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[Mechanism of action for oligomeric proanthocyaniclins in pava qnat-induced acute lung injury].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2017 Nov 20; 35(11):818-822.ZL

Abstract

Objective:

The present study was designed to evaluate the protective effects of oligomeric proanthocyanidins (OPC) in mice exposed to paraquat (PQ) , and to explore the molecular mechanism.

Methods:

Four experimental groups were designed.

CONTROL GROUP

10 BALB/c mice were intraperitoneally injected with normal saline) . PQ group: 10 BALB/c mice were intraperitoneally injected with PQ (100 mg/kg) . PQ+OPC group: 10 BALB/c mice were administered with OPC (100 mg/kg) for 1 h before PQ (100 mg/kg) expo-sure. OPC group: 10 BALB/c mice were intraperitoneally injected with OPC (100 mg/kg) . The peripheral blood samples or lung tissue samples were collected at the designed time points for measuring the levels of oxi-dative stress indicators, the related protein levels of nuclear factor-kappa B (NF-κB) pathway and nuclear fac-tor erythroid related factor-2 (Nrf2) pathway. Results: Compared with the control group, the level of reactive oxygen species (ROS) , the content of malondialdehyde (MDA) in the PQ group were significantly induced, and the activity of superoxide dismutase (SOD) in the PQ group was decreased in the peripheral blood. As com-pared with the PQ group, the level of ROS and the content of MDA in the PQ+OPC group were significantly re-duced, the activity SOD in the PQ+OPC group was increased in the peripheral blood; the level of ROS and the content of MDA were also reduced in lung tissues in the PQ+OPC group. Moreover, compared with the con-trol group, the phosphorylation of IκBα and the expression of NF-κB p65 were increased in lung tissues in the PQ group. The phosphorylation of IκBα and the expression of NF-κB p65 were decreased in lung tissues in the PQ+OPC group as compared with the PQ group. In addition, compared with the control group, the expressions of HO-1 and Nrf2 were increased in lung tissues in OPC group, and these were decreased in lung tissues in PQ groups. Furthermore, the expressions of HO-1 and Nrf2 were also increased in lung tissues in PQ+OPC as com-pared with the PQ group. Conclusion: OPC could alleviate PQ-induced systemic toxicity in mice by regulating oxidative stress via NF-κB and Nrf2 pathway.

Authors+Show Affiliations

Department of Emergency, Intensive Care Medicine Center, 302 Hospital of PLA, Beijing 100039, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

29316751

Citation

Liu, P, et al. "[Mechanism of Action for Oligomeric Proanthocyaniclins in Pava Qnat-induced Acute Lung Injury]." Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi = Zhonghua Laodong Weisheng Zhiyebing Zazhi = Chinese Journal of Industrial Hygiene and Occupational Diseases, vol. 35, no. 11, 2017, pp. 818-822.
Liu P, Zhou YS, Qin YL, et al. [Mechanism of action for oligomeric proanthocyaniclins in pava qnat-induced acute lung injury]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2017;35(11):818-822.
Liu, P., Zhou, Y. S., Qin, Y. L., Li, L., Liu, Y., Xu, B., Huang, K., Ji, C. C., Lin, F., Wang, Y. G., Li, K., Chen, S. H., Shao, L. F., & Mu, J. S. (2017). [Mechanism of action for oligomeric proanthocyaniclins in pava qnat-induced acute lung injury]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi = Zhonghua Laodong Weisheng Zhiyebing Zazhi = Chinese Journal of Industrial Hygiene and Occupational Diseases, 35(11), 818-822. https://doi.org/10.3760/cma.j.issn.1001-9391.2017.11.004
Liu P, et al. [Mechanism of Action for Oligomeric Proanthocyaniclins in Pava Qnat-induced Acute Lung Injury]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2017 Nov 20;35(11):818-822. PubMed PMID: 29316751.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Mechanism of action for oligomeric proanthocyaniclins in pava qnat-induced acute lung injury]. AU - Liu,P, AU - Zhou,Y S, AU - Qin,Y L, AU - Li,L, AU - Liu,Y, AU - Xu,B, AU - Huang,K, AU - Ji,C C, AU - Lin,F, AU - Wang,Y G, AU - Li,K, AU - Chen,S H, AU - Shao,L F, AU - Mu,J S, PY - 2018/1/11/entrez PY - 2018/1/11/pubmed PY - 2018/10/26/medline KW - Acute lung injury KW - Oligomeric proanthocyanidins KW - Oxidative stress KW - Paraquat SP - 818 EP - 822 JF - Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases JO - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi VL - 35 IS - 11 N2 - : Objective: The present study was designed to evaluate the protective effects of oligomeric proanthocyanidins (OPC) in mice exposed to paraquat (PQ) , and to explore the molecular mechanism. Methods: Four experimental groups were designed. CONTROL GROUP: 10 BALB/c mice were intraperitoneally injected with normal saline) . PQ group: 10 BALB/c mice were intraperitoneally injected with PQ (100 mg/kg) . PQ+OPC group: 10 BALB/c mice were administered with OPC (100 mg/kg) for 1 h before PQ (100 mg/kg) expo-sure. OPC group: 10 BALB/c mice were intraperitoneally injected with OPC (100 mg/kg) . The peripheral blood samples or lung tissue samples were collected at the designed time points for measuring the levels of oxi-dative stress indicators, the related protein levels of nuclear factor-kappa B (NF-κB) pathway and nuclear fac-tor erythroid related factor-2 (Nrf2) pathway. Results: Compared with the control group, the level of reactive oxygen species (ROS) , the content of malondialdehyde (MDA) in the PQ group were significantly induced, and the activity of superoxide dismutase (SOD) in the PQ group was decreased in the peripheral blood. As com-pared with the PQ group, the level of ROS and the content of MDA in the PQ+OPC group were significantly re-duced, the activity SOD in the PQ+OPC group was increased in the peripheral blood; the level of ROS and the content of MDA were also reduced in lung tissues in the PQ+OPC group. Moreover, compared with the con-trol group, the phosphorylation of IκBα and the expression of NF-κB p65 were increased in lung tissues in the PQ group. The phosphorylation of IκBα and the expression of NF-κB p65 were decreased in lung tissues in the PQ+OPC group as compared with the PQ group. In addition, compared with the control group, the expressions of HO-1 and Nrf2 were increased in lung tissues in OPC group, and these were decreased in lung tissues in PQ groups. Furthermore, the expressions of HO-1 and Nrf2 were also increased in lung tissues in PQ+OPC as com-pared with the PQ group. Conclusion: OPC could alleviate PQ-induced systemic toxicity in mice by regulating oxidative stress via NF-κB and Nrf2 pathway. SN - 1001-9391 UR - https://www.unboundmedicine.com/medline/citation/29316751/[Mechanism_of_action_for_oligomeric_proanthocyaniclins_in_pava_qnat_induced_acute_lung_injury]_ DB - PRIME DP - Unbound Medicine ER -