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Exogenous 24-Epibrassinolide Interacts with Light to Regulate Anthocyanin and Proanthocyanidin Biosynthesis in Cabernet Sauvignon (Vitis vinifera L.).
Molecules. 2018 Jan 09; 23(1)M

Abstract

Anthocyanins and proanthocyanidins (PAs) are crucial factors that affect the quality of grapes and the making of wine, which were stimulated by various stimuli and environment factors (sugar, hormones, light, and temperature). The aim of the study was to investigate the influence of exogenous 24-Epibrassinolide (EBR) and light on the mechanism of anthocyanins and PAs accumulation in grape berries. Grape clusters were sprayed with EBR (0.4 mg/L) under light and darkness conditions (EBR + L, EBR + D), or sprayed with deionized water under light and darkness conditions as controls (L, D), at the onset of veraison. A large amount of anthocyanins accumulated in the grape skins and was measured under EBR + L and L treatments, whereas EBR + D and D treatments severely suppressed anthocyanin accumulation. This indicated that EBR treatment could produce overlay effects under light, in comparison to that in dark. Real-time quantitative PCR analysis indicated that EBR application up-regulated the expression of genes (VvCHI1, VvCHS2, VvCHS3, VvDFR, VvLDOX, VvMYBA1) under light conditions. Under darkness conditions, only early biosynthetic genes of anthocyanin biosynthesis responded to EBR. Furthermore, we also analyzed the expression levels of the BR-regulated transcription factor VvBZR1 (Brassinazole-resistant 1) and light-regulated transcription factor VvHY5 (Elongated hypocotyl 5). Our results suggested that EBR and light had synergistic effects on the expression of genes in the anthocyanin biosynthesis pathway.

Authors+Show Affiliations

College of Enology, Northwest A&F University, Yangling 712100, China. zhouyali@nwafu.edu.cn.College of Enology, Northwest A&F University, Yangling 712100, China. yuanchl69@nwsuaf.edu.cn. Shaanxi Engineering Research Center for Viti-Viniculture, Yangling 712100, China. yuanchl69@nwsuaf.edu.cn.Chateau Changyu Rena Co., Ltd., Xianyang 712000, China. ruanshicheng@sina.cn.College of Enology, Northwest A&F University, Yangling 712100, China. zhangzhw60@nwsuaf.edu.cn. Shaanxi Engineering Research Center for Viti-Viniculture, Yangling 712100, China. zhangzhw60@nwsuaf.edu.cn.College of Enology, Northwest A&F University, Yangling 712100, China. mjfwine@nwafu.edu.cn. Shaanxi Engineering Research Center for Viti-Viniculture, Yangling 712100, China. mjfwine@nwafu.edu.cn.College of Enology, Northwest A&F University, Yangling 712100, China. xizhumei@nwsuaf.edu.cn. Shaanxi Engineering Research Center for Viti-Viniculture, Yangling 712100, China. xizhumei@nwsuaf.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29315208

Citation

Zhou, Yali, et al. "Exogenous 24-Epibrassinolide Interacts With Light to Regulate Anthocyanin and Proanthocyanidin Biosynthesis in Cabernet Sauvignon (Vitis Vinifera L.)." Molecules (Basel, Switzerland), vol. 23, no. 1, 2018.
Zhou Y, Yuan C, Ruan S, et al. Exogenous 24-Epibrassinolide Interacts with Light to Regulate Anthocyanin and Proanthocyanidin Biosynthesis in Cabernet Sauvignon (Vitis vinifera L.). Molecules. 2018;23(1).
Zhou, Y., Yuan, C., Ruan, S., Zhang, Z., Meng, J., & Xi, Z. (2018). Exogenous 24-Epibrassinolide Interacts with Light to Regulate Anthocyanin and Proanthocyanidin Biosynthesis in Cabernet Sauvignon (Vitis vinifera L.). Molecules (Basel, Switzerland), 23(1). https://doi.org/10.3390/molecules23010093
Zhou Y, et al. Exogenous 24-Epibrassinolide Interacts With Light to Regulate Anthocyanin and Proanthocyanidin Biosynthesis in Cabernet Sauvignon (Vitis Vinifera L.). Molecules. 2018 Jan 9;23(1) PubMed PMID: 29315208.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exogenous 24-Epibrassinolide Interacts with Light to Regulate Anthocyanin and Proanthocyanidin Biosynthesis in Cabernet Sauvignon (Vitis vinifera L.). AU - Zhou,Yali, AU - Yuan,Chunlong, AU - Ruan,Shicheng, AU - Zhang,Zhenwen, AU - Meng,Jiangfei, AU - Xi,Zhumei, Y1 - 2018/01/09/ PY - 2017/11/02/received PY - 2017/12/28/revised PY - 2017/12/29/accepted PY - 2018/1/10/entrez PY - 2018/1/10/pubmed PY - 2018/11/22/medline KW - 24-Epibrassinolide KW - VvBZR1 KW - VvHY5 KW - anthocyanin biosynthesis KW - grape JF - Molecules (Basel, Switzerland) JO - Molecules VL - 23 IS - 1 N2 - Anthocyanins and proanthocyanidins (PAs) are crucial factors that affect the quality of grapes and the making of wine, which were stimulated by various stimuli and environment factors (sugar, hormones, light, and temperature). The aim of the study was to investigate the influence of exogenous 24-Epibrassinolide (EBR) and light on the mechanism of anthocyanins and PAs accumulation in grape berries. Grape clusters were sprayed with EBR (0.4 mg/L) under light and darkness conditions (EBR + L, EBR + D), or sprayed with deionized water under light and darkness conditions as controls (L, D), at the onset of veraison. A large amount of anthocyanins accumulated in the grape skins and was measured under EBR + L and L treatments, whereas EBR + D and D treatments severely suppressed anthocyanin accumulation. This indicated that EBR treatment could produce overlay effects under light, in comparison to that in dark. Real-time quantitative PCR analysis indicated that EBR application up-regulated the expression of genes (VvCHI1, VvCHS2, VvCHS3, VvDFR, VvLDOX, VvMYBA1) under light conditions. Under darkness conditions, only early biosynthetic genes of anthocyanin biosynthesis responded to EBR. Furthermore, we also analyzed the expression levels of the BR-regulated transcription factor VvBZR1 (Brassinazole-resistant 1) and light-regulated transcription factor VvHY5 (Elongated hypocotyl 5). Our results suggested that EBR and light had synergistic effects on the expression of genes in the anthocyanin biosynthesis pathway. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/29315208/Exogenous_24_Epibrassinolide_Interacts_with_Light_to_Regulate_Anthocyanin_and_Proanthocyanidin_Biosynthesis_in_Cabernet_Sauvignon__Vitis_vinifera_L___ L2 - https://www.mdpi.com/resolver?pii=molecules23010093 DB - PRIME DP - Unbound Medicine ER -