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Synthesis, characterization and evaluation of antimicrobial and cytotoxic activities of biogenic silver nanoparticles synthesized from Streptomyces xinghaiensis OF1 strain.
World J Microbiol Biotechnol. 2018 Jan 05; 34(2):23.WJ

Abstract

We report synthesis of silver nanoparticles (AgNPs) from Streptomyces xinghaiensis OF1 strain, which were characterised by UV-Vis and Fourier transform infrared spectroscopy, Zeta sizer, Nano tracking analyser, and Transmission electron microscopy. The antimicrobial activity of AgNPs alone, and in combination with antibiotics was evaluated against bacteria, namely Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis, and yeasts viz., Candida albicans and Malassezia furfur by using micro-dilution method. The minimum inhibitory concentration (MIC) and minimum biocidal concentration of AgNPs against bacterial and yeast strains were determined. Synergistic effect of AgNPs in combination with antibacterial and antifungal antibiotics was determined by FIC index. In addition, MTT assay was performed to study cytotoxicity of AgNPs alone and in combination with antibiotics against mouse fibroblasts and HeLa cell line. Biogenic AgNPs were stable, spherical, small, polydispersed and capped with organic compounds. The variable antimicrobial activity of AgNPs was observed against tested bacteria and yeasts. The lowest MIC (16 µg ml-1) of AgNPs was found against P. aeruginosa, followed by C. albicans and M. furfur (both 32 µg ml-1), B. subtilis and E. coli (both 64 µg ml-1), and then S. aureus and Klebsiella pneumoniae (256 µg ml-1). The high synergistic effect of antibiotics in combination with AgNPs against tested strains was found. The in vitro cytotoxicity of AgNPs against mouse fibroblasts and cancer HeLa cell lines revealed a dose dependent potential. The IC50 value of AgNPs was found in concentrations of 4 and 3.8 µg ml-1, respectively. Combination of AgNPs and antibiotics significantly decreased concentrations of both antimicrobials used and retained their high antibacterial and antifungal activity. The synthesis of AgNPs using S. xinghaiensis OF1 strain is an eco-friendly, cheap and nontoxic method. The antimicrobial activity of AgNPs could result from their small size. Remarkable synergistic effect of antibiotics and AgNPs offer their valuable potential in nanomedicine for clinical application as a combined therapy in the future.

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Authors+Show Affiliations

Wypij M
Department of Microbiology, Nicolaus Copernicus University, Lwowska 1, 87 100, Toruń, Poland.
Czarnecka J
Department of Biochemistry, Nicolaus Copernicus University, Lwowska 1, 87 100, Toruń, Poland.
Świecimska M
Department of Microbiology, Nicolaus Copernicus University, Lwowska 1, 87 100, Toruń, Poland.
Dahm H
Department of Microbiology, Nicolaus Copernicus University, Lwowska 1, 87 100, Toruń, Poland.
Rai M
Nanobiotechnology Lab, Department of Biotechnology, SGB Amravati University, Amravati, Maharashtra, 444602, India.
Golinska P
Department of Microbiology, Nicolaus Copernicus University, Lwowska 1, 87 100, Toruń, Poland. golinska@umk.pl.

MeSH

AnimalsAnti-Bacterial AgentsAnti-Infective AgentsAntifungal AgentsBacillus subtilisCandida albicansDrug SynergismEscherichia coliFibroblastsHeLa CellsHumansIndiaKlebsiella pneumoniaeMetal NanoparticlesMiceMicrobial Sensitivity TestsMicroscopy, Electron, TransmissionPseudomonas aeruginosaSilverSpectroscopy, Fourier Transform InfraredStaphylococcus aureusStreptomyces

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29305718

Citation

Wypij, Magdalena, et al. "Synthesis, Characterization and Evaluation of Antimicrobial and Cytotoxic Activities of Biogenic Silver Nanoparticles Synthesized From Streptomyces Xinghaiensis OF1 Strain." World Journal of Microbiology & Biotechnology, vol. 34, no. 2, 2018, p. 23.
Wypij M, Czarnecka J, Świecimska M, et al. Synthesis, characterization and evaluation of antimicrobial and cytotoxic activities of biogenic silver nanoparticles synthesized from Streptomyces xinghaiensis OF1 strain. World J Microbiol Biotechnol. 2018;34(2):23.
Wypij, M., Czarnecka, J., Świecimska, M., Dahm, H., Rai, M., & Golinska, P. (2018). Synthesis, characterization and evaluation of antimicrobial and cytotoxic activities of biogenic silver nanoparticles synthesized from Streptomyces xinghaiensis OF1 strain. World Journal of Microbiology & Biotechnology, 34(2), 23. https://doi.org/10.1007/s11274-017-2406-3
Wypij M, et al. Synthesis, Characterization and Evaluation of Antimicrobial and Cytotoxic Activities of Biogenic Silver Nanoparticles Synthesized From Streptomyces Xinghaiensis OF1 Strain. World J Microbiol Biotechnol. 2018 Jan 5;34(2):23. PubMed PMID: 29305718.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synthesis, characterization and evaluation of antimicrobial and cytotoxic activities of biogenic silver nanoparticles synthesized from Streptomyces xinghaiensis OF1 strain. AU - Wypij,Magdalena, AU - Czarnecka,Joanna, AU - Świecimska,Magdalena, AU - Dahm,Hanna, AU - Rai,Mahendra, AU - Golinska,Patrycja, Y1 - 2018/01/05/ PY - 2017/06/21/received PY - 2017/12/30/accepted PY - 2018/1/7/entrez PY - 2018/1/7/pubmed PY - 2018/7/26/medline KW - Antibacterial activity KW - Antifungal activity KW - Antimicrobials KW - Biogenic silver nanoparticles KW - Cytotoxicity KW - Streptomycetes KW - Synergism SP - 23 EP - 23 JF - World journal of microbiology & biotechnology JO - World J Microbiol Biotechnol VL - 34 IS - 2 N2 - We report synthesis of silver nanoparticles (AgNPs) from Streptomyces xinghaiensis OF1 strain, which were characterised by UV-Vis and Fourier transform infrared spectroscopy, Zeta sizer, Nano tracking analyser, and Transmission electron microscopy. The antimicrobial activity of AgNPs alone, and in combination with antibiotics was evaluated against bacteria, namely Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis, and yeasts viz., Candida albicans and Malassezia furfur by using micro-dilution method. The minimum inhibitory concentration (MIC) and minimum biocidal concentration of AgNPs against bacterial and yeast strains were determined. Synergistic effect of AgNPs in combination with antibacterial and antifungal antibiotics was determined by FIC index. In addition, MTT assay was performed to study cytotoxicity of AgNPs alone and in combination with antibiotics against mouse fibroblasts and HeLa cell line. Biogenic AgNPs were stable, spherical, small, polydispersed and capped with organic compounds. The variable antimicrobial activity of AgNPs was observed against tested bacteria and yeasts. The lowest MIC (16 µg ml-1) of AgNPs was found against P. aeruginosa, followed by C. albicans and M. furfur (both 32 µg ml-1), B. subtilis and E. coli (both 64 µg ml-1), and then S. aureus and Klebsiella pneumoniae (256 µg ml-1). The high synergistic effect of antibiotics in combination with AgNPs against tested strains was found. The in vitro cytotoxicity of AgNPs against mouse fibroblasts and cancer HeLa cell lines revealed a dose dependent potential. The IC50 value of AgNPs was found in concentrations of 4 and 3.8 µg ml-1, respectively. Combination of AgNPs and antibiotics significantly decreased concentrations of both antimicrobials used and retained their high antibacterial and antifungal activity. The synthesis of AgNPs using S. xinghaiensis OF1 strain is an eco-friendly, cheap and nontoxic method. The antimicrobial activity of AgNPs could result from their small size. Remarkable synergistic effect of antibiotics and AgNPs offer their valuable potential in nanomedicine for clinical application as a combined therapy in the future. SN - 1573-0972 UR - https://www.unboundmedicine.com/medline/citation/29305718/Synthesis_characterization_and_evaluation_of_antimicrobial_and_cytotoxic_activities_of_biogenic_silver_nanoparticles_synthesized_from_Streptomyces_xinghaiensis_OF1_strain_ DB - PRIME DP - Unbound Medicine ER -