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Combined effects of ZnO NPs and seawater acidification on the haemocyte parameters of thick shell mussel Mytilus coruscus.
Sci Total Environ. 2018 May 15; 624:820-830.ST

Abstract

Flow cytometry was used to investigate the immune parameters of haemocytes in thick-shell mussel Mytilus coruscus exposed to different concentrations of ZnO nanoparticles (NPs) (0, 2.5, and 10mgl-1) at two pH levels (7.3 and 8.1) for 14days following a recovery period of 7days. ZnO NPs significantly affected all of the immune parameters throughout the experiment. At high ZnO NPs concentrations, total haemocyte counting, phagocytosis, esterase, and lysosomal content were significantly decreased whereas haemocyte mortality and reactive oxygen species (ROS) were increased. Although low pH also significantly influenced all of the immune parameters of the mussels, its effect was not as strong as that of ZnO NPs. Interactive effects were observed between pH and ZnO NPs in most haemocyte parameters during the exposure period. Although a slight recovery from the stress of ZnO NPs and pH was observed for all immune parameters, significant carry-over effects of low pH and ZnO NPs were still detected. This study revealed that high concentration of ZnO NPs and low pH exert negative and synergistic effects on mussels, and these effects remain even after the mussels are no longer exposed to such stressors.

Authors+Show Affiliations

National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China.National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China.National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China.National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China.Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou, China.National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China.National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China.National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China.National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China.National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China; State Key Laboratory of Satellite Ocean Environment Dynamics, Second Institute of Oceanography, State Oceanic Administration, Hangzhou, China; Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou, China; State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200062, China. Electronic address: youjiwang2@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29274606

Citation

Wu, Fangli, et al. "Combined Effects of ZnO NPs and Seawater Acidification On the Haemocyte Parameters of Thick Shell Mussel Mytilus Coruscus." The Science of the Total Environment, vol. 624, 2018, pp. 820-830.
Wu F, Cui S, Sun M, et al. Combined effects of ZnO NPs and seawater acidification on the haemocyte parameters of thick shell mussel Mytilus coruscus. Sci Total Environ. 2018;624:820-830.
Wu, F., Cui, S., Sun, M., Xie, Z., Huang, W., Huang, X., Liu, L., Hu, M., Lu, W., & Wang, Y. (2018). Combined effects of ZnO NPs and seawater acidification on the haemocyte parameters of thick shell mussel Mytilus coruscus. The Science of the Total Environment, 624, 820-830. https://doi.org/10.1016/j.scitotenv.2017.12.168
Wu F, et al. Combined Effects of ZnO NPs and Seawater Acidification On the Haemocyte Parameters of Thick Shell Mussel Mytilus Coruscus. Sci Total Environ. 2018 May 15;624:820-830. PubMed PMID: 29274606.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Combined effects of ZnO NPs and seawater acidification on the haemocyte parameters of thick shell mussel Mytilus coruscus. AU - Wu,Fangli, AU - Cui,Shuaikang, AU - Sun,Meng, AU - Xie,Zhe, AU - Huang,Wei, AU - Huang,Xizhi, AU - Liu,Liping, AU - Hu,Menghong, AU - Lu,Weiqun, AU - Wang,Youji, Y1 - 2017/12/27/ PY - 2017/08/25/received PY - 2017/11/23/revised PY - 2017/12/15/accepted PY - 2017/12/24/pubmed PY - 2018/10/12/medline PY - 2017/12/24/entrez KW - Flow cytometry KW - Haemocyte KW - Immune response KW - Mussel KW - Nanoparticle KW - pH SP - 820 EP - 830 JF - The Science of the total environment JO - Sci Total Environ VL - 624 N2 - Flow cytometry was used to investigate the immune parameters of haemocytes in thick-shell mussel Mytilus coruscus exposed to different concentrations of ZnO nanoparticles (NPs) (0, 2.5, and 10mgl-1) at two pH levels (7.3 and 8.1) for 14days following a recovery period of 7days. ZnO NPs significantly affected all of the immune parameters throughout the experiment. At high ZnO NPs concentrations, total haemocyte counting, phagocytosis, esterase, and lysosomal content were significantly decreased whereas haemocyte mortality and reactive oxygen species (ROS) were increased. Although low pH also significantly influenced all of the immune parameters of the mussels, its effect was not as strong as that of ZnO NPs. Interactive effects were observed between pH and ZnO NPs in most haemocyte parameters during the exposure period. Although a slight recovery from the stress of ZnO NPs and pH was observed for all immune parameters, significant carry-over effects of low pH and ZnO NPs were still detected. This study revealed that high concentration of ZnO NPs and low pH exert negative and synergistic effects on mussels, and these effects remain even after the mussels are no longer exposed to such stressors. SN - 1879-1026 UR - https://www.unboundmedicine.com/medline/citation/29274606/Combined_effects_of_ZnO_NPs_and_seawater_acidification_on_the_haemocyte_parameters_of_thick_shell_mussel_Mytilus_coruscus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0048-9697(17)33594-5 DB - PRIME DP - Unbound Medicine ER -