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Momordica charantia polysaccharides ameliorate oxidative stress, inflammation, and apoptosis in ethanol-induced gastritis in mucosa through NF-kB signaling pathway inhibition.
Int J Biol Macromol. 2018 May; 111:193-199.IJ

Abstract

This study investigated the therapeutic role of polysaccharides from M. charantia and their mechanism of action against ethanol-induced gastric ulcers in rats. Their effects were determined through macroscopic evaluation of the gastric cavity (gastric ulcer index [GUI]), changes in PGE2, lipid peroxidation (malondialdehyde), antioxidant systems (catalase and reduced glutathione), inflammatory markers (tumor necrosis factor-α [TNF-α], interleukin-6 [IL-6], and myeloperoxidase [MPO]), apoptotic markers (caspase 3, Bax, and Bcl-2), nuclear factor-κB (NF-κB [p65]), and histopathological staining (H&E and PAS). Pretreatment with MCP (300mg/kg p.o.) attenuated the severity of ethanol-induced gastric mucosal damage, reductions in GUI, histopathologic aberrations, and neutrophil invasion, and PGE2 upregulation. These actions were similar to those of omeprazole, a reference anti-ulcer drug. MCP repressed gastric inflammation through the reduction of MPO, TNF-α, and IL-6, and prevented gastric oxidative stress through the inhibition of lipid peroxides with the concomitant enhancement of glutathione and catalase activity. Apoptotic markers indicated that MCP suppressed Bax and caspase-3 activity and enhanced the anti-apoptotic protein Bcl-2, which favored cell survival. MCP downregulated NF-κB and upregulated IκBα. Our study results suggested that the prophylactic administration of MCP reduced ethanol-induced gastric injury in rats through the suppression of gastric inflammation and oxidative stress, predominantly via NF-κB inhibition.

Authors+Show Affiliations

Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia. Electronic address: mraish@ksu.edu.sa.Department Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.Department Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.Department Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.Department Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.Department Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.Quality Assurance Unit, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29307809

Citation

Raish, Mohammad, et al. "Momordica Charantia Polysaccharides Ameliorate Oxidative Stress, Inflammation, and Apoptosis in Ethanol-induced Gastritis in Mucosa Through NF-kB Signaling Pathway Inhibition." International Journal of Biological Macromolecules, vol. 111, 2018, pp. 193-199.
Raish M, Ahmad A, Ansari MA, et al. Momordica charantia polysaccharides ameliorate oxidative stress, inflammation, and apoptosis in ethanol-induced gastritis in mucosa through NF-kB signaling pathway inhibition. Int J Biol Macromol. 2018;111:193-199.
Raish, M., Ahmad, A., Ansari, M. A., Alkharfy, K. M., Aljenoobi, F. I., Jan, B. L., Al-Mohizea, A. M., Khan, A., & Ali, N. (2018). Momordica charantia polysaccharides ameliorate oxidative stress, inflammation, and apoptosis in ethanol-induced gastritis in mucosa through NF-kB signaling pathway inhibition. International Journal of Biological Macromolecules, 111, 193-199. https://doi.org/10.1016/j.ijbiomac.2018.01.008
Raish M, et al. Momordica Charantia Polysaccharides Ameliorate Oxidative Stress, Inflammation, and Apoptosis in Ethanol-induced Gastritis in Mucosa Through NF-kB Signaling Pathway Inhibition. Int J Biol Macromol. 2018;111:193-199. PubMed PMID: 29307809.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Momordica charantia polysaccharides ameliorate oxidative stress, inflammation, and apoptosis in ethanol-induced gastritis in mucosa through NF-kB signaling pathway inhibition. AU - Raish,Mohammad, AU - Ahmad,Ajaz, AU - Ansari,Mushtaq Ahmad, AU - Alkharfy,Khalid M, AU - Aljenoobi,Fahad I, AU - Jan,Basit L, AU - Al-Mohizea,Abdullah M, AU - Khan,Altaf, AU - Ali,Naushad, Y1 - 2018/01/04/ PY - 2017/11/27/received PY - 2017/12/27/revised PY - 2018/01/02/accepted PY - 2018/1/9/pubmed PY - 2018/8/30/medline PY - 2018/1/9/entrez KW - Apoptosis KW - Gastric injury KW - Momordica charantia polysaccharide KW - Oxidative stress SP - 193 EP - 199 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 111 N2 - This study investigated the therapeutic role of polysaccharides from M. charantia and their mechanism of action against ethanol-induced gastric ulcers in rats. Their effects were determined through macroscopic evaluation of the gastric cavity (gastric ulcer index [GUI]), changes in PGE2, lipid peroxidation (malondialdehyde), antioxidant systems (catalase and reduced glutathione), inflammatory markers (tumor necrosis factor-α [TNF-α], interleukin-6 [IL-6], and myeloperoxidase [MPO]), apoptotic markers (caspase 3, Bax, and Bcl-2), nuclear factor-κB (NF-κB [p65]), and histopathological staining (H&E and PAS). Pretreatment with MCP (300mg/kg p.o.) attenuated the severity of ethanol-induced gastric mucosal damage, reductions in GUI, histopathologic aberrations, and neutrophil invasion, and PGE2 upregulation. These actions were similar to those of omeprazole, a reference anti-ulcer drug. MCP repressed gastric inflammation through the reduction of MPO, TNF-α, and IL-6, and prevented gastric oxidative stress through the inhibition of lipid peroxides with the concomitant enhancement of glutathione and catalase activity. Apoptotic markers indicated that MCP suppressed Bax and caspase-3 activity and enhanced the anti-apoptotic protein Bcl-2, which favored cell survival. MCP downregulated NF-κB and upregulated IκBα. Our study results suggested that the prophylactic administration of MCP reduced ethanol-induced gastric injury in rats through the suppression of gastric inflammation and oxidative stress, predominantly via NF-κB inhibition. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/29307809/Momordica_charantia_polysaccharides_ameliorate_oxidative_stress_inflammation_and_apoptosis_in_ethanol_induced_gastritis_in_mucosa_through_NF_kB_signaling_pathway_inhibition_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(17)34711-6 DB - PRIME DP - Unbound Medicine ER -