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Immunoprofile of α-Gal- and B-antigen-specific responses differentiates red meat-allergic patients from healthy individuals.
Allergy. 2018 Jul; 73(7):1525-1531.A

Abstract

BACKGROUND

The galactose-α-1,3-galactose (α-Gal) epitope is involved in red meat allergy. As α-Gal is structurally similar to the blood group B-antigen, we explored the relationship between the immune responses to α-Gal- and the B-antigen in red meat-allergic patients compared to healthy A/O or B blood donors.

METHODS

Sera from 51 red meat-allergic patients IgE-positive to α-Gal and 102 healthy blood donors (51 blood group A/O; 51 blood group B) were included. α-Gal- and B-antigen-specific IgE (ImmunoCAP) and IgG/IgG1-4 (ELISA) responses were determined. Basophil activation tests were performed.

RESULTS

Fifteen healthy donors were IgE positive to α-Gal, of which 3 had blood group B. The allergic patients had significantly higher α-Gal IgE levels compared to the healthy donors. The majority of the allergic patients, but none of the healthy donors, had IgE against the B-antigen. Inhibition studies revealed cross-reactivity between α-Gal and the B-antigen. The biological activity of the B-antigen was confirmed by basophil activation tests. Anti-α-Gal IgG1 and IgG4 levels were significantly higher in the patients compared to the healthy donors. Moreover, the IgG response to the B-antigen was comparable between the allergic patients and healthy A/O donors.

CONCLUSION

Red meat-allergic patients showed significantly higher α-Gal IgE, IgG1 , and IgG4 levels, reflecting a Th2 response, compared to healthy blood donors. Blood group B donors had significantly reduced antibody responses to α-Gal, due to similarities with the B-antigen, resulting in a lower risk of sensitization to α-Gal and development of red meat allergy.

Authors+Show Affiliations

Department of Medicine Solna, Immunology and Allergy Unit, Karolinska Institutet and University Hospital, Stockholm, Sweden.Department of Clinical Immunology and Transfusion Medicine, Karolinska University Hospital, Stockholm, Sweden.Department of Medicine Solna, Immunology and Allergy Unit, Karolinska Institutet and University Hospital, Stockholm, Sweden.Department of Internal Medicine, Södersjukhuset, Stockholm, Sweden.Department of Medicine Solna, Immunology and Allergy Unit, Karolinska Institutet and University Hospital, Stockholm, Sweden.Department of Medicine Solna, Immunology and Allergy Unit, Karolinska Institutet and University Hospital, Stockholm, Sweden. Department of Clinical Immunology and Transfusion Medicine, Karolinska University Hospital, Stockholm, Sweden.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29319188

Citation

Apostolovic, D, et al. "Immunoprofile of α-Gal- and B-antigen-specific Responses Differentiates Red Meat-allergic Patients From Healthy Individuals." Allergy, vol. 73, no. 7, 2018, pp. 1525-1531.
Apostolovic D, Rodrigues R, Thomas P, et al. Immunoprofile of α-Gal- and B-antigen-specific responses differentiates red meat-allergic patients from healthy individuals. Allergy. 2018;73(7):1525-1531.
Apostolovic, D., Rodrigues, R., Thomas, P., Starkhammar, M., Hamsten, C., & van Hage, M. (2018). Immunoprofile of α-Gal- and B-antigen-specific responses differentiates red meat-allergic patients from healthy individuals. Allergy, 73(7), 1525-1531. https://doi.org/10.1111/all.13400
Apostolovic D, et al. Immunoprofile of α-Gal- and B-antigen-specific Responses Differentiates Red Meat-allergic Patients From Healthy Individuals. Allergy. 2018;73(7):1525-1531. PubMed PMID: 29319188.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunoprofile of α-Gal- and B-antigen-specific responses differentiates red meat-allergic patients from healthy individuals. AU - Apostolovic,D, AU - Rodrigues,R, AU - Thomas,P, AU - Starkhammar,M, AU - Hamsten,C, AU - van Hage,M, Y1 - 2018/02/05/ PY - 2017/12/22/accepted PY - 2018/1/11/pubmed PY - 2019/8/8/medline PY - 2018/1/11/entrez KW - B-antigen KW - IgE KW - allergenicity KW - red meat allergy KW - α-Gal SP - 1525 EP - 1531 JF - Allergy JO - Allergy VL - 73 IS - 7 N2 - BACKGROUND: The galactose-α-1,3-galactose (α-Gal) epitope is involved in red meat allergy. As α-Gal is structurally similar to the blood group B-antigen, we explored the relationship between the immune responses to α-Gal- and the B-antigen in red meat-allergic patients compared to healthy A/O or B blood donors. METHODS: Sera from 51 red meat-allergic patients IgE-positive to α-Gal and 102 healthy blood donors (51 blood group A/O; 51 blood group B) were included. α-Gal- and B-antigen-specific IgE (ImmunoCAP) and IgG/IgG1-4 (ELISA) responses were determined. Basophil activation tests were performed. RESULTS: Fifteen healthy donors were IgE positive to α-Gal, of which 3 had blood group B. The allergic patients had significantly higher α-Gal IgE levels compared to the healthy donors. The majority of the allergic patients, but none of the healthy donors, had IgE against the B-antigen. Inhibition studies revealed cross-reactivity between α-Gal and the B-antigen. The biological activity of the B-antigen was confirmed by basophil activation tests. Anti-α-Gal IgG1 and IgG4 levels were significantly higher in the patients compared to the healthy donors. Moreover, the IgG response to the B-antigen was comparable between the allergic patients and healthy A/O donors. CONCLUSION: Red meat-allergic patients showed significantly higher α-Gal IgE, IgG1 , and IgG4 levels, reflecting a Th2 response, compared to healthy blood donors. Blood group B donors had significantly reduced antibody responses to α-Gal, due to similarities with the B-antigen, resulting in a lower risk of sensitization to α-Gal and development of red meat allergy. SN - 1398-9995 UR - https://www.unboundmedicine.com/medline/citation/29319188/Immunoprofile_of_α_Gal__and_B_antigen_specific_responses_differentiates_red_meat_allergic_patients_from_healthy_individuals_ L2 - https://doi.org/10.1111/all.13400 DB - PRIME DP - Unbound Medicine ER -