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Rapid detection of a dengue virus RNA sequence with single molecule sensitivity using tandem toehold-mediated displacement reactions.

Abstract

A DNA tetrahedron having a single-strand DNA edge was used to detect a dengue virus RNA sequence. Novel tandem toehold-mediated displacement reactions (tTMDR) were developed to amplify the fluorescence signal from the DNA tetrahedron. Using an excess of the DNA tetrahedron each target RNA was recycled about 103 times during the tTMDR process. This amplification process was used for the sensitive detection of dengue virus RNA in this study. As few as 6 copies of RNA per sample could be detected using a photon count technique with single molecule sensitivity.

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  • Authors+Show Affiliations

    ,

    Biodesign Center for BioEnergetics, Arizona State University, Tempe 85287, USA. Shengxi.Chen.1@asu.edu Sid.Hecht@asu.edu.

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    MeSH

    Base Sequence
    Biosensing Techniques
    DNA, Single-Stranded
    Dengue Virus
    Fluorescence
    Molecular Imaging
    Optical Imaging
    RNA, Viral
    Time Factors

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    29319084