Tags

Type your tag names separated by a space and hit enter

Effect of secondary organic aerosol from isoprene-derived hydroxyhydroperoxides on the expression of oxidative stress response genes in human bronchial epithelial cells.
Environ Sci Process Impacts. 2018 Feb 21; 20(2):332-339.ES

Abstract

Isoprene-derived secondary organic aerosol (SOA), which comprise a large portion of atmospheric fine particulate matter (PM2.5), can be formed through various gaseous precursors, including isoprene epoxydiols (IEPOX), methacrylic acid epoxide (MAE), and isoprene hydroxyhydroperoxides (ISOPOOH). The composition of the isoprene-derived SOA affects its reactive oxygen species (ROS) generation potential and its ability to alter oxidative stress-related gene expression. In this study we assess effects of isoprene SOA derived solely from ISOPOOH oxidation on human bronchial epithelial cells by measuring the gene expression changes in 84 oxidative stress-related genes. In addition, the thiol reactivity of ISOPOOH-derived SOA was measured through the dithiothreitol (DTT) assay. Our findings show that ISOPOOH-derived SOA alter more oxidative-stress related genes than IEPOX-derived SOA but not as many as MAE-derived SOA on a mass basis exposure. More importantly, we found that the different types of SOA derived from the various gaseous precursors (MAE, IEPOX, and ISOPOOH) have unique contributions to changes in oxidative stress-related genes that do not total all gene expression changes seen in exposures to atmospherically relevant compositions of total isoprene-derived SOA mixtures. This study suggests that amongst the different types of known isoprene-derived SOA, MAE-derived SOA are the most potent inducer of oxidative stress-related gene changes but highlights the importance of considering isoprene-derived SOA as a total mixture for pollution controls and exposure studies.

Authors+Show Affiliations

Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. surratt@unc.edu.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29292423

Citation

Arashiro, Maiko, et al. "Effect of Secondary Organic Aerosol From Isoprene-derived Hydroxyhydroperoxides On the Expression of Oxidative Stress Response Genes in Human Bronchial Epithelial Cells." Environmental Science. Processes & Impacts, vol. 20, no. 2, 2018, pp. 332-339.
Arashiro M, Lin YH, Zhang Z, et al. Effect of secondary organic aerosol from isoprene-derived hydroxyhydroperoxides on the expression of oxidative stress response genes in human bronchial epithelial cells. Environ Sci Process Impacts. 2018;20(2):332-339.
Arashiro, M., Lin, Y. H., Zhang, Z., Sexton, K. G., Gold, A., Jaspers, I., Fry, R. C., & Surratt, J. D. (2018). Effect of secondary organic aerosol from isoprene-derived hydroxyhydroperoxides on the expression of oxidative stress response genes in human bronchial epithelial cells. Environmental Science. Processes & Impacts, 20(2), 332-339. https://doi.org/10.1039/c7em00439g
Arashiro M, et al. Effect of Secondary Organic Aerosol From Isoprene-derived Hydroxyhydroperoxides On the Expression of Oxidative Stress Response Genes in Human Bronchial Epithelial Cells. Environ Sci Process Impacts. 2018 Feb 21;20(2):332-339. PubMed PMID: 29292423.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of secondary organic aerosol from isoprene-derived hydroxyhydroperoxides on the expression of oxidative stress response genes in human bronchial epithelial cells. AU - Arashiro,Maiko, AU - Lin,Ying-Hsuan, AU - Zhang,Zhenfa, AU - Sexton,Kenneth G, AU - Gold,Avram, AU - Jaspers,Ilona, AU - Fry,Rebecca C, AU - Surratt,Jason D, PY - 2018/1/3/pubmed PY - 2018/6/5/medline PY - 2018/1/3/entrez SP - 332 EP - 339 JF - Environmental science. Processes & impacts JO - Environ Sci Process Impacts VL - 20 IS - 2 N2 - Isoprene-derived secondary organic aerosol (SOA), which comprise a large portion of atmospheric fine particulate matter (PM2.5), can be formed through various gaseous precursors, including isoprene epoxydiols (IEPOX), methacrylic acid epoxide (MAE), and isoprene hydroxyhydroperoxides (ISOPOOH). The composition of the isoprene-derived SOA affects its reactive oxygen species (ROS) generation potential and its ability to alter oxidative stress-related gene expression. In this study we assess effects of isoprene SOA derived solely from ISOPOOH oxidation on human bronchial epithelial cells by measuring the gene expression changes in 84 oxidative stress-related genes. In addition, the thiol reactivity of ISOPOOH-derived SOA was measured through the dithiothreitol (DTT) assay. Our findings show that ISOPOOH-derived SOA alter more oxidative-stress related genes than IEPOX-derived SOA but not as many as MAE-derived SOA on a mass basis exposure. More importantly, we found that the different types of SOA derived from the various gaseous precursors (MAE, IEPOX, and ISOPOOH) have unique contributions to changes in oxidative stress-related genes that do not total all gene expression changes seen in exposures to atmospherically relevant compositions of total isoprene-derived SOA mixtures. This study suggests that amongst the different types of known isoprene-derived SOA, MAE-derived SOA are the most potent inducer of oxidative stress-related gene changes but highlights the importance of considering isoprene-derived SOA as a total mixture for pollution controls and exposure studies. SN - 2050-7895 UR - https://www.unboundmedicine.com/medline/citation/29292423/Effect_of_secondary_organic_aerosol_from_isoprene_derived_hydroxyhydroperoxides_on_the_expression_of_oxidative_stress_response_genes_in_human_bronchial_epithelial_cells_ L2 - https://doi.org/10.1039/c7em00439g DB - PRIME DP - Unbound Medicine ER -