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Fluorescence in situ hybridization karyotyping reveals the presence of two distinct genomes in the taxon Aegilops tauschii.
BMC Genomics. 2018 01 02; 19(1):3.BG

Abstract

BACKGROUND

Aegilops tauschii is the donor of the bread wheat D genome. Based on spike morphology, the taxon has conventionally been subdivided into ssp. tauschii and ssp. strangulata. The present study was intended to address the poor match between this whole plant morphology-based subdivision and genetic relationships inferred from genotyping by fluorescence in situ hybridization karyotyping a set of 31 Ae. tauschii accessions.

RESULTS

The distribution of sites hybridizing to the two probes oligo-pTa-535 and (CTT)10 split the Ae. tauschii accessions into two clades, designated Dt and Ds, which corresponded perfectly with a previously assembled phylogeny based on marker genotype. The Dt cluster was populated exclusively by ssp. tauschii accessions, while the Ds cluster harbored both ssp. strangulata and morphologically intermediate accessions. As a result, it is proposed that Ae. tauschii ssp. tauschii is restricted to carriers of the Dt karyotype: their spikelets are regularly spaced along the rachis, at least in the central portion of their spike. Accessions classified as Ae. tauschii ssp. strangulata carry the Ds karyotype; their spikelets are irregularly spaced. Based on this criterion, forms formerly classified as ssp. tauschii var. meyeri have been re-designated ssp. strangulata var. meyeri.

CONCLUSIONS

According to the reworking of the taxon, the bread wheat D genome was most probably donated by ssp. strangulata var. meyeri. Chromosomal differentiation reveals intra-species taxon of Ae. tauschii. Ae. tauschii ssp. tauschii has more distant relationship with breed wheat than ssp. strangulata and can be used for breeding improving effectively.

Authors+Show Affiliations

Triticeae Research Institute, Sichuan Agricultural University, No. 211 Huiming Rd, Wenjiang District, Chengdu City, Sichuan province, 611130, People's Republic of China.Triticeae Research Institute, Sichuan Agricultural University, No. 211 Huiming Rd, Wenjiang District, Chengdu City, Sichuan province, 611130, People's Republic of China.Triticeae Research Institute, Sichuan Agricultural University, No. 211 Huiming Rd, Wenjiang District, Chengdu City, Sichuan province, 611130, People's Republic of China.Triticeae Research Institute, Sichuan Agricultural University, No. 211 Huiming Rd, Wenjiang District, Chengdu City, Sichuan province, 611130, People's Republic of China.Triticeae Research Institute, Sichuan Agricultural University, No. 211 Huiming Rd, Wenjiang District, Chengdu City, Sichuan province, 611130, People's Republic of China.Triticeae Research Institute, Sichuan Agricultural University, No. 211 Huiming Rd, Wenjiang District, Chengdu City, Sichuan province, 611130, People's Republic of China. Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Yaan, Sichuan, 625014, China.Triticeae Research Institute, Sichuan Agricultural University, No. 211 Huiming Rd, Wenjiang District, Chengdu City, Sichuan province, 611130, People's Republic of China. Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Yaan, Sichuan, 625014, China.Triticeae Research Institute, Sichuan Agricultural University, No. 211 Huiming Rd, Wenjiang District, Chengdu City, Sichuan province, 611130, People's Republic of China. haomingluo@foxmail.com.Triticeae Research Institute, Sichuan Agricultural University, No. 211 Huiming Rd, Wenjiang District, Chengdu City, Sichuan province, 611130, People's Republic of China. dcliu7@yahoo.com. Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Yaan, Sichuan, 625014, China. dcliu7@yahoo.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29291709

Citation

Zhao, Laibin, et al. "Fluorescence in Situ Hybridization Karyotyping Reveals the Presence of Two Distinct Genomes in the Taxon Aegilops Tauschii." BMC Genomics, vol. 19, no. 1, 2018, p. 3.
Zhao L, Ning S, Yi Y, et al. Fluorescence in situ hybridization karyotyping reveals the presence of two distinct genomes in the taxon Aegilops tauschii. BMC Genomics. 2018;19(1):3.
Zhao, L., Ning, S., Yi, Y., Zhang, L., Yuan, Z., Wang, J., Zheng, Y., Hao, M., & Liu, D. (2018). Fluorescence in situ hybridization karyotyping reveals the presence of two distinct genomes in the taxon Aegilops tauschii. BMC Genomics, 19(1), 3. https://doi.org/10.1186/s12864-017-4384-0
Zhao L, et al. Fluorescence in Situ Hybridization Karyotyping Reveals the Presence of Two Distinct Genomes in the Taxon Aegilops Tauschii. BMC Genomics. 2018 01 2;19(1):3. PubMed PMID: 29291709.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fluorescence in situ hybridization karyotyping reveals the presence of two distinct genomes in the taxon Aegilops tauschii. AU - Zhao,Laibin, AU - Ning,Shunzong, AU - Yi,Yingjin, AU - Zhang,Lianquan, AU - Yuan,Zhongwei, AU - Wang,Jirui, AU - Zheng,Youliang, AU - Hao,Ming, AU - Liu,Dengcai, Y1 - 2018/01/02/ PY - 2017/06/06/received PY - 2017/12/15/accepted PY - 2018/1/3/entrez PY - 2018/1/3/pubmed PY - 2018/7/7/medline KW - Chromosome differentiation KW - D genome KW - Repeat sequences KW - Spike morphology KW - Subspecies KW - Wheat evolution SP - 3 EP - 3 JF - BMC genomics JO - BMC Genomics VL - 19 IS - 1 N2 - BACKGROUND: Aegilops tauschii is the donor of the bread wheat D genome. Based on spike morphology, the taxon has conventionally been subdivided into ssp. tauschii and ssp. strangulata. The present study was intended to address the poor match between this whole plant morphology-based subdivision and genetic relationships inferred from genotyping by fluorescence in situ hybridization karyotyping a set of 31 Ae. tauschii accessions. RESULTS: The distribution of sites hybridizing to the two probes oligo-pTa-535 and (CTT)10 split the Ae. tauschii accessions into two clades, designated Dt and Ds, which corresponded perfectly with a previously assembled phylogeny based on marker genotype. The Dt cluster was populated exclusively by ssp. tauschii accessions, while the Ds cluster harbored both ssp. strangulata and morphologically intermediate accessions. As a result, it is proposed that Ae. tauschii ssp. tauschii is restricted to carriers of the Dt karyotype: their spikelets are regularly spaced along the rachis, at least in the central portion of their spike. Accessions classified as Ae. tauschii ssp. strangulata carry the Ds karyotype; their spikelets are irregularly spaced. Based on this criterion, forms formerly classified as ssp. tauschii var. meyeri have been re-designated ssp. strangulata var. meyeri. CONCLUSIONS: According to the reworking of the taxon, the bread wheat D genome was most probably donated by ssp. strangulata var. meyeri. Chromosomal differentiation reveals intra-species taxon of Ae. tauschii. Ae. tauschii ssp. tauschii has more distant relationship with breed wheat than ssp. strangulata and can be used for breeding improving effectively. SN - 1471-2164 UR - https://www.unboundmedicine.com/medline/citation/29291709/Fluorescence_in_situ_hybridization_karyotyping_reveals_the_presence_of_two_distinct_genomes_in_the_taxon_Aegilops_tauschii_ L2 - https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-017-4384-0 DB - PRIME DP - Unbound Medicine ER -