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Is comfrey root more than toxic pyrrolizidine alkaloids? Salvianolic acids among antioxidant polyphenols in comfrey (Symphytum officinale L.) roots.
Food Chem Toxicol 2018; 112:178-187FC

Abstract

Comfrey root preparations are used for the external treatment of joint distortions and myalgia, due to its analgesic and anti-inflammatory properties. Up to date, key activity-determining constituents of comfrey root extracts have not been completely elucidated. Therefore, we applied different approaches to further characterize a comfrey root extract (65% ethanol). The phenolic profile of comfrey root sample was characterized by HPLC-DAD-QTOF-MS/MS. Rosmarinic acid was identified as main phenolic constituent (7.55 mg/g extract). Moreover, trimers and tetramers of caffeic acid (isomers of salvianolic acid A, B and C) were identified and quantified for the first time in comfrey root. In addition, pyrrolizidine alkaloids were evaluated by HPLC-QQQ-MS/MS and acetylintermedine, acetyllycopsamine and their N-oxides were determined as major pyrrolizidine alkaloids in the comfrey root sample. Lastly, the antioxidant activity was determined using four assays: DPPH and ABTS radicals scavenging assays, reducing power assay and 15-lipoxygenase inhibition assay. Comfrey root extract exhibited significant antioxidant activities when compared to known antioxidants. Thus, comfrey root is an important source of phenolic compounds endowed with antioxidant activity which may contribute to the overall bioactivity of Symphytum preparations.

Authors+Show Affiliations

Department of Pharmacognosy, Faculty of Pharmacy, Grigore T. Popa University of Medicine and Pharmacy Iasi, Iasi 700115, Romania; Phytopharmacy and Natural Products Research Group, Institute of Chemistry and Biotechnology, Zurich University of Applied Sciences, Wädenswil 8820, Switzerland. Electronic address: adriana.trifan@umfiasi.ro.Analytical Technologies Group, Institute of Chemistry and Biotechnology, Zurich University of Applied Sciences, Wädenswil 8820, Switzerland.Centre for Biochemistry and Bioanalytics, Institute of Chemistry and Biotechnology, Zurich University of Applied Sciences, Wädenswil 8820, Switzerland.Alpinamed AG, Freidorf 9306, Switzerland.Alpinamed AG, Freidorf 9306, Switzerland.Phytopharmacy and Natural Products Research Group, Institute of Chemistry and Biotechnology, Zurich University of Applied Sciences, Wädenswil 8820, Switzerland.Phytopharmacy and Natural Products Research Group, Institute of Chemistry and Biotechnology, Zurich University of Applied Sciences, Wädenswil 8820, Switzerland.Phytopharmacy and Natural Products Research Group, Institute of Chemistry and Biotechnology, Zurich University of Applied Sciences, Wädenswil 8820, Switzerland.Phytopharmacy and Natural Products Research Group, Institute of Chemistry and Biotechnology, Zurich University of Applied Sciences, Wädenswil 8820, Switzerland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29288756

Citation

Trifan, Adriana, et al. "Is Comfrey Root More Than Toxic Pyrrolizidine Alkaloids? Salvianolic Acids Among Antioxidant Polyphenols in Comfrey (Symphytum Officinale L.) Roots." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 112, 2018, pp. 178-187.
Trifan A, Opitz SEW, Josuran R, et al. Is comfrey root more than toxic pyrrolizidine alkaloids? Salvianolic acids among antioxidant polyphenols in comfrey (Symphytum officinale L.) roots. Food Chem Toxicol. 2018;112:178-187.
Trifan, A., Opitz, S. E. W., Josuran, R., Grubelnik, A., Esslinger, N., Peter, S., ... Wolfram, E. (2018). Is comfrey root more than toxic pyrrolizidine alkaloids? Salvianolic acids among antioxidant polyphenols in comfrey (Symphytum officinale L.) roots. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 112, pp. 178-187. doi:10.1016/j.fct.2017.12.051.
Trifan A, et al. Is Comfrey Root More Than Toxic Pyrrolizidine Alkaloids? Salvianolic Acids Among Antioxidant Polyphenols in Comfrey (Symphytum Officinale L.) Roots. Food Chem Toxicol. 2018;112:178-187. PubMed PMID: 29288756.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Is comfrey root more than toxic pyrrolizidine alkaloids? Salvianolic acids among antioxidant polyphenols in comfrey (Symphytum officinale L.) roots. AU - Trifan,Adriana, AU - Opitz,Sebastian E W, AU - Josuran,Roland, AU - Grubelnik,Andreas, AU - Esslinger,Nils, AU - Peter,Samuel, AU - Bräm,Sarah, AU - Meier,Nadja, AU - Wolfram,Evelyn, Y1 - 2017/12/28/ PY - 2017/11/03/received PY - 2017/12/21/revised PY - 2017/12/22/accepted PY - 2017/12/31/pubmed PY - 2018/10/4/medline PY - 2017/12/31/entrez KW - Antioxidant activity KW - Phenolic compounds KW - Pyrrolizidine alkaloids KW - Salvianolic acids KW - Symphytum officinale L. SP - 178 EP - 187 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem. Toxicol. VL - 112 N2 - Comfrey root preparations are used for the external treatment of joint distortions and myalgia, due to its analgesic and anti-inflammatory properties. Up to date, key activity-determining constituents of comfrey root extracts have not been completely elucidated. Therefore, we applied different approaches to further characterize a comfrey root extract (65% ethanol). The phenolic profile of comfrey root sample was characterized by HPLC-DAD-QTOF-MS/MS. Rosmarinic acid was identified as main phenolic constituent (7.55 mg/g extract). Moreover, trimers and tetramers of caffeic acid (isomers of salvianolic acid A, B and C) were identified and quantified for the first time in comfrey root. In addition, pyrrolizidine alkaloids were evaluated by HPLC-QQQ-MS/MS and acetylintermedine, acetyllycopsamine and their N-oxides were determined as major pyrrolizidine alkaloids in the comfrey root sample. Lastly, the antioxidant activity was determined using four assays: DPPH and ABTS radicals scavenging assays, reducing power assay and 15-lipoxygenase inhibition assay. Comfrey root extract exhibited significant antioxidant activities when compared to known antioxidants. Thus, comfrey root is an important source of phenolic compounds endowed with antioxidant activity which may contribute to the overall bioactivity of Symphytum preparations. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/29288756/Is_comfrey_root_more_than_toxic_pyrrolizidine_alkaloids_Salvianolic_acids_among_antioxidant_polyphenols_in_comfrey__Symphytum_officinale_L___roots_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(17)30799-8 DB - PRIME DP - Unbound Medicine ER -