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Protective effects of Mentha piperita L. leaf essential oil against CCl4 induced hepatic oxidative damage and renal failure in rats.
Lipids Health Dis. 2018 Jan 09; 17(1):9.LH

Abstract

BACKGROUND

Mentha piperita L. is a flowering plant belonging to the Lamiaceae family. Mentha plants constitute one of the main valuable sources of essential oil used in foods and for medicinal purposes.

METHODS

The present study aimed to investigate the composition and in vitro antioxidant activity of Mentha piperita leaf essential oil (MpEO). A single dose of CCl4 was used to induce oxidative stress in rats, which was demonstrated by a significant rise of serum enzyme markers. MpEO was administrated for 7 consecutive days (5, 15, 40 mg/kg body weight) to Wistar rats prior to CCl4 treatment and the effects on serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and γ -glutamyl transpeptidase (γ-GT) levels, as well as the liver and kidney superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activity and thiobarbituric acid reactive substances (TBARS) levels were evaluated. In addition, histopathological examinations of livers and kidneys was performed.

RESULTS

The in vitro antioxidant activity of MpEO was lower than that of silymarin. Pretreatment of animals with MpEO at a dose of 5 mg/kg did not have a significant effect on ALT, AST, ALP, LDH, γGT, urea or creatinine levels in CCl4-induced stress. Whereas pretreatment with MpEO at doses of 15 and 40 mg/kg prior to CCl4, significantly reduced stress parameters (ALT, AST, ALP, LDH, γGT, urea and creatinine) compared to the CCl4-only group. Moreover, a significant reduction in hepatic and kidney lipid peroxidation (TBARS) and an increase in antioxidant enzymes SOD, CAT and GPx was also observed after treatment with MpEO (40 mg/kg) compared to CCl4-treated rats. Furthermore, pretreatment with MpEO at 40 mg/kg can also markedly ameliorate the histopathological hepatic and kidney lesions induced by administration of CCl4.

CONCLUSIONS

We could demonstrate with this study that MpEO protects liver and kidney from CCl4-induced oxidative stress and thus substantiate the beneficial effects attributed traditionally to this plant.

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Authors+Show Affiliations

Bellassoued K
Department of Life Sciences, Animal Ecophysiology Laboratory, Faculty of sciences, University of Sfax Tunisia, Road of Soukra Km 3.5, BP 1171, PC 3000, Sfax, Tunisia. khaledbra3@gmail.com.
Ben Hsouna A
Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax, PO Box 1177, Road Sidi Mansour km 6, 3018, Sfax, Tunisia.
Athmouni K
Department of life sciences, Laboratory of Biodiversity and Aquatic Ecosystems, Faculty of sciences, University of Sfax Tunisia, Road of Soukra Km 3.5, BP 1171, PC 3000, Sfax, Tunisia.
van Pelt J
Laboratory of Clinical Digestive Oncology, Department of Oncology, KU, Leuven, Belgium.
Makni Ayadi F
Biochemistry Laboratory, CHU Habib Bourguiba of Sfax, Faculty of Medicine, University of Sfax Tunisia, Road Menzel Chaker km 0.5, CP 3029 Sfax, Sfax, Tunisia.
Rebai T
Laboratory of Histology and Embryology, Faculty of Medicine of Sfax, University of Sfax Tunisia, Road Menzel Chaker km 0.5, CP 3029, Sfax, Tunisia.
Elfeki A
Department of Life Sciences, Animal Ecophysiology Laboratory, Faculty of sciences, University of Sfax Tunisia, Road of Soukra Km 3.5, BP 1171, PC 3000, Sfax, Tunisia.

MeSH

Alanine TransaminaseAlkaline PhosphataseAnimalsAnti-Inflammatory AgentsAntioxidantsAspartate AminotransferasesCarbon TetrachlorideCatalaseChemical and Drug Induced Liver InjuryDrug Administration ScheduleGlutathione PeroxidaseKidneyL-Lactate DehydrogenaseLiverMaleMentha piperitaOils, VolatileOxidative StressPlant ExtractsRatsRats, WistarRenal InsufficiencySuperoxide DismutaseThiobarbituric Acid Reactive Substancesgamma-Glutamyltransferase

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29316974

Citation

Bellassoued, Khaled, et al. "Protective Effects of Mentha Piperita L. Leaf Essential Oil Against CCl4 Induced Hepatic Oxidative Damage and Renal Failure in Rats." Lipids in Health and Disease, vol. 17, no. 1, 2018, p. 9.
Bellassoued K, Ben Hsouna A, Athmouni K, et al. Protective effects of Mentha piperita L. leaf essential oil against CCl4 induced hepatic oxidative damage and renal failure in rats. Lipids Health Dis. 2018;17(1):9.
Bellassoued, K., Ben Hsouna, A., Athmouni, K., van Pelt, J., Makni Ayadi, F., Rebai, T., & Elfeki, A. (2018). Protective effects of Mentha piperita L. leaf essential oil against CCl4 induced hepatic oxidative damage and renal failure in rats. Lipids in Health and Disease, 17(1), 9. https://doi.org/10.1186/s12944-017-0645-9
Bellassoued K, et al. Protective Effects of Mentha Piperita L. Leaf Essential Oil Against CCl4 Induced Hepatic Oxidative Damage and Renal Failure in Rats. Lipids Health Dis. 2018 Jan 9;17(1):9. PubMed PMID: 29316974.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of Mentha piperita L. leaf essential oil against CCl4 induced hepatic oxidative damage and renal failure in rats. AU - Bellassoued,Khaled, AU - Ben Hsouna,Anis, AU - Athmouni,Khaled, AU - van Pelt,Jos, AU - Makni Ayadi,Fatma, AU - Rebai,Tarek, AU - Elfeki,Abdelfattah, Y1 - 2018/01/09/ PY - 2017/09/29/received PY - 2017/12/17/accepted PY - 2018/1/11/entrez PY - 2018/1/11/pubmed PY - 2018/8/7/medline KW - Essential oil KW - Histopathological KW - Kidney KW - Liver KW - M. piperita KW - Oxidative stress SP - 9 EP - 9 JF - Lipids in health and disease JO - Lipids Health Dis VL - 17 IS - 1 N2 - BACKGROUND: Mentha piperita L. is a flowering plant belonging to the Lamiaceae family. Mentha plants constitute one of the main valuable sources of essential oil used in foods and for medicinal purposes. METHODS: The present study aimed to investigate the composition and in vitro antioxidant activity of Mentha piperita leaf essential oil (MpEO). A single dose of CCl4 was used to induce oxidative stress in rats, which was demonstrated by a significant rise of serum enzyme markers. MpEO was administrated for 7 consecutive days (5, 15, 40 mg/kg body weight) to Wistar rats prior to CCl4 treatment and the effects on serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and γ -glutamyl transpeptidase (γ-GT) levels, as well as the liver and kidney superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activity and thiobarbituric acid reactive substances (TBARS) levels were evaluated. In addition, histopathological examinations of livers and kidneys was performed. RESULTS: The in vitro antioxidant activity of MpEO was lower than that of silymarin. Pretreatment of animals with MpEO at a dose of 5 mg/kg did not have a significant effect on ALT, AST, ALP, LDH, γGT, urea or creatinine levels in CCl4-induced stress. Whereas pretreatment with MpEO at doses of 15 and 40 mg/kg prior to CCl4, significantly reduced stress parameters (ALT, AST, ALP, LDH, γGT, urea and creatinine) compared to the CCl4-only group. Moreover, a significant reduction in hepatic and kidney lipid peroxidation (TBARS) and an increase in antioxidant enzymes SOD, CAT and GPx was also observed after treatment with MpEO (40 mg/kg) compared to CCl4-treated rats. Furthermore, pretreatment with MpEO at 40 mg/kg can also markedly ameliorate the histopathological hepatic and kidney lesions induced by administration of CCl4. CONCLUSIONS: We could demonstrate with this study that MpEO protects liver and kidney from CCl4-induced oxidative stress and thus substantiate the beneficial effects attributed traditionally to this plant. SN - 1476-511X UR - https://www.unboundmedicine.com/medline/citation/29316974/Protective_effects_of_Mentha_piperita_L__leaf_essential_oil_against_CCl4_induced_hepatic_oxidative_damage_and_renal_failure_in_rats_ DB - PRIME DP - Unbound Medicine ER -