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Identification and Map-Based Cloning of the Light-Induced Lesion Mimic Mutant 1 (LIL1) Gene in Rice.
Front Plant Sci. 2017; 8:2122.FP

Abstract

The hypersensitive response (HR) is a mechanism by which plants prevent the spread of pathogen. Despite extensive study, the molecular mechanisms underlying HR remain poorly understood. Lesion mimic mutants (LMMs), such as LIL1 that was identified in an ethylmethane sulfonate mutagenized population of Indica rice (Oryza sativa L. ssp. Indica) 93-11, can be used to study the HR. Under natural field conditions, the leaves of LIL1 mutant plants exhibited light-induced, small, rust-red lesions that first appeared at the leaf tips and subsequently expanded throughout the entire leaf blade to the leaf sheath. Histochemical staining indicated that LIL1 lesions displayed an abnormal accumulation of reactive oxygen species (ROS) and resulted from programmed cell death (PCD). The LIL1 mutants also displayed increased expression of defense-related genes and enhanced resistance to rice blast fungus (Magnaporthe grisea). Genetic analysis showed that mutation of LIL1 created a semi-dominant allele. Using 1,758 individuals in the F2 population, LIL1 was mapped in a 222.3 kb region on the long arm of chromosome 7. That contains 12 predicted open reading frames (ORFs). Sequence analysis of these 12 candidate genes revealed a G to A base substitution in the fourth exon of LOC_Os07g30510, a putative cysteine-rich receptor-like kinase (CRK), which led to an amino acid change (Val 429 to Ile) in the LIL1 protein. Comparison of the transcript accumulation of the 12 candidate genes between LIL1 and 93-11 revealed that LOC_Os07g30510 was up-regulated significantly in LIL1. Overexpression of the LOC_Os07g30510 gene from LIL1 induced a LIL1-like lesion phenotype in Nipponbare. Thus, LIL1 is a novel LMM in rice that will facilitate the further study of the molecular mechanisms of HR and the rice blast resistance.

Authors+Show Affiliations

Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, China. Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop, Changsha, China.Agricultural College, Hunan Agricultural University, Changsha, China.Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, China.Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, China.Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop, Changsha, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29312386

Citation

Zhou, Qian, et al. "Identification and Map-Based Cloning of the Light-Induced Lesion Mimic Mutant 1 (LIL1) Gene in Rice." Frontiers in Plant Science, vol. 8, 2017, p. 2122.
Zhou Q, Zhang Z, Liu T, et al. Identification and Map-Based Cloning of the Light-Induced Lesion Mimic Mutant 1 (LIL1) Gene in Rice. Front Plant Sci. 2017;8:2122.
Zhou, Q., Zhang, Z., Liu, T., Gao, B., & Xiong, X. (2017). Identification and Map-Based Cloning of the Light-Induced Lesion Mimic Mutant 1 (LIL1) Gene in Rice. Frontiers in Plant Science, 8, 2122. https://doi.org/10.3389/fpls.2017.02122
Zhou Q, et al. Identification and Map-Based Cloning of the Light-Induced Lesion Mimic Mutant 1 (LIL1) Gene in Rice. Front Plant Sci. 2017;8:2122. PubMed PMID: 29312386.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and Map-Based Cloning of the Light-Induced Lesion Mimic Mutant 1 (LIL1) Gene in Rice. AU - Zhou,Qian, AU - Zhang,Zhifei, AU - Liu,Tiantian, AU - Gao,Bida, AU - Xiong,Xingyao, Y1 - 2017/12/19/ PY - 2016/12/29/received PY - 2017/11/29/accepted PY - 2018/1/10/entrez PY - 2018/1/10/pubmed PY - 2018/1/10/medline KW - hypersensitive response (HR) KW - lesion mimic mutant (LMM) KW - map-based cloning KW - rice (Oryza sativa L.) KW - rice blast resistance SP - 2122 EP - 2122 JF - Frontiers in plant science JO - Front Plant Sci VL - 8 N2 - The hypersensitive response (HR) is a mechanism by which plants prevent the spread of pathogen. Despite extensive study, the molecular mechanisms underlying HR remain poorly understood. Lesion mimic mutants (LMMs), such as LIL1 that was identified in an ethylmethane sulfonate mutagenized population of Indica rice (Oryza sativa L. ssp. Indica) 93-11, can be used to study the HR. Under natural field conditions, the leaves of LIL1 mutant plants exhibited light-induced, small, rust-red lesions that first appeared at the leaf tips and subsequently expanded throughout the entire leaf blade to the leaf sheath. Histochemical staining indicated that LIL1 lesions displayed an abnormal accumulation of reactive oxygen species (ROS) and resulted from programmed cell death (PCD). The LIL1 mutants also displayed increased expression of defense-related genes and enhanced resistance to rice blast fungus (Magnaporthe grisea). Genetic analysis showed that mutation of LIL1 created a semi-dominant allele. Using 1,758 individuals in the F2 population, LIL1 was mapped in a 222.3 kb region on the long arm of chromosome 7. That contains 12 predicted open reading frames (ORFs). Sequence analysis of these 12 candidate genes revealed a G to A base substitution in the fourth exon of LOC_Os07g30510, a putative cysteine-rich receptor-like kinase (CRK), which led to an amino acid change (Val 429 to Ile) in the LIL1 protein. Comparison of the transcript accumulation of the 12 candidate genes between LIL1 and 93-11 revealed that LOC_Os07g30510 was up-regulated significantly in LIL1. Overexpression of the LOC_Os07g30510 gene from LIL1 induced a LIL1-like lesion phenotype in Nipponbare. Thus, LIL1 is a novel LMM in rice that will facilitate the further study of the molecular mechanisms of HR and the rice blast resistance. SN - 1664-462X UR - https://www.unboundmedicine.com/medline/citation/29312386/Identification_and_Map_Based_Cloning_of_the_Light_Induced_Lesion_Mimic_Mutant_1__LIL1__Gene_in_Rice_ L2 - https://doi.org/10.3389/fpls.2017.02122 DB - PRIME DP - Unbound Medicine ER -
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