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In situ assessment of Karaj River genotoxic impact with the alkaline comet assay and micronucleus test, on feral brown trout (Salmo trutta fario).
Environ Toxicol Pharmacol. 2018 Mar; 58:59-69.ET

Abstract

The in situ evaluation of the genotoxic impact of the Karaj River was performed using the comet and micronucleus (MN) assays in erythrocytes, liver, gill and kidney of indigenous brown trout, Salmo trutta fario from three different stations, including Varangerud, Asara and Purkan. The results showed that DNA damage significantly increased in sampled fish erythrocytes, liver and gill from low levels in the upstream river (Varangerud) via intermediate levels in downstream (Purkan) to high levels in the middle of the river (Asara), correlating with the river increasing pollution gradient. Gill was the most sensitive tissue followed by blood and liver. Kidney did not respond to the genotoxic gradient of the river. MN test (as a complementary assay) of liver cells of fish was a sensitive biomarker of genotoxic exposure. MN test in blood, gill and kidney did not reflect the genotoxic condition of the river.

Authors+Show Affiliations

Department of Environment and Energy, Science and Research Branch Islamic Azad University, 14515/775, Tehran, Iran. Electronic address: mehran.hariri@igp.uu.se.Department of Fisheries, Faculty of Natural Resources, University of Tehran, 31585-4314, Karaj, Iran.Department of Fisheries, Faculty of Natural Resources, University of Tehran, 31585-4314, Karaj, Iran.Department of Environment and Energy, Science and Research Branch Islamic Azad University, 14515/775, Tehran, Iran.UNITWIN/UNESCO/WiCoP, Departamento de Química Física, Universidad de Cádiz, Facultad de Ciencias del Mary Ambientales, Campus Universitario de Puerto Real, 11510 Puerto Real, Cádiz, Spain.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29304400

Citation

Hariri, Mehran, et al. "In Situ Assessment of Karaj River Genotoxic Impact With the Alkaline Comet Assay and Micronucleus Test, On Feral Brown Trout (Salmo Trutta Fario)." Environmental Toxicology and Pharmacology, vol. 58, 2018, pp. 59-69.
Hariri M, Mirvaghefi A, Farahmand H, et al. In situ assessment of Karaj River genotoxic impact with the alkaline comet assay and micronucleus test, on feral brown trout (Salmo trutta fario). Environ Toxicol Pharmacol. 2018;58:59-69.
Hariri, M., Mirvaghefi, A., Farahmand, H., Taghavi, L., & Shahabinia, A. R. (2018). In situ assessment of Karaj River genotoxic impact with the alkaline comet assay and micronucleus test, on feral brown trout (Salmo trutta fario). Environmental Toxicology and Pharmacology, 58, 59-69. https://doi.org/10.1016/j.etap.2017.12.024
Hariri M, et al. In Situ Assessment of Karaj River Genotoxic Impact With the Alkaline Comet Assay and Micronucleus Test, On Feral Brown Trout (Salmo Trutta Fario). Environ Toxicol Pharmacol. 2018;58:59-69. PubMed PMID: 29304400.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In situ assessment of Karaj River genotoxic impact with the alkaline comet assay and micronucleus test, on feral brown trout (Salmo trutta fario). AU - Hariri,Mehran, AU - Mirvaghefi,Alireza, AU - Farahmand,Hamid, AU - Taghavi,Lobat, AU - Shahabinia,Amir-Reza, Y1 - 2017/12/28/ PY - 2017/02/07/received PY - 2017/11/07/revised PY - 2017/12/28/accepted PY - 2018/1/6/pubmed PY - 2018/8/28/medline PY - 2018/1/6/entrez KW - Comet assay KW - Genotoxic impact KW - In situ KW - Karaj River KW - Micronucleus test KW - Salmo trutta fario SP - 59 EP - 69 JF - Environmental toxicology and pharmacology JO - Environ Toxicol Pharmacol VL - 58 N2 - The in situ evaluation of the genotoxic impact of the Karaj River was performed using the comet and micronucleus (MN) assays in erythrocytes, liver, gill and kidney of indigenous brown trout, Salmo trutta fario from three different stations, including Varangerud, Asara and Purkan. The results showed that DNA damage significantly increased in sampled fish erythrocytes, liver and gill from low levels in the upstream river (Varangerud) via intermediate levels in downstream (Purkan) to high levels in the middle of the river (Asara), correlating with the river increasing pollution gradient. Gill was the most sensitive tissue followed by blood and liver. Kidney did not respond to the genotoxic gradient of the river. MN test (as a complementary assay) of liver cells of fish was a sensitive biomarker of genotoxic exposure. MN test in blood, gill and kidney did not reflect the genotoxic condition of the river. SN - 1872-7077 UR - https://www.unboundmedicine.com/medline/citation/29304400/In_situ_assessment_of_Karaj_River_genotoxic_impact_with_the_alkaline_comet_assay_and_micronucleus_test_on_feral_brown_trout__Salmo_trutta_fario__ DB - PRIME DP - Unbound Medicine ER -