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[Mono-carbonyl analogues of curcumin prevents paraquat-induced apoptosis in HK-2 cell line by inhibiting oxidative damage and inflammation].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2017 Sep 20; 35(9):641-647.ZL

Abstract

Objective:

To investigate the effects of mono-carbonyl analogues of curcumin (L6H21) on paraquat (PQ) -induced injury in HK-2 cell line and explore its underlying mechanisms.

Methods:

Cultured HK-2 cells were challenged by PQ with or without L6H21 treatment. Cell viability and apoptosis were determined by CCK-8 assay and flow cytometry, respectively. Gene expressions and protein levels of apoptotic and inflammatory factors were assessed by RT-PCR, ELISA, and western blot. Intracellular ROS production was detected by DCFH-DA staining. Superoxide dismutase (SOD) and malondialdehyde (MDA) were examined by chemical colorimetry.

Results:

1) PQ challenge significantly inhibited HK-2 cells proliferation, which was prevented by L6H21 administration. PQ dramatically induced HK-2 apoptosis evidenced by increasing expressions of caspase-9, caspase-3 and Bax, while decreasing Bcl-2 level. However, PQ induced these apoptotic effects in HK-2 cells were reversed by L6H21. Similarly, PQ exposure obviously enhanced activity of NF-κB and levels of cytokines (TNF-α、IL-6) in HK-2 cells, which was inhibited by L6H21. Furthermore, administration of L6H21 inhibited PQ induced ROS and MDA production, and promoted SOD level in HK-2 cells.

Conclusion:

L6H21 administration inhibits PQ-induced apoptosis in HK-2 cells possibly by reducing inflammation and oxidative damage.

Authors+Show Affiliations

Emergency Department of the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

29294513

Citation

Hong, G L., et al. "[Mono-carbonyl Analogues of Curcumin Prevents Paraquat-induced Apoptosis in HK-2 Cell Line By Inhibiting Oxidative Damage and Inflammation]." Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi = Zhonghua Laodong Weisheng Zhiyebing Zazhi = Chinese Journal of Industrial Hygiene and Occupational Diseases, vol. 35, no. 9, 2017, pp. 641-647.
Hong GL, Yang ZN, He YY, et al. [Mono-carbonyl analogues of curcumin prevents paraquat-induced apoptosis in HK-2 cell line by inhibiting oxidative damage and inflammation]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2017;35(9):641-647.
Hong, G. L., Yang, Z. N., He, Y. Y., Tan, J. P., Liangm, A., Zhao, G. J., & Lu, Z. Q. (2017). [Mono-carbonyl analogues of curcumin prevents paraquat-induced apoptosis in HK-2 cell line by inhibiting oxidative damage and inflammation]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi = Zhonghua Laodong Weisheng Zhiyebing Zazhi = Chinese Journal of Industrial Hygiene and Occupational Diseases, 35(9), 641-647. https://doi.org/10.3760/cma.j.issn.1001-9391.2017.09.001
Hong GL, et al. [Mono-carbonyl Analogues of Curcumin Prevents Paraquat-induced Apoptosis in HK-2 Cell Line By Inhibiting Oxidative Damage and Inflammation]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2017 Sep 20;35(9):641-647. PubMed PMID: 29294513.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Mono-carbonyl analogues of curcumin prevents paraquat-induced apoptosis in HK-2 cell line by inhibiting oxidative damage and inflammation]. AU - Hong,G L, AU - Yang,Z N, AU - He,Y Y, AU - Tan,J P, AU - Liangm,A, AU - Zhao,G J, AU - Lu,Z Q, PY - 2018/1/3/entrez PY - 2018/1/4/pubmed PY - 2018/10/17/medline KW - Curcumin KW - Epithelial cell KW - Mono-carbonyl analogues of curcumin KW - Paraquat SP - 641 EP - 647 JF - Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases JO - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi VL - 35 IS - 9 N2 - Objective: To investigate the effects of mono-carbonyl analogues of curcumin (L6H21) on paraquat (PQ) -induced injury in HK-2 cell line and explore its underlying mechanisms. Methods: Cultured HK-2 cells were challenged by PQ with or without L6H21 treatment. Cell viability and apoptosis were determined by CCK-8 assay and flow cytometry, respectively. Gene expressions and protein levels of apoptotic and inflammatory factors were assessed by RT-PCR, ELISA, and western blot. Intracellular ROS production was detected by DCFH-DA staining. Superoxide dismutase (SOD) and malondialdehyde (MDA) were examined by chemical colorimetry. Results: 1) PQ challenge significantly inhibited HK-2 cells proliferation, which was prevented by L6H21 administration. PQ dramatically induced HK-2 apoptosis evidenced by increasing expressions of caspase-9, caspase-3 and Bax, while decreasing Bcl-2 level. However, PQ induced these apoptotic effects in HK-2 cells were reversed by L6H21. Similarly, PQ exposure obviously enhanced activity of NF-κB and levels of cytokines (TNF-α、IL-6) in HK-2 cells, which was inhibited by L6H21. Furthermore, administration of L6H21 inhibited PQ induced ROS and MDA production, and promoted SOD level in HK-2 cells. Conclusion: L6H21 administration inhibits PQ-induced apoptosis in HK-2 cells possibly by reducing inflammation and oxidative damage. SN - 1001-9391 UR - https://www.unboundmedicine.com/medline/citation/29294513/[Mono_carbonyl_analogues_of_curcumin_prevents_paraquat_induced_apoptosis_in_HK_2_cell_line_by_inhibiting_oxidative_damage_and_inflammation]_ L2 - https://antibodies.cancer.gov/detail/CPTC-CASP3-1 DB - PRIME DP - Unbound Medicine ER -