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Molecular Epidemiology of an Orientia tsutsugamushi Gene Encoding a 56-kDa Type-Specific Antigen in Chiggers, Small Mammals, and Patients from the Southwest Region of Korea.
Am J Trop Med Hyg. 2018 02; 98(2):616-624.AJ

Abstract

A phylogenetic analysis of Orientia tsutsugamushi was performed to elucidate its antigenic diversity in chiggers, small mammals, and patients. Between September 2014 and December 2016, a total of 3,816 chiggers were identified within nine species of four genera in the southwest region of Korea: Leptotrombidium scutellare (49.9%; 1,907/3,816), Leptotrombidium orientale (21.1%; 804/3,816), Leptotrombidium pallidum (12.4%; 474/3,816), Euchoengastia koreaensis (7.2%; 273/3,816), Leptotrombidium palpale (6.7%; 256/3,816), Neotrombicular gardellai (1.3%; 50/3,816), Leptotrombidium zetum (0.8%; 32/3,816), Walchia fragilis (0.5%; 18/3,816), and Neotrombicular japonica (> 0.1%; 2/3,816). Twelve chiggers (11 L. scutellare and one L. palpale) tested positive for O. tsutsugamushi by polymerase chain reaction and, except for 1 chigger (KY266830), were part of the Boryong strain cluster. Of the 413 small mammals that were analyzed for O. tsutsugamushi, Apodemus agrarius was the most common rodent species (89.5%; 370/413), followed by Crocidura lasiura (6.8%; 28/413) and Myodes regulus (3.6%; 15/413). The sequence identity of an O. tsutsugamushi sample obtained from the A. agrarius sample population belonged to the Saitama strain cluster. Furthermore, a phylogenetic analysis in 125 patients revealed four clusters (Boryong cluster: 82.4% [103/125], Karp: 13.6% [17/125], Kawasaki: 3.2% [4/125], and Saitama: 0.8% [1/125]). This study clarified the phylogenetic relationship for O. tsutsugamushi in chiggers, small mammals, and patients. The Boryong strain was the most common strain in chiggers and patients. In addition, various strains were identified, except for the Boryong strain, in the southwest region of Korea. Overall, the data presented here will be helpful for the establishment of prevention strategies for scrub typhus.

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Authors+Show Affiliations

Park JW
Division of Infectious disease investigation, Health and Environment Research Institute of Gwangju City, Gwangju, Korea.
Kim SH
Division of Infectious disease investigation, Health and Environment Research Institute of Gwangju City, Gwangju, Korea.
Park DW
Division of Infectious disease investigation, Health and Environment Research Institute of Gwangju City, Gwangju, Korea.
Jung SH
Division of Infectious disease investigation, Health and Environment Research Institute of Gwangju City, Gwangju, Korea.
Park HJ
Division of Infectious disease investigation, Health and Environment Research Institute of Gwangju City, Gwangju, Korea.
Seo MH
Division of Infectious disease investigation, Health and Environment Research Institute of Gwangju City, Gwangju, Korea.
Song HJ
Clinical Pathology, Gwangju Health University, Gwangju, Korea.
Lee JY
Clinical Pathology, Gwangju Health University, Gwangju, Korea.
Kim DM
Departments of Internal Medicine, School of Medicine, Chosun University, Gwangju, Korea.
Kim CM
Premedical Science, College of Medicine, Chosun University, Gwangju, Republic of Korea.
Gill BC
Division of Zoonoses, Korea Centers for Disease Control and Prevention, Cheongju, Korea.
Jeong HJ
Division of Zoonoses, Korea Centers for Disease Control and Prevention, Cheongju, Korea.
Lee JM
Division of Zoonoses, Korea Centers for Disease Control and Prevention, Cheongju, Korea.
Ha DR
Division of Infectious disease investigation, Health and Environment Research Institute of Gwangju City, Gwangju, Korea.
Kim ES
Division of Infectious disease investigation, Health and Environment Research Institute of Gwangju City, Gwangju, Korea.
Chung JK
Division of Infectious disease investigation, Health and Environment Research Institute of Gwangju City, Gwangju, Korea.

MeSH

AnimalsHumansMite InfestationsMolecular EpidemiologyOrientia tsutsugamushiPolymerase Chain ReactionRepublic of KoreaRodent DiseasesRodentiaTrombiculidae

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29313468

Citation

Park, Jung Wook, et al. "Molecular Epidemiology of an Orientia Tsutsugamushi Gene Encoding a 56-kDa Type-Specific Antigen in Chiggers, Small Mammals, and Patients From the Southwest Region of Korea." The American Journal of Tropical Medicine and Hygiene, vol. 98, no. 2, 2018, pp. 616-624.
Park JW, Kim SH, Park DW, et al. Molecular Epidemiology of an Orientia tsutsugamushi Gene Encoding a 56-kDa Type-Specific Antigen in Chiggers, Small Mammals, and Patients from the Southwest Region of Korea. Am J Trop Med Hyg. 2018;98(2):616-624.
Park, J. W., Kim, S. H., Park, D. W., Jung, S. H., Park, H. J., Seo, M. H., Song, H. J., Lee, J. Y., Kim, D. M., Kim, C. M., Gill, B. C., Jeong, H. J., Lee, J. M., Ha, D. R., Kim, E. S., & Chung, J. K. (2018). Molecular Epidemiology of an Orientia tsutsugamushi Gene Encoding a 56-kDa Type-Specific Antigen in Chiggers, Small Mammals, and Patients from the Southwest Region of Korea. The American Journal of Tropical Medicine and Hygiene, 98(2), 616-624. https://doi.org/10.4269/ajtmh.17-0070
Park JW, et al. Molecular Epidemiology of an Orientia Tsutsugamushi Gene Encoding a 56-kDa Type-Specific Antigen in Chiggers, Small Mammals, and Patients From the Southwest Region of Korea. Am J Trop Med Hyg. 2018;98(2):616-624. PubMed PMID: 29313468.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular Epidemiology of an Orientia tsutsugamushi Gene Encoding a 56-kDa Type-Specific Antigen in Chiggers, Small Mammals, and Patients from the Southwest Region of Korea. AU - Park,Jung Wook, AU - Kim,Sun Hee, AU - Park,Duck Woong, AU - Jung,So Hyang, AU - Park,Hye Jung, AU - Seo,Mi Hee, AU - Song,Hyeon Je, AU - Lee,Jung Yoon, AU - Kim,Dong Min, AU - Kim,Choon-Mee, AU - Gill,Byong Chul, AU - Jeong,Hang Jin, AU - Lee,Jeong Min, AU - Ha,Dong Ryong, AU - Kim,Eun Sun, AU - Chung,Jae Keun, Y1 - 2018/01/04/ PY - 2018/1/10/pubmed PY - 2018/12/12/medline PY - 2018/1/10/entrez SP - 616 EP - 624 JF - The American journal of tropical medicine and hygiene JO - Am J Trop Med Hyg VL - 98 IS - 2 N2 - A phylogenetic analysis of Orientia tsutsugamushi was performed to elucidate its antigenic diversity in chiggers, small mammals, and patients. Between September 2014 and December 2016, a total of 3,816 chiggers were identified within nine species of four genera in the southwest region of Korea: Leptotrombidium scutellare (49.9%; 1,907/3,816), Leptotrombidium orientale (21.1%; 804/3,816), Leptotrombidium pallidum (12.4%; 474/3,816), Euchoengastia koreaensis (7.2%; 273/3,816), Leptotrombidium palpale (6.7%; 256/3,816), Neotrombicular gardellai (1.3%; 50/3,816), Leptotrombidium zetum (0.8%; 32/3,816), Walchia fragilis (0.5%; 18/3,816), and Neotrombicular japonica (> 0.1%; 2/3,816). Twelve chiggers (11 L. scutellare and one L. palpale) tested positive for O. tsutsugamushi by polymerase chain reaction and, except for 1 chigger (KY266830), were part of the Boryong strain cluster. Of the 413 small mammals that were analyzed for O. tsutsugamushi, Apodemus agrarius was the most common rodent species (89.5%; 370/413), followed by Crocidura lasiura (6.8%; 28/413) and Myodes regulus (3.6%; 15/413). The sequence identity of an O. tsutsugamushi sample obtained from the A. agrarius sample population belonged to the Saitama strain cluster. Furthermore, a phylogenetic analysis in 125 patients revealed four clusters (Boryong cluster: 82.4% [103/125], Karp: 13.6% [17/125], Kawasaki: 3.2% [4/125], and Saitama: 0.8% [1/125]). This study clarified the phylogenetic relationship for O. tsutsugamushi in chiggers, small mammals, and patients. The Boryong strain was the most common strain in chiggers and patients. In addition, various strains were identified, except for the Boryong strain, in the southwest region of Korea. Overall, the data presented here will be helpful for the establishment of prevention strategies for scrub typhus. SN - 1476-1645 UR - https://www.unboundmedicine.com/medline/citation/29313468/Molecular_Epidemiology_of_an_Orientia_tsutsugamushi_Gene_Encoding_a_56_kDa_Type_Specific_Antigen_in_Chiggers_Small_Mammals_and_Patients_from_the_Southwest_Region_of_Korea_ DB - PRIME DP - Unbound Medicine ER -