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[Effect of Moxibustion Preconditioning with Seed-sized Moxa Cones on Myocardial Infarction Size and Beclin 1 Expression in Myocardial Ischemia-reperfusion Injury Rats].
Zhen Ci Yan Jiu. 2017 Dec 25; 42(6):471-6.ZC

Abstract

OBJECTIVE

To observe the effect of moxibustion (Moxi) preconditioning with seed-sized moxa cones on myocardial ischemia-reperfusion injury (MI/RI) at different stages, and to analyze the correlation between this effect and the expression of autophagy related protein Beclin 1.

METHODS

This study contains two parts: 1) changes of myocardial pathological injury and percentages of myocardial infarcted area at different time-points after modeling and Moxi intervention, and 2) effect of Moxi on contents of serum cardiac troponin T(cTnT) and expression of myocardial Bcl-2, Bax and Beclin 1 proteins. In the first part, 42 SD rats were randomly divided into model group, 1 day (d) Moxi group, 2 d Moxi group, 3 d Moxi group,4 d Moxi group, 5 d Moxi group and 7 d Moxi group. The model of MI/RI was established by ligating the left anterior descending coronary artery (LAD) for 30 min and reperfusion for 240 min. The electrocardiogram (ECG) of standard limb lead Ⅱ was monitored and the heart was taken 4 h after reperfusion for examining myocardial infarcted size with triphenyl tetrazolium chloride (TTC) staining. In the second part, 48 SD rats were randomized into sham-operation, model, moxibustion and autophagy inhibitor (3-MA) groups, with 12 rats in each group. The serum cTnT level was assayed and histopathological changes of the myocardial tissue below the ligation site were examined with HE staining, and the expression levels of Bcl-2, Bax and Beclin 1 proteins in the myocardial tissue below the LAD-ligated site were detected using Western blot.

RESULTS

Compared with the model group, the percentages of myocardial infarcted area were significantly decreased in the 4 d, 5 d and 7 d Moxi groups (P<0.05), but without significant differences among the 3 Moxi groups (P>0.05). The state of MI-induced breakage and disordered arrangement of myocardial fibers with interstitial edema and inflammatory cell infiltration at the MI stage in the Moxi group and at the reperfusion stage in the autophagy inhibitor group was relatively lighter. The levels of serum cTnT content and Bax/Bcl-2 and Beclin 1 protein expression at the MI and reperfusion stages were significantly higher in the model group than in the sham-operation group (P<0.01), and considerably lower in the Moxi and autophagy groups than in the model group (P<0.01). The serum cTnT content, ratio of Bax/Bcl-2 expression and Beclin 1 expression levels at the MI and reperfusion stages were significantly lower in the autophagy inhibitor group than in the Moxi group (P<0.05).

CONCLUSIONS

Moxibustion with seed-sized moxa cones at "Neiguan" (PC 6) can effectively alleviate myocardial ischemia in MI/RI rats, which is probably related to its effect in down-regulating Bax/Bcl-2 and Beclin 1 expression and in inhibiting autophagy.

Authors+Show Affiliations

The Second Clinical Medical College, Nanjing University of Traditional Chinese Medicine, Nanjing 210023, China.The Second Clinical Medical College, Nanjing University of Traditional Chinese Medicine, Nanjing 210023, China.The Second Clinical Medical College, Nanjing University of Traditional Chinese Medicine, Nanjing 210023, China.The Second Clinical Medical College, Nanjing University of Traditional Chinese Medicine, Nanjing 210023, China.The Second Clinical Medical College, Nanjing University of Traditional Chinese Medicine, Nanjing 210023, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

29318850

Citation

Bai, Hua, et al. "[Effect of Moxibustion Preconditioning With Seed-sized Moxa Cones On Myocardial Infarction Size and Beclin 1 Expression in Myocardial Ischemia-reperfusion Injury Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 42, no. 6, 2017, pp. 471-6.
Bai H, Lu SF, Chen WY, et al. [Effect of Moxibustion Preconditioning with Seed-sized Moxa Cones on Myocardial Infarction Size and Beclin 1 Expression in Myocardial Ischemia-reperfusion Injury Rats]. Zhen Ci Yan Jiu. 2017;42(6):471-6.
Bai, H., Lu, S. F., Chen, W. Y., Zhong, Z. H., & Gu, Y. H. (2017). [Effect of Moxibustion Preconditioning with Seed-sized Moxa Cones on Myocardial Infarction Size and Beclin 1 Expression in Myocardial Ischemia-reperfusion Injury Rats]. Zhen Ci Yan Jiu = Acupuncture Research, 42(6), 471-6. https://doi.org/10.13702/j.1000-0607.2017.06.001
Bai H, et al. [Effect of Moxibustion Preconditioning With Seed-sized Moxa Cones On Myocardial Infarction Size and Beclin 1 Expression in Myocardial Ischemia-reperfusion Injury Rats]. Zhen Ci Yan Jiu. 2017 Dec 25;42(6):471-6. PubMed PMID: 29318850.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of Moxibustion Preconditioning with Seed-sized Moxa Cones on Myocardial Infarction Size and Beclin 1 Expression in Myocardial Ischemia-reperfusion Injury Rats]. AU - Bai,Hua, AU - Lu,Sheng-Feng, AU - Chen,Wan-Ying, AU - Zhong,Ze-Hao, AU - Gu,Yi-Huang, PY - 2018/1/11/entrez PY - 2018/1/11/pubmed PY - 2019/4/4/medline KW - Autophagy KW - Autophagy associated protein Beclin 1 KW - Moxibustion with seed-size moxa cone KW - Myocardial ischemia reperfusion SP - 471 EP - 6 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 42 IS - 6 N2 - OBJECTIVE: To observe the effect of moxibustion (Moxi) preconditioning with seed-sized moxa cones on myocardial ischemia-reperfusion injury (MI/RI) at different stages, and to analyze the correlation between this effect and the expression of autophagy related protein Beclin 1. METHODS: This study contains two parts: 1) changes of myocardial pathological injury and percentages of myocardial infarcted area at different time-points after modeling and Moxi intervention, and 2) effect of Moxi on contents of serum cardiac troponin T(cTnT) and expression of myocardial Bcl-2, Bax and Beclin 1 proteins. In the first part, 42 SD rats were randomly divided into model group, 1 day (d) Moxi group, 2 d Moxi group, 3 d Moxi group,4 d Moxi group, 5 d Moxi group and 7 d Moxi group. The model of MI/RI was established by ligating the left anterior descending coronary artery (LAD) for 30 min and reperfusion for 240 min. The electrocardiogram (ECG) of standard limb lead Ⅱ was monitored and the heart was taken 4 h after reperfusion for examining myocardial infarcted size with triphenyl tetrazolium chloride (TTC) staining. In the second part, 48 SD rats were randomized into sham-operation, model, moxibustion and autophagy inhibitor (3-MA) groups, with 12 rats in each group. The serum cTnT level was assayed and histopathological changes of the myocardial tissue below the ligation site were examined with HE staining, and the expression levels of Bcl-2, Bax and Beclin 1 proteins in the myocardial tissue below the LAD-ligated site were detected using Western blot. RESULTS: Compared with the model group, the percentages of myocardial infarcted area were significantly decreased in the 4 d, 5 d and 7 d Moxi groups (P<0.05), but without significant differences among the 3 Moxi groups (P>0.05). The state of MI-induced breakage and disordered arrangement of myocardial fibers with interstitial edema and inflammatory cell infiltration at the MI stage in the Moxi group and at the reperfusion stage in the autophagy inhibitor group was relatively lighter. The levels of serum cTnT content and Bax/Bcl-2 and Beclin 1 protein expression at the MI and reperfusion stages were significantly higher in the model group than in the sham-operation group (P<0.01), and considerably lower in the Moxi and autophagy groups than in the model group (P<0.01). The serum cTnT content, ratio of Bax/Bcl-2 expression and Beclin 1 expression levels at the MI and reperfusion stages were significantly lower in the autophagy inhibitor group than in the Moxi group (P<0.05). CONCLUSIONS: Moxibustion with seed-sized moxa cones at "Neiguan" (PC 6) can effectively alleviate myocardial ischemia in MI/RI rats, which is probably related to its effect in down-regulating Bax/Bcl-2 and Beclin 1 expression and in inhibiting autophagy. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/29318850/[Effect_of_Moxibustion_Preconditioning_with_Seed_sized_Moxa_Cones_on_Myocardial_Infarction_Size_and_Beclin_1_Expression_in_Myocardial_Ischemia_reperfusion_Injury_Rats]_ DB - PRIME DP - Unbound Medicine ER -