Prime

Type your tag names separated by a space and hit enter

Field effectiveness of highly pathogenic avian influenza H5N1 vaccination in commercial layers in Indonesia.

Abstract

Although vaccination of poultry for control of highly pathogenic avian influenza virus (HPAIV) H5N1 has been practiced during the last decade in several countries, its effectiveness under field conditions remains largely unquantified. Effective HPAI vaccination is however essential in preventing incursions, silent infections and generation of new H5N1 antigenic variants. The objective of this study was to asses the level and duration of vaccine induced immunity in commercial layers in Indonesia. Titres of H5N1 haemagglutination inhibition (HI) antibodies were followed in individual birds from sixteen flocks, age 18-68 week old (wo). The study revealed that H5N1 vaccination had highly variable outcome, including vaccination failures, and was largely ineffective in providing long lasting protective immunity. Flocks were vaccinated with seven different vaccines, administer at various times that could be grouped into three regimes: In regime A, flocks (n = 8) were vaccinated two or three times before 19 wo; in regime B (n = 2), two times before and once after 19 wo; and in regime C (n = 6) three to four times before and two to three times after 19 wo. HI titres in regime C birds were significantly higher during the entire observation period in comparison to titres of regime A or B birds, which also differed significantly from each other. The HI titres of individual birds in each flock differed significantly from birds in other flocks, indicating that the effectiveness of field vaccination was highly variable and farm related. Protective HI titres of >4log2, were present in the majority of flocks at 18 wo, declined thereafter at variable rate and only two regime C flocks had protective HI titres at 68 wo. Laboratory challenge with HPAIV H5N1 of birds from regime A and C flocks confirmed that protective immunity differed significantly between flocks vaccinated by these two regimes. The study revealed that effectiveness of the currently applied H5N1 vaccination could be improved and measures to achieve this are discussed.

Links

  • PMC Free PDF
  • PMC Free Full Text
  • FREE Publisher Full Text
  • Authors+Show Affiliations

    ,

    Indonesian Research Centre for Veterinary Science, Bogor, Indonesia.

    ,

    Faculty of Veterinary Science, Universitas Gadjah Mada, Yogyakarta, Indonesia.

    ,

    Indonesian Research Centre for Veterinary Science, Bogor, Indonesia.

    ,

    Indonesian Research Centre for Veterinary Science, Bogor, Indonesia.

    ,

    Faculty of Veterinary Science, Universitas Gadjah Mada, Yogyakarta, Indonesia.

    ,

    Directorate General of Livestock and Animal Health Services, Jakarta, Indonesia.

    ,

    CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia.

    ,

    Faculty of Veterinary Science, Universitas Gadjah Mada, Yogyakarta, Indonesia.

    ,

    School of Information Technology and Mathematical Sciences, Division of Information Technology, Engineering and the Environment, University of South Australia, Adelaide, South Australia, Australia.

    ,

    School of Veterinary and Agricultural Sciences, University of Melbourne, Melbourne, Victoria, Australia.

    School of Veterinary and Agricultural Sciences, University of Melbourne, Melbourne, Victoria, Australia.

    Source

    PloS one 13:1 2018 pg e0190947

    MeSH

    Animal Husbandry
    Animals
    Antibodies, Viral
    Chickens
    Hemagglutination Inhibition Tests
    Indonesia
    Influenza A Virus, H5N1 Subtype
    Influenza Vaccines
    Influenza in Birds
    Longitudinal Studies
    Poultry Diseases
    Prospective Studies
    Treatment Failure

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    29320563