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Cranberry proanthocyanidin-chitosan hybrid nanoparticles as a potential inhibitor of extra-intestinal pathogenic Escherichia coli invasion of gut epithelial cells.
Int J Biol Macromol 2018; 111:415-420IJ

Abstract

Chitosan interacts with proanthocyanidins through hydrogen-bonding, which allows encapsulation and development of stable nanoparticles via ionotropic gelation. Cranberry proanthocyanidins (PAC) are associated with the prevention of urinary tract infections and PAC inhibit invasion of gut epithelial cells by extra-intestinal pathogenic Escherichia coli (ExPEC). We determined the effect of cranberry proanthocyanidin-chitosan hybrid nanoparticles (PAC-CHTNp) on the ExPEC invasion of gut epithelial cells in vitro. PAC-CHTNp were characterized according to size, morphology, and bioactivity. Results showed a decrease in the size of the nanoparticles as the concentration of PAC was increased, indicating that PAC increases cross-linking by hydrogen-bonding on the surface of the chitosan nanoparticles. Nanoparticles were produced with diameters ranging from 367.3 nm to 293.2 nm. Additionally, PAC-CHTNp significantly inhibited the ability of ExPEC to invade the enterocytes by ~80% at 66 μg GAE/mL and by ~92% at 100 μg GAE/mL. Results also indicate that chitosan nanoparticles alone were not significantly different from controls in preventing ExPEC invasion of enterocytes (data not shown) and also there were not significant differences between PAC alone and PAC-CHTNp, suggesting that the new PAC-CHTNp could lead to an increase in the stability of encapsulated PAC, maintain the molecular adhesion of PAC to ExPEC.

Authors+Show Affiliations

Material Sciences & Engineering, University of Wisconsin-Madison, 1415 Engineering Dr, Madison, WI 53706, United States; Reed Research Group, Department of Animal Sciences, University of Wisconsin-Madison, 1675 Observatory Dr, Madison, WI 53706, United States.Reed Research Group, Department of Animal Sciences, University of Wisconsin-Madison, 1675 Observatory Dr, Madison, WI 53706, United States.Reed Research Group, Department of Animal Sciences, University of Wisconsin-Madison, 1675 Observatory Dr, Madison, WI 53706, United States. Electronic address: sergio.carballo@wisc.edu.Reed Research Group, Department of Animal Sciences, University of Wisconsin-Madison, 1675 Observatory Dr, Madison, WI 53706, United States.Material Sciences & Engineering, University of Wisconsin-Madison, 1415 Engineering Dr, Madison, WI 53706, United States; Reed Research Group, Department of Animal Sciences, University of Wisconsin-Madison, 1675 Observatory Dr, Madison, WI 53706, United States.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29325748

Citation

Alfaro-Viquez, Emilia, et al. "Cranberry Proanthocyanidin-chitosan Hybrid Nanoparticles as a Potential Inhibitor of Extra-intestinal Pathogenic Escherichia Coli Invasion of Gut Epithelial Cells." International Journal of Biological Macromolecules, vol. 111, 2018, pp. 415-420.
Alfaro-Viquez E, Esquivel-Alvarado D, Madrigal-Carballo S, et al. Cranberry proanthocyanidin-chitosan hybrid nanoparticles as a potential inhibitor of extra-intestinal pathogenic Escherichia coli invasion of gut epithelial cells. Int J Biol Macromol. 2018;111:415-420.
Alfaro-Viquez, E., Esquivel-Alvarado, D., Madrigal-Carballo, S., Krueger, C. G., & Reed, J. D. (2018). Cranberry proanthocyanidin-chitosan hybrid nanoparticles as a potential inhibitor of extra-intestinal pathogenic Escherichia coli invasion of gut epithelial cells. International Journal of Biological Macromolecules, 111, pp. 415-420. doi:10.1016/j.ijbiomac.2018.01.033.
Alfaro-Viquez E, et al. Cranberry Proanthocyanidin-chitosan Hybrid Nanoparticles as a Potential Inhibitor of Extra-intestinal Pathogenic Escherichia Coli Invasion of Gut Epithelial Cells. Int J Biol Macromol. 2018;111:415-420. PubMed PMID: 29325748.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cranberry proanthocyanidin-chitosan hybrid nanoparticles as a potential inhibitor of extra-intestinal pathogenic Escherichia coli invasion of gut epithelial cells. AU - Alfaro-Viquez,Emilia, AU - Esquivel-Alvarado,Daniel, AU - Madrigal-Carballo,Sergio, AU - Krueger,Christian G, AU - Reed,Jess D, Y1 - 2018/01/08/ PY - 2017/11/28/received PY - 2018/01/03/revised PY - 2018/01/05/accepted PY - 2018/1/13/pubmed PY - 2018/8/30/medline PY - 2018/1/13/entrez KW - Chitosan KW - Extra-intestinal pathogenic Escherichia coli KW - Proanthocyanidins SP - 415 EP - 420 JF - International journal of biological macromolecules JO - Int. J. Biol. Macromol. VL - 111 N2 - Chitosan interacts with proanthocyanidins through hydrogen-bonding, which allows encapsulation and development of stable nanoparticles via ionotropic gelation. Cranberry proanthocyanidins (PAC) are associated with the prevention of urinary tract infections and PAC inhibit invasion of gut epithelial cells by extra-intestinal pathogenic Escherichia coli (ExPEC). We determined the effect of cranberry proanthocyanidin-chitosan hybrid nanoparticles (PAC-CHTNp) on the ExPEC invasion of gut epithelial cells in vitro. PAC-CHTNp were characterized according to size, morphology, and bioactivity. Results showed a decrease in the size of the nanoparticles as the concentration of PAC was increased, indicating that PAC increases cross-linking by hydrogen-bonding on the surface of the chitosan nanoparticles. Nanoparticles were produced with diameters ranging from 367.3 nm to 293.2 nm. Additionally, PAC-CHTNp significantly inhibited the ability of ExPEC to invade the enterocytes by ~80% at 66 μg GAE/mL and by ~92% at 100 μg GAE/mL. Results also indicate that chitosan nanoparticles alone were not significantly different from controls in preventing ExPEC invasion of enterocytes (data not shown) and also there were not significant differences between PAC alone and PAC-CHTNp, suggesting that the new PAC-CHTNp could lead to an increase in the stability of encapsulated PAC, maintain the molecular adhesion of PAC to ExPEC. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/29325748/Cranberry_proanthocyanidin_chitosan_hybrid_nanoparticles_as_a_potential_inhibitor_of_extra_intestinal_pathogenic_Escherichia_coli_invasion_of_gut_epithelial_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(17)34737-2 DB - PRIME DP - Unbound Medicine ER -