Tags

Type your tag names separated by a space and hit enter

Toxicology of Gambierdiscus spp. (Dinophyceae) from Tropical and Temperate Australian Waters.
Mar Drugs. 2018 Jan 01; 16(1)MD

Abstract

Ciguatera Fish Poisoning (CFP) is a human illness caused by the consumption of marine fish contaminated with ciguatoxins (CTX) and possibly maitotoxins (MTX), produced by species from the benthic dinoflagellate genus Gambierdiscus. Here, we describe the identity and toxicology of Gambierdiscus spp. isolated from the tropical and temperate waters of eastern Australia. Based on newly cultured strains, we found that four Gambierdiscus species were present at the tropical location, including G. carpenteri, G. lapillus and two others which were not genetically identical to other currently described species within the genus, and may represent new species. Only G. carpenteri was identified from the temperate location. Using LC-MS/MS analysis we did not find any characterized microalgal CTXs (P-CTX-3B, P-CTX-3C, P-CTX-4A and P-CTX-4B) or MTX-1; however, putative maitotoxin-3 (MTX-3) was detected in all species except for the temperate population of G. carpenteri. Using the Ca2+ influx SH-SY5Y cell Fluorescent Imaging Plate Reader (FLIPR) bioassay we found CTX-like activity in extracts of the unidentified Gambierdiscus strains and trace level activity in strains of G. lapillus. While no detectable CTX-like activity was observed in tropical or temperate strains of G. carpenteri, all species showed strong maitotoxin-like activity. This study, which represents the most comprehensive analyses of the toxicology of Gambierdiscus strains isolated from Australia to date, suggests that CFP in this region may be caused by currently undescribed ciguatoxins and maitotoxins.

Authors+Show Affiliations

Climate Change Cluster, University of Technology Sydney, P.O. Box 123 Broadway, Sydney, NSW 2007, Australia. Michaela.Larsson@uts.edu.au.Climate Change Cluster, University of Technology Sydney, P.O. Box 123 Broadway, Sydney, NSW 2007, Australia. laczka.olivier@gmail.com.Cawthron Institute, 98 Halifax Street East, Private Bag 2, Nelson 7010, New Zealand. Tim.Harwood@cawthron.org.nz.Institute for Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia. r.lewis@imb.uq.edu.au.Institute for Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia. h.siddhihalu@imb.uq.edu.au.Climate Change Cluster, University of Technology Sydney, P.O. Box 123 Broadway, Sydney, NSW 2007, Australia. shauna.murray@uts.edu.au.Climate Change Cluster, University of Technology Sydney, P.O. Box 123 Broadway, Sydney, NSW 2007, Australia. Martina.Doblin@uts.edu.au.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29301247

Citation

Larsson, Michaela E., et al. "Toxicology of Gambierdiscus Spp. (Dinophyceae) From Tropical and Temperate Australian Waters." Marine Drugs, vol. 16, no. 1, 2018.
Larsson ME, Laczka OF, Harwood DT, et al. Toxicology of Gambierdiscus spp. (Dinophyceae) from Tropical and Temperate Australian Waters. Mar Drugs. 2018;16(1).
Larsson, M. E., Laczka, O. F., Harwood, D. T., Lewis, R. J., Himaya, S. W. A., Murray, S. A., & Doblin, M. A. (2018). Toxicology of Gambierdiscus spp. (Dinophyceae) from Tropical and Temperate Australian Waters. Marine Drugs, 16(1). https://doi.org/10.3390/md16010007
Larsson ME, et al. Toxicology of Gambierdiscus Spp. (Dinophyceae) From Tropical and Temperate Australian Waters. Mar Drugs. 2018 Jan 1;16(1) PubMed PMID: 29301247.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Toxicology of Gambierdiscus spp. (Dinophyceae) from Tropical and Temperate Australian Waters. AU - Larsson,Michaela E, AU - Laczka,Olivier F, AU - Harwood,D Tim, AU - Lewis,Richard J, AU - Himaya,S W A, AU - Murray,Shauna A, AU - Doblin,Martina A, Y1 - 2018/01/01/ PY - 2017/10/19/received PY - 2017/12/07/revised PY - 2017/12/15/accepted PY - 2018/1/6/entrez PY - 2018/1/6/pubmed PY - 2018/8/7/medline KW - Benthic Harmful Algal Bloom (BHAB) KW - Ciguatera Fish Poisoning (CFP) KW - Ciguatoxin (CTX) KW - Fluorescent Imaging Plate Reader (FLIPR) KW - Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) KW - Maitotoxin (MTX) JF - Marine drugs JO - Mar Drugs VL - 16 IS - 1 N2 - Ciguatera Fish Poisoning (CFP) is a human illness caused by the consumption of marine fish contaminated with ciguatoxins (CTX) and possibly maitotoxins (MTX), produced by species from the benthic dinoflagellate genus Gambierdiscus. Here, we describe the identity and toxicology of Gambierdiscus spp. isolated from the tropical and temperate waters of eastern Australia. Based on newly cultured strains, we found that four Gambierdiscus species were present at the tropical location, including G. carpenteri, G. lapillus and two others which were not genetically identical to other currently described species within the genus, and may represent new species. Only G. carpenteri was identified from the temperate location. Using LC-MS/MS analysis we did not find any characterized microalgal CTXs (P-CTX-3B, P-CTX-3C, P-CTX-4A and P-CTX-4B) or MTX-1; however, putative maitotoxin-3 (MTX-3) was detected in all species except for the temperate population of G. carpenteri. Using the Ca2+ influx SH-SY5Y cell Fluorescent Imaging Plate Reader (FLIPR) bioassay we found CTX-like activity in extracts of the unidentified Gambierdiscus strains and trace level activity in strains of G. lapillus. While no detectable CTX-like activity was observed in tropical or temperate strains of G. carpenteri, all species showed strong maitotoxin-like activity. This study, which represents the most comprehensive analyses of the toxicology of Gambierdiscus strains isolated from Australia to date, suggests that CFP in this region may be caused by currently undescribed ciguatoxins and maitotoxins. SN - 1660-3397 UR - https://www.unboundmedicine.com/medline/citation/29301247/Toxicology_of_Gambierdiscus_spp___Dinophyceae__from_Tropical_and_Temperate_Australian_Waters_ DB - PRIME DP - Unbound Medicine ER -