Tags

Type your tag names separated by a space and hit enter

Engineered polyketides: Synergy between protein and host level engineering.
Synth Syst Biotechnol. 2017 Sep; 2(3):147-166.SS

Abstract

Metabolic engineering efforts toward rewiring metabolism of cells to produce new compounds often require the utilization of non-native enzymatic machinery that is capable of producing a broad range of chemical functionalities. Polyketides encompass one of the largest classes of chemically diverse natural products. With thousands of known polyketides, modular polyketide synthases (PKSs) share a particularly attractive biosynthetic logic for generating chemical diversity. The engineering of modular PKSs could open access to the deliberate production of both existing and novel compounds. In this review, we discuss PKS engineering efforts applied at both the protein and cellular level for the generation of a diverse range of chemical structures, and we examine future applications of PKSs in the production of medicines, fuels and other industrially relevant chemicals.

Authors+Show Affiliations

Department of Energy Agile BioFoundry, Emeryville, CA, USA.Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.Joint BioEnergy Institute, Emeryville, CA 94608, USA. Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Comparative Biochemistry Graduate Group, University of California, Berkeley, Berkeley, CA 94720, USA.Joint BioEnergy Institute, Emeryville, CA 94608, USA. Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. QB3 Institute, University of California, Berkeley, Emeryville, CA 94608, USA. Department of Chemical & Biomolecular Engineering, Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA. Novo Nordisk Foundation Center for Biosustainability, Technical University Denmark, DK2970 Horsholm, Denmark.

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

29318196

Citation

Barajas, Jesus F., et al. "Engineered Polyketides: Synergy Between Protein and Host Level Engineering." Synthetic and Systems Biotechnology, vol. 2, no. 3, 2017, pp. 147-166.
Barajas JF, Blake-Hedges JM, Bailey CB, et al. Engineered polyketides: Synergy between protein and host level engineering. Synthetic and systems biotechnology. 2017;2(3):147-166.
Barajas, J. F., Blake-Hedges, J. M., Bailey, C. B., Curran, S., & Keasling, J. D. (2017). Engineered polyketides: Synergy between protein and host level engineering. Synthetic and Systems Biotechnology, 2(3), 147-166. https://doi.org/10.1016/j.synbio.2017.08.005
Barajas JF, et al. Engineered Polyketides: Synergy Between Protein and Host Level Engineering. Synthetic and systems biotechnology. 2017;2(3):147-166. PubMed PMID: 29318196.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Engineered polyketides: Synergy between protein and host level engineering. AU - Barajas,Jesus F, AU - Blake-Hedges,Jacquelyn M, AU - Bailey,Constance B, AU - Curran,Samuel, AU - Keasling,Jay D, Y1 - 2017/09/07/ PY - 2017/07/31/received PY - 2017/08/26/revised PY - 2017/08/26/accepted PY - 2018/1/11/entrez PY - 2018/1/11/pubmed PY - 2018/1/11/medline KW - ACP, Acyl carrier protein KW - AT, Acyltransferase KW - CoL, CoA-Ligase KW - Commodity chemical KW - DE, Dimerization element KW - DEBS, 6-deoxyerythronolide B synthase KW - DH, Dehydratase KW - ER, Enoylreductase KW - FAS, Fatty acid synthases KW - KR, Ketoreductase KW - KS, Ketosynthase KW - LM, Loading module KW - LTTR, LysR-type transcriptional regulator KW - Metabolic engineering KW - Natural products KW - PCC, Propionyl-CoA carboxylase KW - PDB, Precursor directed biosynthesis KW - PK, Polyketide KW - PKS, Polyketide synthase KW - Polyketide KW - Polyketide synthase KW - R, Reductase domain KW - SARP, Streptomyces antibiotic regulatory protein KW - SNAC, N-acetylcysteamine KW - Synthetic biology KW - TE, Thioesterase KW - TKL, Triketide lactone SP - 147 EP - 166 JF - Synthetic and systems biotechnology VL - 2 IS - 3 N2 - Metabolic engineering efforts toward rewiring metabolism of cells to produce new compounds often require the utilization of non-native enzymatic machinery that is capable of producing a broad range of chemical functionalities. Polyketides encompass one of the largest classes of chemically diverse natural products. With thousands of known polyketides, modular polyketide synthases (PKSs) share a particularly attractive biosynthetic logic for generating chemical diversity. The engineering of modular PKSs could open access to the deliberate production of both existing and novel compounds. In this review, we discuss PKS engineering efforts applied at both the protein and cellular level for the generation of a diverse range of chemical structures, and we examine future applications of PKSs in the production of medicines, fuels and other industrially relevant chemicals. SN - 2405-805X UR - https://www.unboundmedicine.com/medline/citation/29318196/Engineered_polyketides:_Synergy_between_protein_and_host_level_engineering_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S2405-805X(17)30085-6 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.