Institute, Cobbitty, Private Bag 4011, Narellan, NSW, 2567, Australia; and second author: CSIRO Plant Industries, GPO Box 1600, Canberra, ACT, 2601, Australia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29271300

Citation

Haghdoust, R, et al. "Isolate Specificity and Polygenic Inheritance of Resistance in Barley to Diverse Heterologous Puccinia Striiformis Isolates." Phytopathology, vol. 108, no. 5, 2018, pp. 617-626.
Haghdoust R, Singh D, Garnica DP, et al. Isolate Specificity and Polygenic Inheritance of Resistance in Barley to Diverse Heterologous Puccinia striiformis Isolates. Phytopathology. 2018;108(5):617-626.
Haghdoust, R., Singh, D., Garnica, D. P., Park, R. F., & Dracatos, P. M. (2018). Isolate Specificity and Polygenic Inheritance of Resistance in Barley to Diverse Heterologous Puccinia striiformis Isolates. Phytopathology, 108(5), 617-626. https://doi.org/10.1094/PHYTO-10-17-0345-R
Haghdoust R, et al. Isolate Specificity and Polygenic Inheritance of Resistance in Barley to Diverse Heterologous Puccinia Striiformis Isolates. Phytopathology. 2018;108(5):617-626. PubMed PMID: 29271300.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolate Specificity and Polygenic Inheritance of Resistance in Barley to Diverse Heterologous Puccinia striiformis Isolates. AU - Haghdoust,R, AU - Singh,D, AU - Garnica,D P, AU - Park,R F, AU - Dracatos,P M, Y1 - 2018/03/21/ PY - 2017/12/23/pubmed PY - 2019/5/17/medline PY - 2017/12/23/entrez SP - 617 EP - 626 JF - Phytopathology JO - Phytopathology VL - 108 IS - 5 N2 - Barley is a host to Puccinia striiformis f. sp. hordei, and is an intermediate or near nonhost to the formae speciales adapted to wheat (P. striiformis f. sp. tritici) and to barley grass (P. striiformis f. sp. pseudo-hordei). The genetic basis of resistance to these forms of P. striiformis is not well understood. Accordingly, a recombinant inbred line (RIL) population was developed using a P. striiformis-susceptible accession (Biosaline-19) and the immune cultivar Pompadour. We investigated the genetic basis of resistance to four diverse P. striiformis isolates (P. striiformis f. sp. pseudo-hordei, and P. striiformis f. sp. tritici pathotypes 104 E137 A-, 134 E16 A+, and 64 E0 A-). and determined that the immunity in Pompadour at the seedling stage to the different P. striiformis isolates was due to quantitative trait loci (QTL) on chromosomes 1H, 3H, 5H, and 7H with both overlapping and distinct specificities. Further histological analysis confirmed the presence of isolate specificity. The RILs were also assessed in the field for resistance to P. striiformis f. sp. pseudo-hordei, P. striiformis f. sp. hordei, and the leaf rust pathogen (P. hordei) to identify pleiotropic QTL loci effective at the adult plant stage and determine whether the leaf rust resistance in Pompadour (Rph20) was also effective to P. striiformis. RILs that were seedling susceptible to P. striiformis f. sp. pseudo-hordei were resistant in the field, implicating the involvement of adult plant resistance (APR). Additional QTLs were identified on chromosome 7H at the same genetic position as Rph23 (APR to leaf rust), suggesting either pleiotropic resistance or the presence of a stripe rust resistance gene closely linked to or allelic with Rph23. Unlike many pleiotropic APR genes identified and isolated in wheat, our data suggest that the Rph20 locus does not confer resistance to the P. striiformis isolates used in this study (P. striiformis f. sp. hordei [χ2 (independence) = 2.47 P > 0.12] and P. striiformis f. sp. pseudo-hordei [χ2 (independence) = 0.42 P > 0.60]). SN - 0031-949X UR - https://www.unboundmedicine.com/medline/citation/29271300/Isolate_Specificity_and_Polygenic_Inheritance_of_Resistance_in_Barley_to_Diverse_Heterologous_Puccinia_striiformis_Isolates_ L2 - https://apsjournals.apsnet.org/doi/10.1094/PHYTO-10-17-0345-R?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -