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Antibacterial effects of cinnamon (Cinnamomum zeylanicum) bark essential oil on Porphyromonas gingivalis.
Microb Pathog. 2018 Mar; 116:26-32.MP

Abstract

The objective of this study was to investigate the antibacterial effects of cinnamon (Cinnamomum zeylanicum) bark essential oil (CBEO) and its principal constituent cinnamaldehyde against Porphyromonas gingivalis and to elucidate the antibacterial mechanism. GC-MS analysis showed that cinnamaldehyde was the major constituent in CBEO (57.97%). The minimum inhibition concentrations (MICs) of CBEO and cinnamaldehyde were 6.25 μg/mL and 2.5 μM for P. gingivalis, respectively. Nucleic acid and protein leakage was observed with increasing concentrations of CBEO and cinnamaldehyde. Additionally, propidium iodide uptake assays revealed CBEO and cinnamaldehyde at 1 × MIC impaired P. gingivalis membrane integrity by enhancing cell permeability. Morphological changes in P. gingivalis cells were observed by scanning electron microscopy, which indicated cell membrane destruction. To further determine the anti-biofilm effect, relative biofilm formation and established biofilms were examined, which demonstrated that both CBEO and cinnamaldehyde at sub-MIC levels inhibited P. gingivalis biofilm formation by 74.5% and 67.3% separately, but only CBEO slightly decreased established biofilms by 33.5% at 4 × MIC. These results suggest the potential of CBEO as a natural antimicrobial agent against periodontal disease. Furthermore, cinnamaldehyde was confirmed to be the antibacterial substance of CBEO with inhibitory action against P. gingivalis.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Wang Y
Shanghai Institute of Technology, Shanghai 201418, China.
Zhang Y
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Shi YQ
Shanghai Institute of Technology, Shanghai 201418, China.
Pan XH
Shanghai Institute of Technology, Shanghai 201418, China.
Lu YH
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Cao P
Shanghai Jahwa (Group) Co., Ltd., Shanghai 200082, China. Electronic address: caoping@jahwa.com.cn.

MeSH

AcroleinAnti-Bacterial AgentsCell MembraneCinnamomum zeylanicumGas Chromatography-Mass SpectrometryHumansMicrobial Sensitivity TestsMicroscopy, Electron, ScanningOils, VolatilePermeabilityPlant BarkPorphyromonas gingivalis

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29325862

Citation

Wang, Yue, et al. "Antibacterial Effects of Cinnamon (Cinnamomum Zeylanicum) Bark Essential Oil On Porphyromonas Gingivalis." Microbial Pathogenesis, vol. 116, 2018, pp. 26-32.
Wang Y, Zhang Y, Shi YQ, et al. Antibacterial effects of cinnamon (Cinnamomum zeylanicum) bark essential oil on Porphyromonas gingivalis. Microb Pathog. 2018;116:26-32.
Wang, Y., Zhang, Y., Shi, Y. Q., Pan, X. H., Lu, Y. H., & Cao, P. (2018). Antibacterial effects of cinnamon (Cinnamomum zeylanicum) bark essential oil on Porphyromonas gingivalis. Microbial Pathogenesis, 116, 26-32. https://doi.org/10.1016/j.micpath.2018.01.009
Wang Y, et al. Antibacterial Effects of Cinnamon (Cinnamomum Zeylanicum) Bark Essential Oil On Porphyromonas Gingivalis. Microb Pathog. 2018;116:26-32. PubMed PMID: 29325862.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibacterial effects of cinnamon (Cinnamomum zeylanicum) bark essential oil on Porphyromonas gingivalis. AU - Wang,Yue, AU - Zhang,Yi, AU - Shi,Yan-Qin, AU - Pan,Xian-Hua, AU - Lu,Yan-Hua, AU - Cao,Ping, Y1 - 2018/01/09/ PY - 2017/10/26/received PY - 2018/01/05/revised PY - 2018/01/07/accepted PY - 2018/1/13/pubmed PY - 2018/8/31/medline PY - 2018/1/13/entrez KW - Antibacterial mechanism KW - Cinnamaldehyde KW - Cinnamon bark essential oil KW - Periodontal disease KW - Porphyromonas gingivalis SP - 26 EP - 32 JF - Microbial pathogenesis JO - Microb Pathog VL - 116 N2 - The objective of this study was to investigate the antibacterial effects of cinnamon (Cinnamomum zeylanicum) bark essential oil (CBEO) and its principal constituent cinnamaldehyde against Porphyromonas gingivalis and to elucidate the antibacterial mechanism. GC-MS analysis showed that cinnamaldehyde was the major constituent in CBEO (57.97%). The minimum inhibition concentrations (MICs) of CBEO and cinnamaldehyde were 6.25 μg/mL and 2.5 μM for P. gingivalis, respectively. Nucleic acid and protein leakage was observed with increasing concentrations of CBEO and cinnamaldehyde. Additionally, propidium iodide uptake assays revealed CBEO and cinnamaldehyde at 1 × MIC impaired P. gingivalis membrane integrity by enhancing cell permeability. Morphological changes in P. gingivalis cells were observed by scanning electron microscopy, which indicated cell membrane destruction. To further determine the anti-biofilm effect, relative biofilm formation and established biofilms were examined, which demonstrated that both CBEO and cinnamaldehyde at sub-MIC levels inhibited P. gingivalis biofilm formation by 74.5% and 67.3% separately, but only CBEO slightly decreased established biofilms by 33.5% at 4 × MIC. These results suggest the potential of CBEO as a natural antimicrobial agent against periodontal disease. Furthermore, cinnamaldehyde was confirmed to be the antibacterial substance of CBEO with inhibitory action against P. gingivalis. SN - 1096-1208 UR - https://www.unboundmedicine.com/medline/citation/29325862/Antibacterial_effects_of_cinnamon__Cinnamomum_zeylanicum__bark_essential_oil_on_Porphyromonas_gingivalis_ DB - PRIME DP - Unbound Medicine ER -