Tags

Type your tag names separated by a space and hit enter

Identification of Immunoreactive Peptides of Toxins to Simultaneously Assess the Neutralization Potency of Antivenoms against Neurotoxicity and Cytotoxicity of Naja atra Venom.
Toxins (Basel). 2017 12 25; 10(1)T

Abstract

Assessing the neutralization capability of nonlethal but medically relevant toxins in venom has been a challenging task. Nowadays, neutralization efficacy is evaluated based simply on the survival rates of animals injected with antivenom together with a predefined dose of venom, which can determine potency against neurotoxicity but not validate the capability to neutralize cytotoxin-induced complications. In this study, a high correlation with in-vivo and in-vitro neutralization assays was established using the immunoreactive peptides identified from short-chain neurotoxin and cytotoxin A3. These peptides contain conserved residues associated with toxin activities and a competition assay indicated that these peptides could specifically block the antibody binding to toxin and affect the neutralization potency of antivenom. Moreover, the titers of peptide-specific antibody in antivenoms or mouse antisera were determined by enzyme-linked immunosorbent assay (ELISA) simultaneously, and the results indicated that Taiwanese bivalent antivenom (BAV) and Vietnamese snake antivenom-Naja (SAV-Naja) exhibited superior neutralization potency against the lethal effect of short-chain neurotoxin (sNTX) and cytotoxicity of cardiotoxin/cytotoxin (CTX), respectively. Thus, the reported peptide ELISA shows not only its potential for antivenom prequalification use, but also its capability of justifying the cross-neutralization potency of antivenoms against Naja atra venom toxicity.

Authors+Show Affiliations

National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli 35053, Taiwan. scwu@mx.nthu.edu.tw. Institute of Biotechnology, Department of Medical Science, National Tsing Hua University, Hsinchu 30013, Taiwan. scwu@mx.nthu.edu.tw.Institute of Bioinformatics and Structural Biology, Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan. lswwg@life.nthu.edu.tw.National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli 35053, Taiwan. bingsin@nhri.org.tw.National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli 35053, Taiwan. hamlyn@nhri.org.tw.National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli 35053, Taiwan. han@nhri.org.tw.Institute of Biotechnology, Department of Medical Science, National Tsing Hua University, Hsinchu 30013, Taiwan.National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli 35053, Taiwan. jiangbj@nhri.org.tw.National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli 35053, Taiwan. sung23@nhri.org.tw.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29295601

Citation

Liu, Bin-Sin, et al. "Identification of Immunoreactive Peptides of Toxins to Simultaneously Assess the Neutralization Potency of Antivenoms Against Neurotoxicity and Cytotoxicity of Naja Atra Venom." Toxins, vol. 10, no. 1, 2017.
Liu BS, Wu WG, Lin MH, et al. Identification of Immunoreactive Peptides of Toxins to Simultaneously Assess the Neutralization Potency of Antivenoms against Neurotoxicity and Cytotoxicity of Naja atra Venom. Toxins (Basel). 2017;10(1).
Liu, B. S., Wu, W. G., Lin, M. H., Li, C. H., Jiang, B. R., Wu, S. C., Leng, C. H., & Sung, W. C. (2017). Identification of Immunoreactive Peptides of Toxins to Simultaneously Assess the Neutralization Potency of Antivenoms against Neurotoxicity and Cytotoxicity of Naja atra Venom. Toxins, 10(1). https://doi.org/10.3390/toxins10010010
Liu BS, et al. Identification of Immunoreactive Peptides of Toxins to Simultaneously Assess the Neutralization Potency of Antivenoms Against Neurotoxicity and Cytotoxicity of Naja Atra Venom. Toxins (Basel). 2017 12 25;10(1) PubMed PMID: 29295601.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of Immunoreactive Peptides of Toxins to Simultaneously Assess the Neutralization Potency of Antivenoms against Neurotoxicity and Cytotoxicity of Naja atra Venom. AU - Liu,Bin-Sin, AU - Wu,Wen-Guey, AU - Lin,Min-Han, AU - Li,Chi-Han, AU - Jiang,Bo-Rong, AU - Wu,Suh-Chin, AU - Leng,Chih-Hsiang, AU - Sung,Wang-Chou, Y1 - 2017/12/25/ PY - 2017/11/08/received PY - 2017/12/21/revised PY - 2017/12/22/accepted PY - 2018/1/4/entrez PY - 2018/1/4/pubmed PY - 2018/12/24/medline KW - ELISA KW - Naja atra KW - antivenom KW - assessment KW - immunoreactive peptide KW - neutralization JF - Toxins JO - Toxins (Basel) VL - 10 IS - 1 N2 - Assessing the neutralization capability of nonlethal but medically relevant toxins in venom has been a challenging task. Nowadays, neutralization efficacy is evaluated based simply on the survival rates of animals injected with antivenom together with a predefined dose of venom, which can determine potency against neurotoxicity but not validate the capability to neutralize cytotoxin-induced complications. In this study, a high correlation with in-vivo and in-vitro neutralization assays was established using the immunoreactive peptides identified from short-chain neurotoxin and cytotoxin A3. These peptides contain conserved residues associated with toxin activities and a competition assay indicated that these peptides could specifically block the antibody binding to toxin and affect the neutralization potency of antivenom. Moreover, the titers of peptide-specific antibody in antivenoms or mouse antisera were determined by enzyme-linked immunosorbent assay (ELISA) simultaneously, and the results indicated that Taiwanese bivalent antivenom (BAV) and Vietnamese snake antivenom-Naja (SAV-Naja) exhibited superior neutralization potency against the lethal effect of short-chain neurotoxin (sNTX) and cytotoxicity of cardiotoxin/cytotoxin (CTX), respectively. Thus, the reported peptide ELISA shows not only its potential for antivenom prequalification use, but also its capability of justifying the cross-neutralization potency of antivenoms against Naja atra venom toxicity. SN - 2072-6651 UR - https://www.unboundmedicine.com/medline/citation/29295601/Identification_of_Immunoreactive_Peptides_of_Toxins_to_Simultaneously_Assess_the_Neutralization_Potency_of_Antivenoms_against_Neurotoxicity_and_Cytotoxicity_of_Naja_atra_Venom_ L2 - https://www.mdpi.com/resolver?pii=toxins10010010 DB - PRIME DP - Unbound Medicine ER -