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The NB-LRR gene Pm60 confers powdery mildew resistance in wheat.
New Phytol. 2018 04; 218(1):298-309.NP

Abstract

Powdery mildew is one of the most devastating diseases of wheat. To date, few powdery mildew resistance genes have been cloned from wheat due to the size and complexity of the wheat genome. Triticum urartu is the progenitor of the A genome of wheat and is an important source for powdery mildew resistance genes. Using molecular markers designed from scaffolds of the sequenced T. urartu accession and standard map-based cloning, a powdery mildew resistance locus was mapped to a 356-kb region, which contains two nucleotide-binding and leucine-rich repeat domain (NB-LRR) protein-encoding genes. Virus-induced gene silencing, single-cell transient expression, and stable transformation assays demonstrated that one of these two genes, designated Pm60, confers resistance to powdery mildew. Overexpression of full-length Pm60 and two allelic variants in Nicotiana benthamiana leaves induced hypersensitive cell death response, but expression of the coiled-coil domain alone was insufficient to induce hypersensitive response. Yeast two-hybrid, bimolecular fluorescence complementation and luciferase complementation imaging assays showed that Pm60 protein interacts with its neighboring NB-containing protein, suggesting that they might be functionally related. The identification and cloning of this novel wheat powdery mildew resistance gene will facilitate breeding for disease resistance in wheat.

Authors+Show Affiliations

State Key Laboratory of Ecological Control of Fujian-Taiwan Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Plant Immunity Center, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Development Biology, Chinese Academy of Sciences, Beijing, 100101, China. State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.State Key Laboratory of Ecological Control of Fujian-Taiwan Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Plant Immunity Center, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Development Biology, Chinese Academy of Sciences, Beijing, 100101, China. University of Chinese Academy of Sciences, Beijing, 100049, China.State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Development Biology, Chinese Academy of Sciences, Beijing, 100101, China.State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.State Key Laboratory of Ecological Control of Fujian-Taiwan Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Plant Immunity Center, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Development Biology, Chinese Academy of Sciences, Beijing, 100101, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29281751

Citation

Zou, Shenghao, et al. "The NB-LRR Gene Pm60 Confers Powdery Mildew Resistance in Wheat." The New Phytologist, vol. 218, no. 1, 2018, pp. 298-309.
Zou S, Wang H, Li Y, et al. The NB-LRR gene Pm60 confers powdery mildew resistance in wheat. New Phytol. 2018;218(1):298-309.
Zou, S., Wang, H., Li, Y., Kong, Z., & Tang, D. (2018). The NB-LRR gene Pm60 confers powdery mildew resistance in wheat. The New Phytologist, 218(1), 298-309. https://doi.org/10.1111/nph.14964
Zou S, et al. The NB-LRR Gene Pm60 Confers Powdery Mildew Resistance in Wheat. New Phytol. 2018;218(1):298-309. PubMed PMID: 29281751.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The NB-LRR gene Pm60 confers powdery mildew resistance in wheat. AU - Zou,Shenghao, AU - Wang,Huan, AU - Li,Yiwen, AU - Kong,Zhaosheng, AU - Tang,Dingzhong, Y1 - 2017/12/27/ PY - 2017/06/27/received PY - 2017/11/20/accepted PY - 2017/12/28/pubmed PY - 2019/9/29/medline PY - 2017/12/28/entrez KW - Triticum aestivum KW - Triticum urartu KW - Blumeria graminis f. sp. tritici (Bgt) KW - NB-LRR KW - Pm60 KW - powdery mildew KW - wheat SP - 298 EP - 309 JF - The New phytologist JO - New Phytol VL - 218 IS - 1 N2 - Powdery mildew is one of the most devastating diseases of wheat. To date, few powdery mildew resistance genes have been cloned from wheat due to the size and complexity of the wheat genome. Triticum urartu is the progenitor of the A genome of wheat and is an important source for powdery mildew resistance genes. Using molecular markers designed from scaffolds of the sequenced T. urartu accession and standard map-based cloning, a powdery mildew resistance locus was mapped to a 356-kb region, which contains two nucleotide-binding and leucine-rich repeat domain (NB-LRR) protein-encoding genes. Virus-induced gene silencing, single-cell transient expression, and stable transformation assays demonstrated that one of these two genes, designated Pm60, confers resistance to powdery mildew. Overexpression of full-length Pm60 and two allelic variants in Nicotiana benthamiana leaves induced hypersensitive cell death response, but expression of the coiled-coil domain alone was insufficient to induce hypersensitive response. Yeast two-hybrid, bimolecular fluorescence complementation and luciferase complementation imaging assays showed that Pm60 protein interacts with its neighboring NB-containing protein, suggesting that they might be functionally related. The identification and cloning of this novel wheat powdery mildew resistance gene will facilitate breeding for disease resistance in wheat. SN - 1469-8137 UR - https://www.unboundmedicine.com/medline/citation/29281751/The_NB_LRR_gene_Pm60_confers_powdery_mildew_resistance_in_wheat_ L2 - https://doi.org/10.1111/nph.14964 DB - PRIME DP - Unbound Medicine ER -