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Establishment of TUSMi003-A, an induced pluripotent stem cell (iPSC) line from a 62-year old Chinese Han patient with Alzheimer's disease with ApoE3/4 genetic background.

Abstract

A 62-year old Chinese Han Alzheimer's disease (AD) female patient with ApoE3/4 genetic background donated her Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system was used to reprogrammed PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in AD.

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  • Authors+Show Affiliations

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    Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

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    Department of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

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    Department of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

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    Department of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

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    Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

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    Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

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    IxCell Biotechnology Co., Ltd, Shanghai, China.

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    Department of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China. Electronic address: ligang@tongji.edu.cn.

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    Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China. Electronic address: ying.lei@tongji.edu.cn.

    Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

    Source

    Stem cell research 27: 2018 Mar pg 57-60

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    29328972