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Effect of sevoflurane on the ATPase activity of hippocampal neurons in a rat model of cerebral ischemia-reperfusion injury via the cAMP-PKA signaling pathway.
Kaohsiung J Med Sci. 2018 Jan; 34(1):22-33.KJ

Abstract

We aim to investigate the effects of sevoflurane on the ATPase activity of the hippocampal neurons in rats with cerebral ischemia-reperfusion injury (IRI) via the cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) signaling pathway. Sixty rats were assigned into the normal, model and sevoflurane groups (n = 20, the latter two groups were established as focal cerebral IRI models). The ATPase activity was detected using an ultramicro Na (+)-K (+)-ATP enzyme kit. Immunohistochemical staining was used to detect the positive protein expression of cAMP and PKA. The hippocampal neurons were assigned to the normal, IRI, IRI + sevoflurane, IRI + forskolin, IRI + H89 and IRI + sevoflurane + H89 groups. qRT-PCR and Western blotting were performed for the expressions of cAMP, PKA, cAMP-responsive element-binding protein (CREB) and brain derived neurotrophic factor (BDNF). The normal and sevoflurane groups exhibited a greater positive protein expression of cAMP and PKA than the model group. Compared with the normal group, the expressions of cAMP, PKA, CREB and BDNF all reduced in the IRI, model and IRI + H89 groups. The sevoflurane group showed higher cAMP, PKA, CREB and BDNF expressions than the model group. Compared with the IRI group, ATPase activity and expressions of cAMP, PKA, CREB and BDNF all increased in the normal, IRI + sevoflurane and IRI + forskolin groups but decreased in the IRI + H89 group. It suggests that sevoflurane could enhance ATPase activity in hippocampal neurons of cerebral IRI rats through activating cAMP-PKA signaling pathway.

Authors+Show Affiliations

Department of Anesthesia, The Affiliated Hospital of North China University of Science and Technology, Tangshan, PR China.Department of Urology Surgery, The Affiliated Hospital of North China University of Science and Technology, Tangshan, PR China.Department of Anesthesia, The Affiliated Hospital of North China University of Science and Technology, Tangshan, PR China.Department of Anesthesia, The Affiliated Hospital of North China University of Science and Technology, Tangshan, PR China.Department of Critical Care Medicine, The Affiliated Hospital of North China University of Science and Technology, Tangshan, PR China.Department of Respiratory Medicine, The Affiliated Hospital of North China University of Science and Technology, Tangshan, PR China.Department of Critical Care Medicine, The Affiliated Hospital of North China University of Science and Technology, Tangshan, PR China.Department of Anesthesia, The Affiliated Hospital of North China University of Science and Technology, Tangshan, PR China. Electronic address: shubozsb17@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29310813

Citation

Liu, Tie-Jun, et al. "Effect of Sevoflurane On the ATPase Activity of Hippocampal Neurons in a Rat Model of Cerebral Ischemia-reperfusion Injury Via the cAMP-PKA Signaling Pathway." The Kaohsiung Journal of Medical Sciences, vol. 34, no. 1, 2018, pp. 22-33.
Liu TJ, Zhang JC, Gao XZ, et al. Effect of sevoflurane on the ATPase activity of hippocampal neurons in a rat model of cerebral ischemia-reperfusion injury via the cAMP-PKA signaling pathway. Kaohsiung J Med Sci. 2018;34(1):22-33.
Liu, T. J., Zhang, J. C., Gao, X. Z., Tan, Z. B., Wang, J. J., Zhang, P. P., Cheng, A. B., & Zhang, S. B. (2018). Effect of sevoflurane on the ATPase activity of hippocampal neurons in a rat model of cerebral ischemia-reperfusion injury via the cAMP-PKA signaling pathway. The Kaohsiung Journal of Medical Sciences, 34(1), 22-33. https://doi.org/10.1016/j.kjms.2017.09.004
Liu TJ, et al. Effect of Sevoflurane On the ATPase Activity of Hippocampal Neurons in a Rat Model of Cerebral Ischemia-reperfusion Injury Via the cAMP-PKA Signaling Pathway. Kaohsiung J Med Sci. 2018;34(1):22-33. PubMed PMID: 29310813.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of sevoflurane on the ATPase activity of hippocampal neurons in a rat model of cerebral ischemia-reperfusion injury via the cAMP-PKA signaling pathway. AU - Liu,Tie-Jun, AU - Zhang,Jin-Cun, AU - Gao,Xiao-Zeng, AU - Tan,Zhi-Bin, AU - Wang,Jian-Jun, AU - Zhang,Pan-Pan, AU - Cheng,Ai-Bin, AU - Zhang,Shu-Bo, Y1 - 2017/11/14/ PY - 2017/04/19/received PY - 2017/09/05/revised PY - 2017/09/13/accepted PY - 2018/1/10/entrez PY - 2018/1/10/pubmed PY - 2018/12/26/medline KW - ATPase activity KW - Cerebral ischemia-reperfusion injury KW - Hippocampal neuron KW - Sevoflurane KW - cAMP-PKA signaling pathway SP - 22 EP - 33 JF - The Kaohsiung journal of medical sciences JO - Kaohsiung J Med Sci VL - 34 IS - 1 N2 - We aim to investigate the effects of sevoflurane on the ATPase activity of the hippocampal neurons in rats with cerebral ischemia-reperfusion injury (IRI) via the cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) signaling pathway. Sixty rats were assigned into the normal, model and sevoflurane groups (n = 20, the latter two groups were established as focal cerebral IRI models). The ATPase activity was detected using an ultramicro Na (+)-K (+)-ATP enzyme kit. Immunohistochemical staining was used to detect the positive protein expression of cAMP and PKA. The hippocampal neurons were assigned to the normal, IRI, IRI + sevoflurane, IRI + forskolin, IRI + H89 and IRI + sevoflurane + H89 groups. qRT-PCR and Western blotting were performed for the expressions of cAMP, PKA, cAMP-responsive element-binding protein (CREB) and brain derived neurotrophic factor (BDNF). The normal and sevoflurane groups exhibited a greater positive protein expression of cAMP and PKA than the model group. Compared with the normal group, the expressions of cAMP, PKA, CREB and BDNF all reduced in the IRI, model and IRI + H89 groups. The sevoflurane group showed higher cAMP, PKA, CREB and BDNF expressions than the model group. Compared with the IRI group, ATPase activity and expressions of cAMP, PKA, CREB and BDNF all increased in the normal, IRI + sevoflurane and IRI + forskolin groups but decreased in the IRI + H89 group. It suggests that sevoflurane could enhance ATPase activity in hippocampal neurons of cerebral IRI rats through activating cAMP-PKA signaling pathway. SN - 2410-8650 UR - https://www.unboundmedicine.com/medline/citation/29310813/Effect_of_sevoflurane_on_the_ATPase_activity_of_hippocampal_neurons_in_a_rat_model_of_cerebral_ischemia_reperfusion_injury_via_the_cAMP_PKA_signaling_pathway_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1607-551X(17)30280-2 DB - PRIME DP - Unbound Medicine ER -